Temporal variation of bacterial community and nutrients in Tibetan glacier snowpack

The Tibetan Plateau harbors the largest number of glaciers outside the polar regions, which are the source of several major rivers in Asia. These glaciers are also major sources of nutrients for downstream ecosystems, while there is a little amount of data available on the nutrient transformation processes on the glacier surface. Here, we monitored the carbon and nitrogen concentration changes in a snowpit following a snowfall in the Dunde Glacier of the Tibetan Plateau. The association of carbon and nitrogen changes with bacterial community dynamics was investigated in the surface and subsurface snow (depth at 0–15 and 15–30 cm, respectively) during a 9 d period. Our results revealed rapid temporal changes in nitrogen (including nitrate and ammonium) and bacterial communities in both surface and subsurface snow. Nitrate and ammonium concentrations increased from 0.44 to 1.15 mg L−1 and 0.18 to 0.24 mg L−1 in the surface snow and decreased from 3.81 to 1.04 and 0.53 to 0.25 mg L−1 in the subsurface snow over time. Therefore, we suggest that the surface snow is not nitrogen-limited, while the subsurface snow is associated with nitrogen consumption processes and is nitrogen-limited. The nitrate concentration co-varied with bacterial diversity, community structure, and the predicted nitrogen fixation and nitrogen assimilation/denitrification-related genes (narG), suggesting nitrogen could mediate bacterial community changes. The nitrogen limitation and enriched denitrification-related genes in subsurface snow suggested stronger environmental and biotic filtering than those in surface snow, which may explain the lower bacterial diversity, more pronounced community temporal changes, and stronger biotic interactions. Collectively, these findings advance our understanding of bacterial community variations and bacterial interactions after snow deposition and provide a possible biological explanation for nitrogen dynamics in snow

Scientific access into Mercer Subglacial Lake: Scientific objectives, drilling operations and initial observations

The Subglacial Antarctic Lakes Scientific Access (SALSA) Project accessed Mercer Subglacial Lake using environmentally clean hot-water drilling to examine interactions among ice, water, sediment, rock, microbes and carbon reservoirs within the lake water column and underlying sediments. A ∼0.4 m diameter borehole was melted through 1087 m of ice and maintained over ∼10 days, allowing observation of ice properties and collection of water and sediment with various tools. Over this period, SALSA collected: 60 L of lake water and 10 L of deep borehole water; microbes \u3e0.2 μm in diameter from in situ filtration of ∼100 L of lake water; 10 multicores 0.32-0.49 m long; 1.0 and 1.76 m long gravity cores; three conductivity-temperature-depth profiles of borehole and lake water; five discrete depth current meter measurements in the lake and images of ice, the lake water-ice interface and lake sediments. Temperature and conductivity data showed the hydrodynamic character of water mixing between the borehole and lake after entry. Models simulating melting of the ∼6 m thick basal accreted ice layer imply that debris fall-out through the ∼15 m water column to the lake sediments from borehole melting had little effect on the stratigraphy of surficial sediment cores

