Identification and antifungal susceptibility of candida isolates from various clinical specimens in doctor Behçet uz hospital

The purpose of this study was to identify and determine antifungal susceptibility patterns of 101 yeast strains isolated from varios clinical specimens. Identification of the isolates were determined by API 20 C AUX kit (Biomérieux, France) and antifungal susceptibilities of these species to flucytosine, amphotericin B, fluconazole, itraconazole and voriconazole were determined by API ATB Fungus kit (Biomérieux, France). Of the 101 strains, 48 were isolated from urine, 46 from blood, 5 from catheter and 2 from wound. C.albicans (54.46%) was the most frequently isolated species, followed by C.parapsilosis (33.66%), C.famata (6.93%), C.tropicalis (2.97%) and C.pelliculosa (1.98%). High rates of resistance against itraconazole, fluconazole and voricanozole were detected in C.albicans. Different rates of resistance against especially itraconazole were detected in other Candida strains. Species definition and determination of antifungal susceptibility patterns are advised for the proper treatment of Candida infections

Antifungal Effects of Lactobacillus spp. Bacteria on Candida Yeast

WOS: 000285216200027Thirty Lactobacillus were isolated from the stool of 5-15 year-old children using MRS agar and identified by API 50CHL. Also. 50 Candida were isolated from blood cultures using SD agar and identified by Vitek 2 instrument with YST kit. Antifungal effects of Lactobacillus bacteria on Candida were explored using well diffusion method in SD agar. Lactobacillus had the most prominent antifungal effect against on C. albicans (M29, M36), C. parapsliosis (M25, M26), C. famata (M28) and C. guilliermondii (M38) yeasts. This study shows the presence of antifungal effect of Lactobacillus on Candida isolated from blood cultures

Antifungal effects of Lactobacillus spp. bacteria on Candida yeast [Lactobacillus bakterilerinin Candida'lar üzerine antifungal etkileri]

Thirty Lactobacillus were isolated from the stool of 5-15 year-old children using MRS agar and identified by API 50CHL. Also, 50 Candida were isolated from blood cultures using SD agar and identified by Vitek 2 instrument with YST kit. Antifungal effects of Lactobacillus bacteria on Candida were explored using well diffusion method in SD agar. Lactobacillus had the most prominent antifungal effect against on C. albicans (M29, M36), C. parapsilosis (M25, M26), C. famata (M28) and C. guilliermondii (M38) yeasts. This study shows the presence of antifungal effect of Lactobacillus on Candida isolated from blood cultures

Effects of different temperatures on virulence factors and antimicrobial resistance of Aeromonas isolated from retail food

WOS: 000276472700003The aim of this study was to evaluate the public health significance of representative motile Aeromonas isolates, which were mainly isolated from different foods, and to determine the effects of different temperatures on virulence factors such as hemolysin, protease, lipase and nuclease. The influence of different temperatures (+4 degrees C, +25 degrees C and +37 degrees C) on virulence factors of 40 isolates of Aeromonas (A.) spp. (A. hydrophila, A. caviae and A. sobria) was investigated. All isolates could produce hemolysin, protease, lipase, and nuclease at +37 degrees C, +25 degrees C and +4 degrees C; however, these virulence associated factors were produced faster at +37 degrees C and +25 C than at +4 C. All Aeromonas species were also tested for antibiotic resistance patterns and were found to be resistant to ampicillin, yet sensitive to imipenem, ciprofloxacin and amikacin antibiotics.Gazi University Research FundGazi University [0.24/2002-0-1]This work was financially supported by the Gazi University Research Fund (Project No, Proje N0.24/2002-0-1)

sourdough processes

The main purpose of this study was to obtain antimicrobial starter cultures for sourdough processes. For this purpose, 60 sourdough samples were collected from different bakery plants in Usak. The chemical and microbiological properties of these samples were analyzed. Lactic acid bacteria strains having antimicrobial activity were isolated and identified. The metabolic properties (total amount of acids, organic acids and diacetyl produced and the properties of proteolytic and amylolytic enzymes activity) of the selected strains were determined. The strains with best potential as sourdough starters were L. brevis ssp. lindneri 2103, L. viridenscens 241, 242, Pediococcus sp. E5 and L. delbrueckii F5. (c) 2005 Elsevier Ltd. All rights reserved

properties of Lactobacillus spp. isolated from human milk

This study aims to determine the effects of inulin and fructo-oligosaccharide (FOS) on the probiotic properties of five Lactobacillus spp. isolated from human milk. Lactobacillus spp. were isolated and identified, and the growth characteristics, acid and bile salt tolerance, antagonistic effects, and cholesterol assimilation of Lactobacillus strains were investigated in the presence of inulin and FOS. Lactobacillus casei L1 was able to utilize inulin and FOS as carbon source as well as glucose even other strains were able to use, including Lactobacillus rhamnosus GG. This strain also showed high tolerance to acid and bile salt, even at pH 2.5 and 0.5% bile salt levels, respectively. Inulin and FOS promoted the antimicrobial activity of L. casei L1 against pathogenic bacteria. Cholesterol assimilation was higher than in the other and control probiotic strains in the presence inulin and FOS, which were measured as 14 and 25 mg/dL, respectively. In conclusion, L. casei L1 can use both inulin and FOS to maintain its viability both at digestive conditions and also the relevant prebiotics, and show broad antagonistic activity and cholesterol assimilation

