Oral biofilm models for mechanical plaque removal

In vitro plaque removal studies require biofilm models that resemble in vivo dental plaque. Here, we compare contact and non-contact removal of single and dual-species biofilms as well as of biofilms grown from human whole saliva in vitro using different biofilm models. Bacteria were adhered to a salivary pellicle for 2 h or grown after adhesion for 16 h, after which, their removal was evaluated. In a contact mode, no differences were observed between the manual, rotating, or sonic brushing; and removal was on average 39%, 84%, and 95% for Streptococcus mutans, Streptococcus oralis, and Actinomyces naeslundii, respectively, and 90% and 54% for the dual- and multi-species biofilms, respectively. However, in a non-contact mode, rotating and sonic brushes still removed considerable numbers of bacteria (24–40%), while the manual brush as a control (5–11%) did not. Single A. naeslundii and dual-species (A. naeslundii and S. oralis) biofilms were more difficult to remove after 16 h growth than after 2 h adhesion (on average, 62% and 93% for 16- and 2-h-old biofilms, respectively), while in contrast, biofilms grown from whole saliva were easier to remove (97% after 16 h and 54% after 2 h of growth). Considering the strong adhesion of dual-species biofilms and their easier more reproducible growth compared with biofilms grown from whole saliva, dual-species biofilms of A. naeslundii and S. oralis are suggested to be preferred for use in mechanical plaque removal studies in vitro

Guided bone regeneration around an immediate transmucosal implant for single tooth replacement: a case report.

This case report describes the procedure of guided bone regeneration around an endosseous dental implant, placed into an extraction alveolus in the absence of total soft tissue coverage, ie, in a transmucosal condition. The technique presented permits an immediate placement of the implant after the extraction of the maxillary right lateral incisor. A nonresorbable barrier membrane was used, and strict chemical plaque control was maintained during osseointegration. After an uneventful healing period of 6 months, the membrane was removed in a second-stage surgery. Two months later, the fixed single tooth prosthesis was placed. The learning objective of this article is to explain the immediate transmucosal implant surgery technique, using a case report to illustrate the procedure.link_to_subscribed_fulltex

Case report. Direct transmucosal implants (I): the immediate treatment of the individual tooth | Kasuistik. Transmukosale Direktimplantate (I): Einzelzahnsofortversorgung.

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