Development of the Digital System of Communications and Management in the Oil and Gas Industry

Многие компании в нефтяной отрасли не располагают адекватной цифровой системой коммуникаций и управления. Согласованность целей, наличие эффективных каналов коммуникации и обмена информацией между подрядчиком и заказчиком, позволяет снизить риск возникновения конфликтных ситуаций. Создание единой цифровой площадки, способной осуществлять совместное планирование ресурсов и контроля сроков и задач, становится закономерным шагом в управлении взаимодействием заказчика и исполнителяMany companies in the oil industry do not have an adequate digital communications and management system in place. Consistency of goals, the availability of effective channels of communication and information exchange between the contractor and the customer, can reduce the risk of conflict situations. The creation of a single digital platform capable of joint resource planning and control of deadlines and tasks is becoming a natural step in managing the interaction between the customer and the contracto

Intracellular temperature mapping with a fluorescent polymeric thermometer and fluorescence lifetime imaging microscopy

Cellular functions are fundamentally regulated by intracellular temperature, which influences biochemical reactions inside a cell. Despite the important contributions to biological and medical applications that it would offer, intracellular temperature mapping has not been achieved. Here we demonstrate the first intracellular temperature mapping based on a fluorescent polymeric thermometer and fluorescence lifetime imaging microscopy. The spatial and temperature resolutions of our thermometry were at the diffraction limited level (200 nm) and 0.18–0.58 °C. The intracellular temperature distribution we observed indicated that the nucleus and centrosome of a COS7 cell, both showed a significantly higher temperature than the cytoplasm and that the temperature gap between the nucleus and the cytoplasm differed depending on the cell cycle. The heat production from mitochondria was also observed as a proximal local temperature increase. These results showed that our new intracellular thermometry could determine an intrinsic relationship between the temperature and organelle function

Microscopic Detection of Thermogenesis in a Single HeLa Cell

AbstractWe report here the technique for detection and measurement of the temperature changes in single cells using a recently devised microthermometer (a glass micropipette filled with the thermosensitive fluorescent dye Europium (III) thenoyltrifluoroacetonate trihydrate). We found that the heat production in a single HeLa cell occurred with some time delay after the ionomycin-induced Ca2+ influx from the extracellular space. The time delay inversely depended on extracellular [Ca2+], and the increase in temperature was suppressed when Ca2+-ATPases were blocked by thapsigargin. These observations strongly suggest that the enzymatic activity of Ca2+-ATPases in endoplasmic reticulum leads to the heat production. This study has therefore paved the way for studying the thermogenesis at the single-cell level