A Histologic and Histomorphometric Analysis of Bone Tissue Regeneration with Perforated Bone Allograft in Rabbit Femur Defect

AIM: The aim of this study is to provide a comparative histopathological evaluation of the regeneration of bone defect filling with perforated antibiotic-impregnated bone allograft. MATERIALS AND METHODS: Seventy-two healthy rabbits (24 rabbits in each group) were used for this study. Bone defects (3-mm diameter, 10-mm depth) were created in the femur. Human femoral head prepared according to the Marburg bone bank system was used as a bone allograft. The control group did not receive any filling. The experimental groups were as follows: Group 1 – the defects were filled with bone allografts and Group 2 – Perforated gentamycin-impregnated bone allografts. The animals were euthanized after 14, 30, and 60 days. Evaluations consisted of histology at 14-, 30-, and 60-days post-surgery. RESULTS: A mature bone formation in the group without a bone allograft occurred after 30 days and the group with an allograft after 14 days. In the groups with an allograft, a bone marrow defect was noted as complete closure after 30 days. Histomorphometric analysis showed that in the group with an antibiotic-impregnated bone, allograft leads to increased resorption of the allograft in the intramedullary space compared to group without antibiotic. CONCLUSION: We believe that a perforated allograft as a result of clinical trials may be obvious and economically affordable in the treatment of bone defects. The use of gentamycin-impregnated bone allografts may be of value in the prevention and treatment of bone infections

Effect of autologous platelet-rich plasma on new bone formation and viability of a Marburg bone graft

This study aimed to compare the new bone formation, the process of remodeling, and the viability of bone grafts, using a combination of platelet-rich plasma (PRP) and Marburg bone graft versus bone grafts without any additional elements. For this study, 48 rabbits (with 24 rabbits in each group) were used. Bone defects were made in the femur, and the bone graft used was the human femoral head prepared according to the Marburg Bone Bank. Rabbits were divided into the following groups: heat-treated bone graft (HTBG group) and HTBG with PRP (HTBG + PRP group). After 14, 30, and 60 days post-surgery, the assessment of the results involved X-ray, histopathological, and histomorphometric analyses. The greater new bone formation was detected in the HTBG + PRP group on the 14 and 30 day (p < 0.001). Furthermore, the group using bone grafts with PRP demonstrated notably enhanced remodeling, characterized by stronger bone integration, more significant graft remineralization, and a circular pattern of newly formed bone. The PRP–bone graft complex improves bone tissue repair in the bone defect in the initial stages of bone regeneration. PRP has been identified to enhance the remodeling process and amplify the osteoconductive and osteoinductive capabilities of HTBGs

Radiostrontium contamination of soil and vegetation within the Semipalatinsk test site

The Semipalatinsk nuclear test site (STS) in the Republic of Kazakhstan was an important site for testing atomic bombs and other civil and military nuclear devices of the former Soviet Union. Results are presented from investigations on the extent of radiostrontium contamination in soils and vegetation at the technical areas of the STS, where the tests were conducted and in pastures used by farmers for grazing animals or for hay production. Our data are compared with those reported largely in the recent Russian language literature that has been reviewed. The extent of 90Sr contamination of soil is highly variable over the STS with the highest values associated with the technical areas, particularly the Degelen mountains. Recently measured values in both the present data and the Russian language literature confirm the relatively high current contamination of soil and vegetation in the vicinity of tunnels and associated watercourses in the Degelen area. The proportion of 90Sr in soil which could not be extracted with 6 M HCl was only an average of 20%, which is low compared to other test site areas and possibly indicates a relatively high mobility in this area, because the 90Sr is derived from leakage from explosion tunnels along watercourses rather than being associated with fused silicates. A comparison of relative activity concentrations in soil and vegetation suggests that the transfer of 90Sr to vegetation on the STS is high compared to that of 137Cs and plutonium

Эндопротез тазобедренного сустава модели КазНИИТО

The analysis of results of treatment of 24 patients with a pathology of hip joint by endoprosthesis of KazSRITO are presented. Intrasurgical monitoring of intraosteal pressure was measured. During implantation of a femoral component, the significant decrease of intramedullary pressure on the average is noted. At all patients of a wound have begun to live primarily, complications were not observed.Представлен анализ результатов эндопротезирования 24 больных с патологией тазобедренного сустава протезами КазНИИТО. Интраоперационно проводился мониторинг внутрикостного давления. Во время имплантации бедренного компонента отмечено его достоверное снижение. У всех пациентов раны зажили первично, осложнений не было