More Than Just Pulling Weeds: A Case Study of Engaging Upper- Division Conservation Biology Students in Service-Learning

The field of conservation biology focuses on maintaining biodiversity by mitigating both global and local threats. One of the top threats to biodiversity is the worldwide problem of invasive species. Each community has its own pests to control, and students can engage with this global issue on a local scale through well-designed service-learning courses. This article discusses how students engaged with conservation biology through an integrated service-learning project tackling invasive species at a local nature center. Products of the class, data from class artifacts, as well as student feedback on evaluations are also presented

More Than Just Pulling Weeds : A Case Study of Engaging Upper-Division Conservation Biology Students in Service-Learning

The field of conservation biology focuses on maintaining biodiversity by mitigating both global and local threats. One of the top threats to biodiversity is the worldwide problem of invasive species. Each community has its own pests to control, and students can engage with this global issue on a local scale through well-designed service-learning courses. This article discusses how students engaged with conservation biology through an integrated service-learning project tackling invasive species at a local nature center. Products of the class, data from class artifacts, as well as student feedback on evaluations are also presented

Characterization of highly informative cross-species microsatellite panels for the Australian dugong (Dugong dugon) and Florida manatee (Trichechus manatus latirostris) including five novel primers

The Australian dugong (Dugong dugon) and Florida manatee (Trichechus manatus latirostris) are threatened species of aquatic mammals in the order Sirenia. Sirenian conservation and management actions would benefit from a more complete understanding of genetic diversity and population structure. Generally, species-specific microsatellite markers are employed in conservation genetic studies; however, robust markers can be difficult and costly to isolate. To increase the number of available markers, dugong and manatee microsatellite primers were evaluated for cross-species amplification. Furthermore, one manatee and four dugong novel primers are reported. After polymerase chain reaction optimization, 23 (92%) manatee primers successfully amplified dugong DNA, of which 11 (48%) were polymorphic. Of the 32 dugong primers tested, 27 (84%) yielded product in the manatee, of which 17 (63%) were polymorphic. Dugong and manatee primers were compared and the most informative markers were selected to create robust and informative marker-panels for each species. These cross-species microsatellite marker-panels can be employed to assess other sirenian populations and can provide beneficial information for the protection and management of these unique mammals

Diversity of Environmental Single-Stranded DNA Phages Revealed by PCR Amplification of the Partial Major Capsid Protein

The small single-stranded DNA (ssDNA) bacteriophages of the subfamily Gokushovirinae were traditionally perceived as narrowly targeted, niche-specific viruses infecting obligate parasitic bacteria, such as Chlamydia. The advent of metagenomics revealed gokushoviruses to be widespread in global environmental samples. This study expands knowledge of gokushovirus diversity in the environment by developing a degenerate PCR assay to amplify a portion of the major capsid protein (MCP) gene of gokushoviruses. Over 500 amplicons were sequenced from 10 environmental samples (sediments, sewage, seawater and freshwater), revealing the ubiquity and high diversity of this understudied phage group. Residue-level conservation data generated from multiple alignments was combined with a predicted 3D structure, revealing a tendency for structurally internal residues to be more highly conserved than surface-presenting protein–protein or viral–host interaction domains. Aggregating this data set into a phylogenetic framework, many gokushovirus MCP clades contained samples from multiple environments, although distinct clades dominated the different samples. Antarctic sediment samples contained the most diverse gokushovirus communities, whereas freshwater springs from Florida were the least diverse. Whether the observed diversity is being driven by environmental factors or host-binding interactions remains an open question. The high environmental diversity of this previously overlooked ssDNA viral group necessitates further research elucidating their natural hosts and exploring their ecological roles