Rabies virus strains circulating in Bhutan: implications for control.

postprintWe report a molecular epidemiological study of rabies virus strains circulating in animal populations in Bhutan, and investigate potential origins of these viruses. Twenty-three rabies virus isolates originating from dogs and other domestic animals were characterized by sequencing the partial nucleoprotein (N) gene (395 bp). Phylogenetic analysis was conducted and the Bhutanese isolates were compared with rabies viruses originating from other parts of the world. Phylogenetic analysis showed that Bhutanese isolates were highly similar and were closely related to Indian strains and South Asian Arctic–like–1 viruses. Our study suggests that the rabies viruses spreading in southern parts of Bhutan have originated from a common ancestor, perhaps from the Indian virus strain. Keywords: molecular epidemiology, rabies, Arctic–like virus, BhutanThailand Research Fund (grant No.DBG5180026

Rabies virus strains circulating in Bhutan: implications for control.

We report a molecular epidemiological study of rabies virus strains circulating in animal populations in Bhutan, and investigate potential origins of these viruses. Twenty-three rabies virus isolates originating from dogs and other domestic animals were characterized by sequencing the partial nucleoprotein (N) gene (395 bp). Phylogenetic analysis was conducted and the Bhutanese isolates were compared with rabies viruses originating from other parts of the world. Phylogenetic analysis showed that Bhutanese isolates were highly similar and were closely related to Indian strains and South Asian Arctic–like–1 viruses. Our study suggests that the rabies viruses spreading in southern parts of Bhutan have originated from a common ancestor, perhaps from the Indian virus strain. Keywords: molecular epidemiology, rabies, Arctic–like virus, BhutanThailand Research Fund (grant No.DBG5180026

The potential of digital tools to foster production, and thus availability, of healthy diets for city dwellers in secondary cities

Urban population growth and strong rural-urban migration are continuing in sub-Saharan Africa. Similarly, traditional farming is no longer attractive to many young people despite its importance for food security. Digitalization can be an ally to make the agri-food system more attractive. The Nutrition in City Ecosystems (NICE) project aims to increase and better link the production and demand of safe and healthy foods in two secondary cities each in Bangladesh, Kenya and Rwanda. Two digital tools called Soluta-ag and GATE are introduced in NICE. Soluta-ag keeps records of business transactions in online and offline mode and provides marketplace facilities and automated business analytics in real-time for Farmers’ Hubs, the social business model implemented in NICE. GATE, on the other hand, serves as a selection, screening and validation platform as well as repository for climate smart agri-food system innovations. Preliminary experiences from NICE show that digital tools can A) attract youth for farming, B) connect farmers’ produce to market, and C) facilitate the delivery of contextadapted innovations. Ensuring ways of production are modern and efficient and sufficient employment opportunities are available in urban agri-food systems are crucial to maintain food sovereignty and adequate, diversified nutrition for all city dwellers

Yield of Brain MRI in Clinically Diagnosed Epilepsy in the Kingdom of Bhutan: A Prospective Study

Background: People with epilepsy (PWE) in low- and middle-income countries may not access the health resources that are considered optimal for epilepsy diagnosis. The diagnostic yield of magnetic resonance imaging (MRI) has not been well studied in these settings. Objectives: To report the diagnostic yield of brain MRI and identify clinical associations of abnormal MRI findings among PWE in a neurocysticercosis-endemic, resource-limited setting and to identify the proportion and putative structural brain causes of drug-resistant epilepsy. Methods: PWE were prospectively enrolled at the Jigme Dorji Wangchuck National Referral Hospital in Bhutan (2014-2015). Each participant completed clinical questionnaires and a 1.5-Tesla brain MRI. Each MRI was reviewed by at least 1 radiologist and neurologist in Bhutan and the United States. A working definition of drug-resistant epilepsy for resource-limited settings was given as (a) seizures for >1 year, (b) at least 1 seizure in the prior year, and (c) presently taking 2 or more antiepileptic drugs (AEDs). Logistic regression models were constructed to test the cross-sectional association of an abnormal brain MRI with clinical variables. Findings: A total of 217 participants (125 [57%] female; 54 [25%] neurocysticercosis (n = 26, 12%, including 1 child) and congenital/perinatal abnormalities (n = 29, 14%, including 14 children). The number of AEDs (odds ratio = .59, 'P' = .03) and duration of epilepsy (odds ratio = 1.11, 'P' = .02) were significantly associated with an abnormal MRI. Seizure in the prior month was associated with the presence of mesial temporal sclerosis (odds ratio = .47, 'P' = .01). A total of 25 (12%) participants met our definition of drug-resistant epilepsy, with mesial temporal sclerosis (n = 10), congenital malformations (n = 5), and neurocysticercosis (n = 4) being the more common findings. Conclusions: The prevalence of abnormalities on brain MRI for PWE in resource-limited settings is high as a result of a diffuse range of etiologies, most commonly mesial temporal sclerosis. Drug-resistant epilepsy accounted for 12% of the referral population in a conservative estimation

