Porcentages phagocytosis’ evaluation uva irradiation effects of haemocytes and glucose’s concentration haemolymph in pink-shrimp Farfantepenaeus paulensis

Submitted by Vanessa Brum da Silva ([email protected]) on 2010-09-29T18:09:59Z No. of bitstreams: 1 AVALIAÇÃO DO EFEITO DA RADIAÇÃO ULTRAVIOLETA “A” SOBRE PORCENTAGENS DE.pdf: 88932 bytes, checksum: 38271bb6551925b5896ecea67f098d08 (MD5)Approved for entry into archive by Helena Moraes([email protected]) on 2010-09-30T22:33:09Z (GMT) No. of bitstreams: 1 AVALIAÇÃO DO EFEITO DA RADIAÇÃO ULTRAVIOLETA “A” SOBRE PORCENTAGENS DE.pdf: 88932 bytes, checksum: 38271bb6551925b5896ecea67f098d08 (MD5)Made available in DSpace on 2010-09-30T22:33:09Z (GMT). No. of bitstreams: 1 AVALIAÇÃO DO EFEITO DA RADIAÇÃO ULTRAVIOLETA “A” SOBRE PORCENTAGENS DE.pdf: 88932 bytes, checksum: 38271bb6551925b5896ecea67f098d08 (MD5) Previous issue date: 2008Os crustáceos estão naturalmente expostos à radiação ultravioleta, podendo esta produzir mudanças bioquímicas e na função imune e predispor ao aparecimento de doenças. Objetivo: Quantificar a concentração de glicose na hemolinfa e avaliar o índice fagocitário em hemócitos de camarões Farfantepenaeus paulensis expostos à radiação UVA. Metodologia: 21 camarões foram divididos em três grupos submetidos à radiação UVA (tratamentos de 1, 5 e 10 dias) e grupo controle. A fagocitose foi efetuada em lâminas, nas quais os hemócitos foram avaliados pela sua capacidade de ingerir leveduras (Sacharomyces cerevisae). A glicose foi determinada por método enzimático (glicose oxidase). Os resultados foram expressos como média ± desvio-padrão e efetuada análise de variância e teste de Tuckey “a posteriori”. Resultados: A fagocitose (%) e a concentração de glicose (mmol/l) dos animais expostos por 1, 5 e 10 dias e do grupo controle foram respectivamente, 71,70±2,03 e 0,36±0,19; 70,0±4,73 e 0,88±0,47; 63,80±3,67 e 0,18±0,03 e 85,65±2,81 e 2,41±0,74. O grupo controle apresentou maior índice fagocitário (P>0,05) e os animais expostos durante 10 dias mostraram o índice inferior (P>0,05). Observou-se menor concentração de glicose em todos os grupos expostos em relação ao controle (P>0,05). Conclusão: Os resultados corroboram a hipótese de imunodepressão pela exposição à UVA

Porcentages phagocytosis’ evaluation uva irradiation effects of haemocytes and glucose’s concentration haemolymph in pink-shrimp Farfantepenaeus paulensis

Submitted by Francine Oliveira ([email protected]) on 2011-06-15T12:42:02Z No. of bitstreams: 1 Avaliação do efeito da radiação ultravioleta.pdf: 88932 bytes, checksum: 38271bb6551925b5896ecea67f098d08 (MD5)Approved for entry into archive by Barbara Milbrath([email protected]) on 2011-06-15T17:20:13Z (GMT) No. of bitstreams: 1 Avaliação do efeito da radiação ultravioleta.pdf: 88932 bytes, checksum: 38271bb6551925b5896ecea67f098d08 (MD5)Made available in DSpace on 2011-06-15T17:20:13Z (GMT). No. of bitstreams: 1 Avaliação do efeito da radiação ultravioleta.pdf: 88932 bytes, checksum: 38271bb6551925b5896ecea67f098d08 (MD5) Previous issue date: 2008Os crustáceos estão naturalmente expostos à radiação ultravioleta, podendo esta produzir mudanças bioquímicas e na função imune e predispor ao aparecimento de doenças. Objetivo: Quantificar a concentração de glicose na hemolinfa e avaliar o índice fagocitário em hemócitos de camarões Farfantepenaeus paulensis expostos à radiação UVA. Metodologia: 21 camarões foram divididos em três grupos submetidos à radiação UVA (tratamentos de 1, 5 e 10 dias) e grupo controle. A fagocitose foi efetuada em lâminas, nas quais os hemócitos foram avaliados pela sua capacidade de ingerir leveduras (Sacharomyces cerevisae). A glicose foi determinada por método enzimático (glicose oxidase). Os resultados foram expressos como média ± desvio-padrão e efetuada análise de variância e teste de Tuckey “a posteriori”. Resultados: A fagocitose (%) e a concentração de glicose (mmol/l) dos animais expostos por 1, 5 e 10 dias e do grupo controle foram respectivamente, 71,70±2,03 e 0,36±0,19; 70,0±4,73 e 0,88±0,47; 63,80±3,67 e 0,18±0,03 e 85,65±2,81 e 2,41±0,74. O grupo controle apresentou maior índice fagocitário (P>0,05) e os animais expostos durante 10 dias mostraram o índice inferior (P>0,05). Observou-se menor concentração de glicose em todos os grupos expostos em relação ao controle (P>0,05). Conclusão: Os resultados corroboram a hipótese de imunodepressão pela exposição à UVA.The crustacean are naturally exposed to ultraviolet radiation, it can produce biochemical and in immune function changes and predispose to diseases manifestation. Objective: To quantify the haemolymph glucose concentration and evaluate the phagocitary index of haemocytes in shrimp Farfantepenaeus paulensis exposed to UVA irradiation. Methodology: 21 shrimps were divided in one control group and 3 treatment groups exposed to UVA radiation for 1, 5 and 10 days. Phagocytosis was carried out in slide glass, the haemocytes capacity to engulf heat-killed yeast (Saccharomyces cerevisae) were analyzed. Glucose was determined by enzymatic method (oxidase glucose). The results were expressed by mean ± standard deviations and analyzed by ANOVA follow Tuckey test. Results: Phagocytosis (%) and the glucose concentration (mmol/l) of the animals exposed for 1, 5 and 10 days and control group were respectively, 71.70±2.03 and 0.36±0.19; 70.0±4.73 and 0.88±0.47; 63.80±3.67 and 0.18±0.03 and 85.65±2.81 and 2.41±0.74. Control group showed higher phagocytosis index (P>0.05) and the exposition during 10 days impaired the index (P>0.05). It was observed lower concentration of glucose in groups exposed than to control group (P>0.05). Conclusion: The results reached with the hypothesis of immunosuppression by the exposition to UVA

