Rapid establishment of the European Bank for induced Pluripotent Stem Cells (EBiSC):The Hot Start experience

A fast track “Hot Start” process was implemented to launch the European Bank for Induced Pluripotent Stem Cells (EBiSC) to provide early release of a range of established control and disease linked human induced pluripotent stem cell (hiPSC) lines. Established practice amongst consortium members was surveyed to arrive at harmonised and publically accessible Standard Operations Procedures (SOPs) for tissue procurement, bio-sample tracking, iPSC expansion, cryopreservation, qualification and distribution to the research community. These were implemented to create a quality managed foundational collection of lines and associated data made available for distribution. Here we report on the successful outcome of this experience and work flow for banking and facilitating access to an otherwise disparate European resource, with lessons to benefit the international research community. eTOC: The report focuses on the EBiSC experience of rapidly establishing an operational capacity to procure, bank and distribute a foundational collection of established hiPSC lines. It validates the feasibility and defines the challenges of harnessing and integrating the capability and productivity of centres across Europe using commonly available resources currently in the field

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Not AvailableThe study has been conducted in the mid Himalayas of Uttarakhand for the availability of water resources for domestic and irrigation usage. The study was conducted in four villages namely Deu, Dhoira, Jutaya and Nichiya of Kalsi block of Dehradun district. From each village, 25 respondents were interviewed for asking details about water resources. The respondents were asked about number of springs which have been dried for last 5 years, the causes for drying of natural resources, impact of spring drying on the livelihood of people and the means of overcoming water shortage, and Govt. Role in Village upliftment. From the results, it was found out that for last 5 years; about 4-5 springs have been dried up. The major causes of drying of natural water resources were reduced rainfall, hot weather and reduction in number of forest trees. The impacts of drying of natural resources were: more time is involved in water collection activity and unavailability of water for irrigation purposes. The means of overcoming water shortages were naularecharge and afforestation etc. The facilities provided by the Govt. Official as reported by the respondents were imparting training, agricultural related activities, loan related facilities and participation in self help group activities etc.Not Availabl

Not Available

Not AvailableThe study has been conducted in the mid Himalayas of Uttarakhand for the availability of water resources for domestic and irrigation usage. The study was conducted in four villages namely Deu, Dhoira, Jutaya and Nichiya of Kalsi block of Dehradun district. From each village, 25 respondents were interviewed for asking details about water resources. The respondents were asked about number of springs which have been dried for last 5 years, the causes for drying of natural resources, impact of spring drying on the livelihood of people and the means of overcoming water shortage, and Govt. Role in Village upliftment. From the results, it was found out that for last 5 years; about 4-5 springs have been dried up. The major causes of drying of natural water resources were reduced rainfall, hot weather and reduction in number of forest trees. The impacts of drying of natural resources were: more time is involved in water collection activity and unavailability of water for irrigation purposes. The means of overcoming water shortages were naula recharge and afforestation etc. The facilities provided by the Govt. Official as reported by the respondents were imparting training, agricultural related activities, loan related facilities and participation in self help group activities etc.Not Availabl