Timescapes of Himalayan hydropower: promises, project life cycles, and precarities

In this paper, we review the existing social science scholarship focused on hydropower development in the Himalayan region, using an interpretive lens attuned to issues of time and temporality. While the spatial politics of Himalayan hydropower are well examined in the literature, an explicit examination of temporal politics is lacking. In this paper, we present a conceptual framework organized around the heuristic of timescapes, highlighting temporal themes implicit in the existing literature. In three sections, we explore the temporal politics of anticipation that shape hydropower dreams, the intersecting temporalities and rhythms that modulate the life cycles of hydropower projects, and the ways that geological and hydrological time affect both hydropower development and broader Himalayan futures. Along the way, we pose a series of questions useful for framing future research given the significant climatic, geophysical, and sociopolitical changes underway in the Himalayan bioregion, calling for greater analytical attention to time, temporality, and temporal ethics in future studies of hydropower in the Himalayas and beyond.Austin Lord, Georgina Drew, Mabel Denzin Gerga

Sharing Experience of Infection Control in NICU in Bhutan

The neonatal mortality rate (NMR) in Bhutan has remained one of the highest in Asian countries. To achieve the Millennium Development Goal (MDG) 4 on child mortality (Two thirds reduction from 1990 to 2015), it is essential to reduce NMR. Royal Government of Bhutan is trying to establish neonatal care units in each Comprehensive Emergency Obstetric Care (EmOC) Centers to achieve this goal. Jigme Dorji Wangchuck National Referral Hospital (JDWNRH) is currently the only tertiary care center and the only health facility, which has neonatal ward and Neonatal Intensive Care Unit (NICU) in the country. The author is the first Neonatologist to work in this country as a full time volunteer. This article is to share the author's experience and challenges faced regarding infection control in managing neonatal service in a resource-limited country. Nosocomial transmission of drug-resistant pathogens was intense at NICU in Bhutan; transmission involved mostly drug-resistant gram-negative bacilli. The growing burden of neonatal mortality associated with hospital-acquired neonatal sepsis in the developing world creates an urgent need for cost-effective infection-control measures in resource-limited settings. Infection-control interventions are feasible and are possibly effective in resource-limited hospital settings

Effect of curing methods on quality and drying characteristics of turmeric: Effect of curing methods on quality of turmeric

Turmeric rhizomes of Suranjana cultivar were cured by traditional and microwave methods in water and sodium bicarbonate solution and dried in hot air dryer. Effect of curing methods on moisture content, hardness, colour, curcumin content, essential oil content, total phenolic content and drying rate were investigated. Curing methods did not have significant effect on moisture content of cured rhizomes. Hardness of turmeric rhizomes decreased as curing duration increased. Microwave curing in sodium bicarbonate solution for 6 min resulted in maximum value of the colour indices, highest curcumin content (5.20%) and essential oil content (4.38%) in turmeric powder and highest dry recovery (24.20%). Curing caused significant reduction in drying time. Minimum drying time (20.84 h) was required in microwave curing in water for 8 min, whereas, uncured samples took longest time (29 h) for drying. Drying of all the turmeric rhizomes cured by different methods occurred in falling rate period