from tulum cheese

The aim of this study was to characterize the probiotic characteristics of Lactobacillus fermentum strains isolated from Tulum cheese. Seven L fermentum strains were selected among the isolated and identified lactobacillus strains due to their abundance. When the gastric condition was considered, L. fermentum LP3 and LP4 were able to tolerate pH 2.5 and 1% bile salt. All L. fermentum strains had similar enzymatic activity and antibiotic resistance pattern but the highest antagonistic effect was detected within LP3, LP4 and LP6. Cholesterol assimilation amount of L. fermentum strains ranged between 12.1 and 45.3% in MRS and 20.7-71.1% in MRS with bile. The highest cholesterol assimilation in MRS and MRS with bile was occurred by LP3 and LP4, respectively. L fermentum LP2 adhered to caco-2 cells more than Lactobacillus rhamnosus LGG where LP3, LP4 and LP5 adhered at similar level. In conclusion, L fermentum LP3 and LP4 fulfilled sufficient criteria to be probiotics for use as a starter culture in the production of tulum cheese or other dairy products. Also this study indicated that some food-associated Lactobacillus strains non-predominant for gut biota have significant probiotic potential. (C) 2014 Elsevier Ltd. All rights reserved

Probiotic properties of lactobacilli species isolated from children's feces

In the present research, the 20 lactobacilli isolated from children feces aged 4-15 years old were investigated for their capabilities to survive at pH 2.0, 2.5, 3.0 and in the presence of 0.25, 0.50 and 0.75% bile salts, their effect on the growth of pathogens, in addition to their sensitivity against 13 selected antibiotics. All the lactobacilli strains were able to survive in low pH and bile salt conditions at pH 2.0 and 0.25% bile salt for 2h. Moreover, all lactobacilli strains exhibited inhibitory activity against Escherichia coli ATCC 11229, Pseudomonas aeruginosa ATCC 27853 and Staphylococcus aureus ATCC 29213. In addition, all lactobacilli strains indicated resistance to teicoplanin, vancomycin, and bacitracin. The amount of exopolysaccharide (EPS) produced by the strains was 70 and 290mg/L. The capabilities to autoaggregation and coaggregate with E. coli ATCC 11229 of the strains were also evaluated. High EPS-producing strains indicated significant autoaggregation and coaggregation capability with test bacteria (p<0.01). The maximum cholesterol removal (76.5%) was observed by strain Lactobacillus pentosus T3, producing a high amount of exopolysaccharide, in 0.3%oxgall concentration (p<0.05). Our results demonstrate that the capability to EPS production, acid-bile tolerance, antimicrobial activity, antibiotic resistance, aggregation and cholesterol removal of lactobacilli could be utilized for preliminary screening in order to identify potentially probiotic bacteria suitable for human. © 2013 Elsevier Ltd

Effect of Heavy Metals and Antibiotics on Siderophores Producing Bacterial Isolates

2nd International Conference on Computational and Experimental Science and Engineering (ICCESEN) -- OCT 14-19, 2015 -- Kemer, TURKEYWOS: 000384810700047In this study, siderophore-producing bacterial species have been isolated from soils contaminated with diesel oil. In P. aeruginosa (P-7) production of siderophores was inhibited at concentrations of ZnSO4 and CoCl2 of 2000 mu M, while B. subtilis (BS-1) has increased siderophores production at similar concentrations of CoCl 2 and ZnSO4. The same bacteria have increased the production of siderophores in presence of streptomycine (512 mg/ml). In B. subtilis the production of siderophores was inhibited by cefuroxime. The results show the effect of metals on the efficiency of siderophore production by bacteria for potential application in bioremediation of metalcontaminated iron-deficient soils in the microbial assisted phytoremediation processes. The results include the effect of metals on siderophore production efficiency of the bacteria used for the bioremediation of metal-contaminated soils. In conclusion, it has been found that heavy metals and antibiotics significantly effect the production of siderophores by bacteria

Investigation of probiotic properties of lactobacilli bacteria isolated from human gastrointestinal tract

WOS: 000366008400005In this study, the probiotic characteristics of Lactobacillus which were taken from the stool samples of 30 children, aged between 5 and 15 years, were studied. The stomach medium (low pH) and bile salt tolerance, bile salt hydrolysis activity, antagonistic activity and cholesterol assimilation quantity of Lactobacillus strains were determined. It was defined that on the whole Lactobacillus strains were resistant to high acidity (pH 2.0-2.51 and sensitive to high levels of bile salt 1% 1.00 Oxgall), there was no bile salt hydrolysis activity, they were resistant to vancomycin, teicoplanin and bacitracin and had antagonistic effects on pathogenic bacteria. It was noted that only L. curvatus L26 strain, which produced bacteriocin, had a general antagonistic effect; was indulgent to gastric acid and bile salt; and in different bile salt medium assimilated cholesterol at a high degree 115.22-25.42 mu g/ml). At the same time, the fact that L. curvatus L26 had beta-glucuronidase digesting prebiotics and beta-galactosidase digesting lactose is one of its most significant probiotic properties. In the light of this information, our opinion is that L. curvatus L26 can involve potential probiotic characteristics