Standardization and quality control of<i> Darvyadi Pravahi Kvatha</i> - An Ayurvedic formulation

654-658The present study deals with the standardization and quality control of the Ayurvedic formulation Darvyadi Pravahi Kvatha following quality control procedures for the finished product. The results of physico-chemical parameters, viz. specific gravity (1.02), total solids (4.83%) and pH (5.54) were found. Microbiological limit test and heavy metals Pb, Cd, As, Hg were also found within the limits set by Ayurvedic Pharmacopiea of India (API). The obtained values can be adapted to lay down new pharmacopoeial standards with batch to batch consistency. The phytochemical constituents found in the raw material used for the preparation of Darvyadi Pravahi Kvatha facilitate the desirable therapeutic efficacy of the medicinal formulations a whole in elements and also could help in understanding the underlying mechanism of pharmacological action

Standardization and quality control of<i> Kutajastaka Kvatha Ghana Vati</i>: An <i>Ayurvedic </i> formulation

310-313Quality assurance of herbal medicine is an important factor and basic requirement for herbal drug industry and other drug development organizations. Kutajastaka Kvatha Ghana Vati is one of the ancient, most commonly used Ayurvedic formulations which are made in combination of nine plant ingredients. Ayurvedic literature reveals that the formulation was used for the treatment of Daha (burning sensation), Raktatisara (Diarrhoea with blood), Sula (pain), Amadosa (products of impaired digestion and metabolism) and Sarvatisara (all types of diarrhoea). Due to the lack of quality standards, there are batch to batch variations. The present study was thus undertaken to develop standards for the quality control of the Vati. All the ingredients were procured locally; identified by a taxonomist and an Ayurvedacharya; Vati was prepared in laboratory scale using GMP; and analyzed as per standard methods. All the samples were subjected to physico-chemical analysis, HPTLC finger printing, heavy metals analysis, and microbial load. The data obtained can be adopted for laying down the pharmacopoeial standards for Kutajastaka Kvatha Ghana Vati

Standardization and quality control of Kutajastaka Kvatha Ghana Vati: An Ayurvedic formulation

Quality assurance of herbal medicine is an important factor and basic requirement for herbal drug industry and other drug development organizations. Kutajastaka Kvatha Ghana Vati is one of the ancient, most commonly used Ayurvedic formulations which are made in combination of nine plant ingredients. Ayurvedic literature reveals that the formulation was used for the treatment of Daha (burning sensation), Raktatisara (Diarrhoea with blood), Sula (pain), Amadosa (products of impaired digestion and metabolism) and Sarvatisara (all types of diarrhoea). Due to the lack of quality standards, there are batch to batch variations. The present study was thus undertaken to develop standards for the quality control of the Vati. All the ingredients were procured locally; identified by a taxonomist and an Ayurvedacharya; Vati was prepared in laboratory scale using GMP; and analyzed as per standard methods. All the samples were subjected to physico-chemical analysis, HPTLC finger printing, heavy metals analysis, and microbial load. The data obtained can be adopted for laying down the pharmacopoeial standards for Kutajastaka Kvatha Ghana Vati

Standardization of <i>Dhanyapanchaka Kvatha Ghana Vati</i>- An <i>Ayurvedic</i> formulation

386-390Quality assurance is an integral part of all systems of medicine to ensure quality medicament. Thus, there is an urgent need to evaluate such parameters which can be adopted by the pharmaceutical industries. In the communication, attempts have been made to standardize Dhanyapanchaka Kvatha Ghana Vati, an Ayurvedic compound formulation. Standardization and quality control of Ayurvedic formulations is necessary to ensure their quality, strength, purity and authenticity. Present work deals with physico-chemical analysis, high performance thin layer chromatography (HPTLC), microbial limit test and heavy metals analysis of Dhanyapanchaka Kvatha Ghana Vati. The outcomes of the research confirm to the need of ensuring quality and safety of Ayurvedic medicines

Association of angiotensin-converting enzyme gene I/D polymorphism with steroid responsiveness in childhood nephrotic syndrome

The aim of the study was to study the distribution of angiotensin-converting enzyme (ACE) gene insertion/deletion (I/D) polymorphism, and its association with steroid responsiveness in children with idiopathic nephrotic syndrome (INS). One hundred twenty-five children with INS were classified into two groups: steroid-sensitive nephrotic syndrome (SSNS: n = 90) and steroid-resistant nephrotic syndrome (SRNS: n=35). The control group consisted of 150 unrelated healthy children. Genomic DNA was extracted from peripheral leucocytes by the standard salting-out method. ACE genotyping was performed and ACE genotypes DD, ID, and II were compared between different groups. The frequency distribution of the DD genotype was significantly increased in children with INS compared to control subjects (P = 0.0012) while the difference was not significant (P = 0.071) between SSNS and control subjects. The frequency distribution of the DD genotype was significantly high in the SRNS group compared to control subjects (P < 0.0001). The distribution of the DD genotype was high in SRNS compared to SSNS group patients (P = 0.016). In conclusion, the presence of the DD genotype may predict risk for steroid resistance in childhood INS