Phagocytic activity of peripheral blood monocytes under <i>in vivo</i> and <i>in vitro</i> hypoxia conditions in tolerant and susceptible to oxygen deficiency rats

It is known that there are individual differences in resistance to hypoxia, which can determine the predisposition to the development and severity of various diseases, including infectious, inflammatory and tumor. There are no standardized methods for assessing resistance to hypoxia in experimental animals and humans without hypoxic exposure. The search for molecular-biological markers, identifying people with different resistance to oxygen deficiency under normoxic conditions or under moderate hypoxic exposure is undoubtedly efficient. It is possible that the assessment of the basic resistance to hypoxia can help to predict the development and severity of the course of diseases, the mechanisms of which are associated with oxygen deficiency. One of the methods to assess organism resistance to hypoxia without exposure in a decompression chamber or in highland conditions can be modeling hypoxia in vitro. The aim of the study was to characterize the phagocytic activity of peripheral blood monocytes in tolerant and susceptible to hypoxia Wistar rats under normoxic conditions, as well as after hypoxic exposure in vitro and in vivo. The resistance of rats to hypoxia was determined by the gasping time at an altitude of 11.500 m in a decompression chamber. A month after determining the resistance to hypoxia, one group of rats was placed in a decompression chamber at an altitude of 5,000 m for 1 hour to simulate the hypoxic state in vivo. Blood from the tail vein of the other group of rats was placed in 1% oxygen for 1 hour to simulate the hypoxic state in vitro. The phagocytic activity of peripheral blood monocytes was assessed by flow cytometry. It was demonstrated that phagocytic activity of monocytes did not differ in tolerant and susceptible to hypoxia rats under normoxic conditions. The phagocytic activity of monocytes after in vitro and in vivo hypoxic exposure was higher in tolerant to hypoxia animals in comparison to susceptible ones. An increase in the phagocytic activity of monocytes compared to normoxia conditions was observed only in tolerant rats under in vitro conditions of hypoxic exposure. The obtained results indicate that tolerant and susceptible to hypoxia organisms differ in the phagocytic activity of monocytes under conditions of oxygen deficiency, which can determine the course of inflammatory and tumor diseases. The data obtained will be the basis for further experimental investigations organism hypoxia resistance markers

Photometric observations of the supernova 2009nr

We present the results of our UBVRI CCD photometry for the second brightest supernova of 2009, SN 2009nr, discovered during a sky survey with the telescopes of the MASTER robotic network. Its light and color curves and bolometric light curves have been constructed. The light-curve parameters and the maximum luminosity have been determined. SN 2009nr is shown to be similar in light-curve shape and maximum luminosity to SN 1991T, which is the prototype of the class of supernovae Ia with an enhanced luminosity. SN 2009nr exploded far from the center of the spiral galaxy UGC 8255 and most likely belongs to its old halo population. We hypothesize that this explosion is a consequence of the merger of white dwarfs

Magnetic resonances in EuSn2_2As2_2 single crystal

In this work, we report the broad-band ferromagnetic resonance spectroscopy of EuSn$_2$As$_2$ single crystals at different temperatures in combination with magnetization measurements and structural characterization. We observe conventional collective acoustic resonance mode of the A-type antiferromagnetic spin-flop phase in the Eu sub-lattice, and its transition to the paramagnetic resonance above the ordering temperature. Furthermore, we observe reproducibly additional well-defined spectral line. The origin of the additional line remains unclear. However, its temperature dependence attributes it to magnetism in the Eu sub-lattice.Comment: 6 pages, 4 figures, 34 reference

Диагностические маркеры стеатоза печени у больных хроническим вирусным гепатитом C