Physiological Ecology of Microorgansisms in Subglacial Lake Whillans

Subglacial microbial habitats are widespread in glaciated regions of our planet. Some of these environments have been isolated from the atmosphere and from sunlight for many thousands of years. Consequently, ecosystem processes must rely on energy gained from the oxidation of inorganic substrates or detrital organic matter. Subglacial Lake Whillans (SLW) is one of more than 400 subglacial lakes known to exist under the Antarctic ice sheet; however, little is known about microbial physiology and energetics in these systems. When it was sampled through its 800 m thick ice cover in 2013, the SLW water column was shallow (~2 m deep), oxygenated, and possessed sufficient concentrations of C, N, and P substrates to support microbial growth. Here, we use a combination of physiological assays and models to assess the energetics of microbial life in SLW. In general, SLW microorganisms grew slowly in this energy-limited environment. Heterotrophic cellular carbon turnover times, calculated from 3H-thymidine and 3H-leucine incorporation rates, were long (60 to 500 days) while cellular doubling times averaged 196 days. Inferred growth rates (average ~0.006 d-1) obtained from the same incubations were at least an order of magnitude lower than those measured in Antarctic surface lakes and oligotrophic areas of the ocean. Low growth efficiency (8%) indicated that heterotrophic populations in SLW partition a majority of their carbon demand to cellular maintenance rather than growth. Chemoautotrophic CO2-fixation exceeded heterotrophic organic C-demand by a factor of ~1.5. Aerobic respiratory activity associated with heterotrophic and chemoautotrophic metabolism surpassed the estimated supply of oxygen to SLW, implying that microbial activity could deplete the oxygenated waters, resulting in anoxia. We used thermodynamic calculations to examine the biogeochemical and energetic consequences of environmentally imposed switching between aerobic and anaerobic metabolisms in the SLW water column. Heterotrophic metabolisms utilizing acetate and formate as electron donors yielded less energy than chemolithotrophic metabolisms when calculated in terms of energy density, which supports experimental results that showed chemoautotrophic activity in excess of heterotrophic activity. The microbial communities of subglacial lake ecosystems provide important natural laboratories to study the physiological and biogeochemical behavior of microorganisms inhabiting cold, dark environments

Biogeochemical and historical drivers of microbial community composition and structure in sediments from Mercer Subglacial Lake, West Antarctica

Ice streams that flow into Ross Ice Shelf are underlain by water-saturated sediments, a dynamic hydrological system, and subglacial lakes that intermittently discharge water downstream across grounding zones of West Antarctic Ice Sheet (WAIS). A 2.06 m composite sediment profile was recently recovered from Mercer Subglacial Lake, a 15 m deep water cavity beneath a 1087 m thick portion of the Mercer Ice Stream. We examined microbial abundances, used 16S rRNA gene amplicon sequencing to assess community structures, and characterized extracellular polymeric substances (EPS) associated with distinct lithologic units in the sediments. Bacterial and archaeal communities in the surficial sediments are more abundant and diverse, with significantly different compositions from those found deeper in the sediment column. The most abundant taxa are related to chemolithoautotrophs capable of oxidizing reduced nitrogen, sulfur, and iron compounds with oxygen, nitrate, or iron. Concentrations of dissolved methane and total organic carbon together with water content in the sediments are the strongest predictors of taxon and community composition. δ¹³C values for EPS (−25 to −30‰) are consistent with the primary source of carbon for biosynthesis originating from legacy marine organic matter. Comparison of communities to those in lake sediments under an adjacent ice stream (Whillans Subglacial Lake) and near its grounding zone provide seminal evidence for a subglacial metacommunity that is biogeochemically and evolutionarily linked through ice sheet dynamics and the transport of microbes, water, and sediments beneath WAIS

Solute sources and geochemical processes in Subglacial Lake Whillans, West Antarctica

Subglacial Lake Whillans (SLW), West Antarctica, is an active component of the subglacial hydrological network located beneath 800 m of ice. The fill and drain behavior of SLW leads to long (years to decades) water residence times relative to those in mountain glacier systems. Here, we present the aqueous geochemistry of the SLW water column and pore waters from a 38-cm-long sediment core. Stable isotopes indicate that the water is primarily sourced from basal-ice melt with a minor contribution from seawater that reaches a maximum of ~6% in pore water at the bottom of the sediment core. Silicate weathering products dominate the crustal (non-seawater) component of lake- and pore-water solutes, and there is evidence for cation exchange processes within the clay-rich lake sediments. The crustal solute component ranges from 6 meq L -1 in lake waters to 17 meq L -1 in the deepest pore waters. The porewater profiles of the major dissolved ions indicate a more concentrated solute source at depth (>38 cm). The combination of significant seawater and crustal components to SLW lake and sediment pore waters in concert with ion exchange processes result in a weathering regime that contrasts with other subglacial systems. The results also indicate cycling of marine water sourced from the sediments back to the ocean during lake drainage events

A decade of shaping the futures of polar early career researchers: A legacy of the International Polar Year

