¹²⁵I-9MPA signals and correlation with ^99mTc-MIBI signals.

<p>(A) In DS rats fed an HS diet (n = 26), the signals for ¹²⁵I-9MPA, an indicator of the activity of mitochondrial β-oxidation, decreased compared with those in DS rats fed an LS diet (n = 10). *<i>P</i> < 0.05 versus LS. (B) ¹²⁵I-9MPA signals positively correlated with ^99mTc-MIBI signals (r = 0.74, <i>P</i> < 0.0001, n = 36).</p

Postmortem analysis of Dahl rats at 18 weeks old.

<p>Values are expressed as the mean ± SEM.</p><p>*P < 0.05 versus LS at the same time settings.</p><p>LS, rats fed a low-salt diet; HS, rats fed a high-salt diet;</p><p>Postmortem analysis of Dahl rats at 18 weeks old.</p

Body weight, pulse rate and blood pressure of 11 week-old DS rats.

<p>Values are expressed as the mean ± SEM.</p><p>*<i>P</i> < 0.05 versus LS.</p><p><i>DS rats</i>, Dahl salt-sensitive rats; <i>LS</i>, rats fed a low-salt diet; <i>HS</i>, rats fed a high-salt diet;</p><p>SEM, standard error of the mean.</p><p>Body weight, pulse rate and blood pressure of 11 week-old DS rats.</p

^99mTc-MIBI signals were decreased in the hearts of DS rats fed a high-salt diet.

<p>(A) A schematic representation of the experiments used to analyze ^99mTc-MIBI and ¹²⁵I-9MPA signals in DS rats. Analyses of ^99mTc-MIBI and ¹²⁵I-9MPA signals in DS rats were performed at 45 min and 6h after injection. (B) At 45 min after injection, the ^99mTc-MIBI signals were lower in DS rats fed an HS diet than those in DS rats fed an LS diet. In the DS rat fed an HS diet and an LS diet, ^99mTc-MIBI signal per heart tissue weight decreased 6 h after the injection compared to 45 min after the injection (left panel) after correction for physical decay. In DS rats fed an HS diet with heart failure at 18 weeks of age, ^99mTc-MIBI signal per heart tissue weight decreased compared to those in control <b> </b>rats fed an LS diet at 6 h after injection. Signals for ^99mTc-MIBI in the liver (middle panel) or blood (right panel) were low in both LS and HS group at 6 h after injection. (LS, 45 min: n = 10, 6 h: n = 10; HS, 45 min: n = 26, 6h: n = 26). LS, low-salt; HS, high-salt. *<i>P</i> < 0.05 versus LS. †<i>P</i> < 0.05 versus the early phase group.</p

Correlation between ^99mTc-MIBI signals and heart weight.

<p>(A) <b> </b>^99mTc-MIBI signal per gram of heart tissue 45 min after the injection was inversely correlated with heart weight (r = -0.88, n = 35, <i>P</i> < 0.0001). (B) ^99mTc-MIBI signal per gram of tissue 6 h after the injection inversely correlated with heart weight (r = -0.81, n = 36, <i>P</i> < 0.0001).</p

^99mTc-MIBI signals were decreased in the hearts of rats administered CCCP.

<p>(A) A schematic of the experiment to investigate whether CCCP decreased the ^99mTc-MIBI signals in rats. (B) At 180 min after ^99mTc-MIBI injection, the ^99mTc-MIBI signals of the hearts in the CCCP group were significantly lower than those in the rats administered vehicle (n = 7 in each group). *<i>P</i> < 0.05 versus vehicle.</p

Primer sequences used in real time quantitative RT-PCR.

<p>Primer sequences used in real time quantitative RT-PCR.</p

CCCP decreased TMRE signals in <i>ex vivo</i> perfused hearts.

<p>(A) Schematic of the experiment. Hearts were perfused with TMRE, and the signals were observed using two-photon laser microscopy. The images were obtained serially from hearts perfused with buffer containing vehicle or CCCP (0.1 or 1 μM). (B) Representative images of TMRE signals. Scale bar: 50μm. (C) CCCP decreased the TMRE signals of perfused hearts in a dose-dependent manner 20 min after CCCP perfusion (n = 3–4 per group). *<i>P</i> < 0.05 versus vehicle.</p

Phosphocreatine and ATP were decreased in the hearts CCCP-administered rats.

<p>(A) Representative images of <i>in situ</i> cardiac ³¹P magnetic resonance spectra. ppm, parts per million. (B) The rats administered CCCP showed decreased PCr and βATP (Vehicle: n = 4, CCCP: n = 5). *<i>P</i> < 0.05 versus vehicle-administered rats.</p

Correlation between ^99mTc-MIBI signals and cardiac function.

<p>(A, B) Correlation between fractional shortening on echocardiography and ^99mTc-MIBI signals 45 min after the injection (r = -0.70, n = 36, <i>P</i> = 0.015; A) or 6 h after the injection (r = -0.57, n = 36, <i>P</i> = 0.037; B). (C) Atrial natriuretic factor (<i>ANF)</i> gene expression was significantly increased in DS rats fed an HS diet (HS; n = 52) compared to DS rats fed an LS diet (LS; n = 20). *<i>P</i> < 0.05 versus LS. (D, E) Correlations between ANF gene expression and ^99mTc-MIBI signals 45 min (r = -0.56, n = 36, <i>P</i> = 0.0003; D) and 6 h (r = -0.68, n = 36, <i>P</i> < 0.00001; E) after the injection.</p