Amanita longitibiale- A new species of Amanita section Phalloideae from central México and Southeastern USA

Amanita longitibiale is described as new from central Mexico and North Carolina, U.S.A. In Amanita section Phalloideae, it has the unique combination of cylindric spores, pigmented pileipellis, slender and radicating stipe bulb, and saccate volva

Molecular phylogenetic analyses show that Amanita ovoidea and Amanita proxima are distinct species and suggest their assignment to Roanokenses section

Amanita ovoidea is generally considered edible and is morphologically similar to Amanita proxima that contains nephrotoxins. Both species were assigned to Amanita section Amidella. To study their phylogenetic relationships, we performed nrITS- and RPB2-based analyses of these and other Amanita species. Our analyses show that (i) A. ovoidea and A. proxima are distinct species; (ii) A. ovoidea has an extensively heterogeneous ITS with up to 4.4% pairwise genetic distance between individuals; (iii) in A. ovoidea, pairwise genetic distance between ITS clones from a single basidiome was as high as 3.2%, possibly because of hybridization events and subsequent failure of the rDNA repeats to homogenize; (iv) A. aminoaliphatica and A. proxima are taxonomic synonyms; and (v) A. proxima and A. ovoidea cluster with species belonging to section Roanokenses rather than with those of section Amidella sensu stricto. This study supports the development of species-specific markers for diagnosis of the toxic and the edible species here examined. © 2019, German Mycological Society and Springer-Verlag GmbH Germany, part of Springer Nature