The Association of Polar Early Career Scientists (APECS) is an important legacy of the International Polar Year (IPY). APECS continues to foster engagement in education, outreach and communication (EOC) activities relating to the polar regions and provide training for early career researchers (ECRs). We highlight opportunities for training, leadership and skills development, such as the annual Polar Weeks and Antarctica Day celebrations. Participation and engagement in EOC activities actively contributes to career development by enabling ECRs to develop valuable soft skills such as networking, communication and interdisciplinary knowledge. A pilot survey on EOC engagement highlighted that those who organise events also gain leadership skills such as team management. We discuss several factors contributing to the success of APECS in training the next generation of polar leaders. These include the geographical rather than discipline-specific focus of the organisation, utilisation of online resources, including social media, and the strong links with partner organisations. These examples demonstrate how the EOC legacy of IPY has continued due to APECS’ targeted efforts to create EOC opportunities and provide skills and leadership training for ECRs

Biogeochemical and historical drivers of microbial community composition and structure in sediments from Mercer Subglacial Lake, West Antarctica

Ice streams that flow into Ross Ice Shelf are underlain by water-saturated sediments, a dynamic hydrological system, and subglacial lakes that intermittently discharge water downstream across grounding zones of West Antarctic Ice Sheet (WAIS). A 2.06 m composite sediment profile was recently recovered from Mercer Subglacial Lake, a 15 m deep water cavity beneath a 1087 m thick portion of the Mercer Ice Stream. We examined microbial abundances, used 16S rRNA gene amplicon sequencing to assess community structures, and characterized extracellular polymeric substances (EPS) associated with distinct lithologic units in the sediments. Bacterial and archaeal communities in the surficial sediments are more abundant and diverse, with significantly different compositions from those found deeper in the sediment column. The most abundant taxa are related to chemolithoautotrophs capable of oxidizing reduced nitrogen, sulfur, and iron compounds with oxygen, nitrate, or iron. Concentrations of dissolved methane and total organic carbon together with water content in the sediments are the strongest predictors of taxon and community composition. δ¹³C values for EPS (−25 to −30‰) are consistent with the primary source of carbon for biosynthesis originating from legacy marine organic matter. Comparison of communities to those in lake sediments under an adjacent ice stream (Whillans Subglacial Lake) and near its grounding zone provide seminal evidence for a subglacial metacommunity that is biogeochemically and evolutionarily linked through ice sheet dynamics and the transport of microbes, water, and sediments beneath WAIS

Microbial sulfur transformations in sediments from Subglacial Lake Whillans

Diverse microbial assemblages inhabit subglacial aquatic environments. While few of these environments have been sampled, data reveal that subglacial organisms gain energy for growth from reduced minerals containing nitrogen, iron, and sulfur. Here we investigate the role of microbially mediated sulfur transformations in sediments from Subglacial Lake Whillans (SLW), Antarctica, by examining key genes involved in dissimilatory sulfur oxidation and reduction. The presence of sulfur transformation genes throughout the top 34 cm of SLW sediments changes with depth. SLW surficial sediments were dominated by genes related to known sulfur-oxidizing chemoautotrophs. Sequences encoding the adenosine-5’-phosphosulfate (APS) reductase gene, involved in both dissimilatory sulfate reduction and sulfur oxidation, were present in all samples and clustered into 16 distinct OTUs. The majority of APS reductase sequences (74%) clustered with known sulfur oxidizers including those within the Sideroxydans and Thiobacillus genera. Reverse-acting dissimilatory sulfite reductase (rDSR) and 16S rRNA gene sequences further support dominance of Sideroxydans and Thiobacillus phylotypes in the top 2 cm of SLW sediments. The SLW microbial community has the genetic potential for sulfate reduction which is supported by experimentally measured low rates (1.4 pmol cm-3d-1) of biologically mediated sulfate reduction and the presence of APS reductase and DSR gene sequences related to Desulfobacteraceae and Desulfotomaculum. Our results also infer the presence of sulfur oxidation, which can be a significant energetic pathway for chemosynthetic biosynthesis in SLW sediments. The water in SLW ultimately flows into the Ross Sea where intermediates from subglacial sulfur transformations can influence the flux of solutes to the Southern Ocean