Dose-response curve of the effect of RBC hemolysate (% ^v/_v in medium) on ds-DNA ratio in H₂O₂-stimulated PBMC.

<p>p<0.001 for the effects of the 0.3, 0.6, 1 and 2% ^v/_v RBC hemolysate concentrations.</p

Dose-response curve of the effect of RBC hemolysate (% ^v/_v in medium) on H₂O₂-induced DNA repair (% of control) in PBMC.

<p>For the effects of 0.3, 0.6, 1 and 2% ^v/_v hemolysate, p = NS, p = NS, p<0.005 and p<0.005, respectively.</p

Correlation between ds-DNA ratio (control = 1.00) and DNA repair (% of control) in H₂O₂-stimulated PBMC, at concentrations of 0, 0.3, 0.6, 1 and 2% ^v/_v RBC hemolysate.

<p>For each point, N = 8. Coefficient r = −0.925, p<0.025. Regression line: y = −0.131x +2.432.</p

Dose response curve of the effect of H₂O₂ on ds DNA.

<p>Effect of 10, 20, 50, 100 and 200 µmol/L H₂O₂ on PBMC was tested: 100 and 200 µmol/L reduced %ds DNA significantly (p<0.02, p<0.01, respectively).</p

ds-DNA ratio in H₂O₂-stimulated PBMC: effect of purchased human hemoglobin (1.8 mg/ml) and RBC hemolysate (2% ^v/_v, mean hemoglobin content = 2.00±0.07 mg/ml).

<p>b vs a, p<0.001; c vs a, p<0.001; N = 8.</p

Comet assay – a representative experiment (N = 3).

<p>The left image depicts the control. The middle image depicts the effect of H₂O₂. The right image depicts the effect of H₂O₂ and RBC hemolysate.</p