Additional file 1: of C14orf132 gene is possibly related to extremely low birth weight

Timeline summarizing the clinical course of the children described in this case report. (PDF 210 kb

Additional file 1: of rs10732516 polymorphism at the IGF2/H19 locus associates with genotype-specific effects on placental DNA methylation and birth weight of newborns conceived by assisted reproductive technology

Table S1. Information of ART samples. (PDF 165 kb

Additional file 2: of rs10732516 polymorphism at the IGF2/H19 locus associates with genotype-specific effects on placental DNA methylation and birth weight of newborns conceived by assisted reproductive technology

Table S1. DNA methylation levels of H19 ICR (CTCF6), H19 DMR and LINE-1 in control and ART-derived placentas by EpiTYPER method. Methylation average values with SDs (±) of CpG units are presented. Gray boxes present significant methylation level difference at CpG sites in H19 DMR between ART and control samples within A/A genotype (nominal p-value < 0.05, Student’s t-test). Table S2. Total, rs10732516 genotype-specific (patG/matA and patA/matG) and allele-specific (paternal and maternal) DNA methylation levels at H19 ICR (CTCF6) in control and ART-derived placentas by traditional bisulfite sequencing. Gray boxes present significant methylation level difference at CpG sites between control and ART samples within genotypes and alleles (nominal p-value < 0.05, Mann-Whitney). Total methylation levels: total means of both genotypes and both alleles are included. Genotype-specific methylation levels: total means of both alleles are included. Allele-specific methylation levels: maternal and paternal alleles are presented separately. Table S3. rs10732516 patA/matG genotype- and allele-specific DNA methylation levels at H19 ICR1 (CTCF6) in controls and ART-derived newborns’ white blood cells (WBCs) in cord blood by traditional bisulfite sequencing. Genotype-specific methylation levels: total means of both alleles are included. Allele-specific methylation levels: maternal and paternal alleles are presented separately. (PDF 145 kb

Additional file 3: of rs10732516 polymorphism at the IGF2/H19 locus associates with genotype-specific effects on placental DNA methylation and birth weight of newborns conceived by assisted reproductive technology

Protocol S1. Extraction of total white blood cells (WBC) from EDTA-tube. Table S2. Primers (A) and PCR reactions (B). (PDF 306 kb