Avaliação do efeito da radiação ultravioleta “A” sobre porcentagens de fagocitose em hemócitos e concentração da glicose na hemolinfa do camarão-rosa Farfantepenaeus paulensis

Os crustáceos estão naturalmente expostos à radiação ultravioleta, podendo esta produzir mudanças bioquímicas e na função imune e predispor ao aparecimento de doenças. Objetivo: Quantificar a concentração de glicose na hemolinfa e avaliar o índice fagocitário em hemócitos de camarões Farfantepenaeus paulensis expostos à radiação UVA. Metodologia: 21 camarões foram divididos em três grupos submetidos à radiação UVA (tratamentos de 1, 5 e 10 dias) e grupo controle. A fagocitose foi efetuada em lâminas, nas quais os hemócitos foram avaliados pela sua capacidade de ingerir leveduras (Sacharomyces cerevisae). A glicose foi determinada por método enzimático (glicose oxidase). Os resultados foram expressos como média ± desvio-padrão e efetuada análise de variância e teste de Tuckey “a posteriori”. Resultados: A fagocitose (%) e a concentração de glicose (mmol/l) dos animais expostos por 1, 5 e 10 dias e do grupo controle foram respectivamente, 71,70±2,03 e 0,36±0,19; 70,0±4,73 e 0,88±0,47; 63,80±3,67 e 0,18±0,03 e 85,65±2,81 e 2,41±0,74. O grupo controle apresentou maior índice fagocitário (P>0,05) e os animais expostos durante 10 dias mostraram o índice inferior (P>0,05). Observou-se menor concentração de glicose em todos os grupos expostos em relação ao controle (P>0,05). Conclusão: Os resultados corroboram a hipótese de imunodepressão pela exposição à UVA.The crustacean are naturally exposed to ultraviolet radiation, it can produce biochemical and in immune function changes and predispose to diseases manifestation. Objective: To quantify the haemolymph glucose concentration and evaluate the phagocitary index of haemocytes in shrimp Farfantepenaeus paulensis exposed to UVA irradiation. Methodology: 21 shrimps were divided in one control group and 3 treatment groups exposed to UVA radiation for 1, 5 and 10 days. Phagocytosis was carried out in slide glass, the haemocytes capacity to engulf heat-killed yeast (Saccharomyces cerevisae) were analyzed. Glucose was determined by enzymatic method (oxidase glucose). The results were expressed by mean ± standard deviations and analyzed by ANOVA follow Tuckey test. Results: Phagocytosis (%) and the glucose concentration (mmol/l) of the animals exposed for 1, 5 and 10 days and control group were respectively, 71.70±2.03 and 0.36±0.19; 70.0±4.73 and 0.88±0.47; 63.80±3.67 and 0.18±0.03 and 85.65±2.81 and 2.41±0.74. Control group showed higher phagocytosis index (P>0.05) and the exposition during 10 days impaired the index (P>0.05). It was observed lower concentration of glucose in groups exposed than to control group (P>0.05). Conclusion: The results reached with the hypothesis of immunosuppression by the exposition to UVA

Time-course Expression of DNA Repair-related Genes in Hepatocytes of Zebrafish (Danio rerio) After UV-B Exposure