Objective was to conduct a comparative analysis of routine clinical and laboratory parameters in two groups of patients with chronic viral hepatitis C (CHC) depending on the presence or absence of hepatic steatosis.Methods and materials. The observational retrospective cross-sectional study included 411 patients with CHC, of which 43.80 % were those with hepatic steatosis. The studied parameters were routine clinical and laboratory parameters, the viral genotype, as well as formalized results of a liver biopsy and ultrasound examination of the abdominal organs. To assess the differences between the two groups, the following were calculated: Pearson’s test, two-tailed Fisher’s test, Wilcoxon rank sum test, Spearman’s correlation coefficient, and partial correlation coefficient.Results. It was found that CHC patients with hepatic steatosis were older and had a higher body mass index (BMI) than patients without hepatic steatosis (p&lt;0.01). Among patients with hepatic steatosis, the 3a genotype of the virus (p&lt;0.001) and advanced stages of liver fibrosis (p&lt;0.01) were more often detected, higher concentrations of hepatic transaminase (p£0.001) and iron (p&lt;0 .01) in blood serum were observed, as well as higher liver (p&lt;0.001) and pancreas (p&lt;0.001) sizes on ultrasound than among patients without hepatic steatosis.Conclusion. Age, BMI, viral genotype, stage of liver fibrosis, ALT, AST, GGT, and iron levels, as well as liver and pancreas size are potential predictors of the presence of hepatic steatosis in CHC patients.Цель – провести сравнительный анализ рутинных клинико-лабораторных показателей в 2 группах больных хроническим вирусным гепатитом C (ХГС) в зависимости от наличия или отсутствия стеатоза печени.Методы и материалы. В обсервационное ретроспективное поперечное исследование было включено 411 больных ХГС, из них лиц со стеатозом печени – 43,80 %. Изучаемыми показателями служили рутинные клинико-лабораторные параметры, генотип вируса, а также формализованные результаты биопсии печени и ультразвукового исследования (УЗИ) органов брюшной полости. Для оценки различий между 2 группами рассчитывались критерий согласия Пирсона, двусторонний точный критерий Фишера, W-критерий суммы рангов Уилкоксона, коэффициент корреляции Спирмена, а также коэффициент частной корреляции.Результаты. Установлено, что больные ХГС со стеатозом печени были старше и имели более высокий индекс массы тела (ИМТ), чем больные без стеатоза печени (p&lt;0,01). Среди пациентов со стеатозом печени чаще выявлялся 3a генотип вируса (p&lt;0,001), продвинутые стадии фиброза печени (p&lt;0,01), наблюдались более высокая активность печеночных трансаминаз (p&lt;= 0,001) и концентрация железа (p&lt;0,01) в сыворотке крови, а также более высокие значения размеров печени (p&lt;0,001) и поджелудочной железы (p&lt;0,001) при УЗИ, чем среди пациентов без стеатоза печени.Заключение. Возраст, ИМТ, генотип вируса, стадия фиброза печени, активность АЛТ, АСТ, ГГТ и концентрация железа, а также размеры печени и поджелудочной железы являются потенциальными предикторами наличия стеатоза печени у больных ХГС

Screeing of Patients with Essential Hypertension as a Prevention Method Hypertensive Nephropathy

Purpose: To determine prevalence and evaluate the risk of hypertensive nephropathy in patients with hypertension.Materials and Methods: Based on the city polyclinic № 16 Rostov-on-Don and the Center for Restorative Medicine and Rehabilitation, the mines was carried out screening of patients with hypertension. The study included 330 hypertensive patients without chronic kidney disease in history, of which 303 (91.8%) patients were male, 27 (8.2%) patients – women.Results: It was found that not all clinical and laboratory parameters are affecting the risk of kidney damage in patients with high blood pressure.Summary: Hypertension leads to irreversible renal disorders, assessment of risk is an integral part of the MT treatment activities conducted in these patients

Luminescent properties of Bi-doped polycrystalline KAlCl4

We observed an intensive near-infrared luminescence in Bi-doped KAlCl4 polycrystalline material. Luminescence dependence on the excitation wavelength and temperature of the sample was studied. Our experimental results allow asserting that the luminescence peaked near 1 um belongs solely to Bi+ ion which isomorphically substitutes potassium in the crystal. It was also demonstrated that Bi+ luminescence features strongly depend on the local ion surroundings