The objective of this study was to evaluate the time-course effects of UV-B exposure on expression of genes involved in the DNA repair system of zebrafish (Danio rerio)hepatocytes, a highly competent species in terms of damage repair induced by UV radiation. For gene expression analysis (RT-PCR), cells were exposed to 23.3 mJ cm)2 UV-B, which was the dose that affected viable cell number (reduction of 30% when compared with the control group) and produced no visual alteration on cell morphology. The early response observed(6h) showed induction in the expression of the CDKI gene (cyclin-dependent kinase inhibitor) and genes related to DNA damage repair (mainly XPC and DDB2), while the late response observed(24 h) was more related to up-regulation of p53 and genes involved in cell cycle arrest (gadd45a, cyclinG1). In all times analyzed, the antiapoptotic gene Bcl-2 was down-regulated. Another interesting result observed was the up-regulation of the Apex-1 gene after UV-B exposure, which could indicate the induction of oxidative lesions in the DNA molecule. In conclusion, these results demonstrate an activation of the DNA repair system in hepatocytes of zebrafish exposed to UV-B radiation, mainly involving the participation of p53

Relationship between multidrug resistance (MDR) and stem cell markers in human chronic myeloid leukemia cell lines

The K562 cell line (chronic myeloid leukemia), sensitive to chemotherapy (non-MDR), and the Lucena cell line, resistant to chemotherapy (MDR) were investigated. The results suggest that both cell lines possess CD34 + CD38 − profiles of hematopoietic stem cell markers. The promoter regions of ABCB1, ABCG2 and ABCC1 genes contain binding sites for the Oct-4 transcripton factor,which is also considered a marker of tumor stem cells. Lucena cells showed an over-expression of the ABCB1 gene and a high expression of the Oct-4, ABCG2 and ABCC1 genes as compared to K562 cells

Reactive oxygen species generation and expression of DNA repair-related genes after copper exposure in zebrafish (Danio rerio) ZFL cells

Copper is an essential metal to aquatic animals, but it can be toxic when in elevated concentrations in water. The objective of the present study was to analyze copper effects in zebrafish hepatocytes (ZFL cell-line). The number of viable cells and copper accumulation were determined in hepatocytes exposed in vitro to different copper concentrations (5–30mgCu/L). Intracellular reactive oxygen species (ROS) formation, total antioxidant capacity against peroxyl radicals, and expression of genes related do DNA repair system were also measured in hepatocytes exposed to 5 and 20mgCu/L. After 24 h of exposure,hepatocytes showed an exponential kinetics of copper accumulation. Copper exposure (24 and 48 h)significantly reduced hepatocytenumber in all concentrations tested, except at the lowest one (5mgCu/L). Exposure to 20mg Cu/L for 6, 12 and 24 h significantly increased intracellular ROS formation. However, no significant change intotal antioxidant capacitywas observed.After 12 and 24 hof exposure to20mg Cu/L, a significant decrease in expression of p53 and CDKI geneswas observed. Conversely,expression of Gadd45˛,CyclinG1 and Bax genes was significantly induced after 24 h of exposure to 20mg Cu/L.In hepatocytes exposed to 5mgCu/L, any significant alteration in expression of these genes was observed. In a broad view, most of genes encoding for DNA repair proteins were inhibited after copper exposure, especially in hepatocytes exposed to 20mg Cu/L. Taken all together, results obtained suggest that the increased intracellular ROS formation induced by copper exposure would be responsible for the alteration in gene expression pattern observed

Ultraviolet Radiation Induces Dose-Dependent Pigment Dispersion in Crustacean Chromatophores

Pigment dispersion in chromatophores as a response to UV radiation was investigated in two species of crustaceans, the crab Chasmagnathus granulata and the shrimp Palaemonetes argentinus. Eyestalkless crabs and shrimps maintained on either a black or a white background were irradiated with different UV bands. In eyestalkless crabs the significant minimal effective dose inducing pigment dispersion was 0.42 J/cm2 for UVA and 2.15 J/cm2 for UVB. Maximal response was achieved with 10.0 J/cm2 UVA and 8.6 J/cm2 UVB. UVA was more effective than UVB in inducing pigment dispersion. Soon after UV exposure, melanophores once again reached the initial stage of pigment aggregation after 45 min. Aggregated erythrophores of shrimps adapted to a white background showed significant pigment dispersion with 2.5 J/cm2 UVA and 0.29 J/cm2 UVC. Dispersed erythrophores of shrimps adapted to a black background did not show any significant response to UVA, UVB or UVC radiation. UVB did not induce any significant pigment dispersion in shrimps adapted to either a white or a black background. As opposed to the tanning response, which only protects against future UV exposure, the pigment dispersion response could be an important agent protecting against the harmful effects of UV radiation exposure

Methylene blue reverts multidrug resistance: sensitivity of multidrug resistant cells to this dye and its photodynamic action

Photodynamic action has been advocated as an alternative treatment of tumors but the most common used dyes, hematoporphyrin derivatives, are substrate for P-glycoprotein. This study investigated the MDR-reverting properties of methylene blue MB) and compared the sensitivity to its photodynamic action (PDA) in ®ve cell lines that either express or do not express the MDR phenotype. MB was able to revert the MDR phenotype and there was no difference in sensitivity to MB-PDA between MDR and non-MDR cells, suggesting that MB has the advantage of being used simultaneously as a MDR reverser and a photodynamic agent