Lignani biljne vrste Achillea lingulata

Five lignans with a 2.6-diaryl-3.7-dioabicyclo[3,3.0]octane skeleton epieudesmin. kobusin, pinoresinol. fargesin and sesartemin, were isolated from the aerial parts and roots, of Achillea lingulata. Their structures were identified by comparison of their H-1-NMR and MS data to those in the literature. Fargesin and pinoresinol have not been isolated previously from any species of the genus Achillea.Iz nadzemnog dela i korena biljne vrste Achillea lingulata izolovano je pet lignana 2,6-diaril-3.7-dioksabicikloŠ3.3.0Ćoktanskog tipa. To su epieudesmin, kobusin, pinorezinol, fargezin i sezartemin. Izolovani lignani su identifikovani na osnovu identičnosti wihovih NMR i masenih spektara sa spektrima iz literature. Fargezin i pinorezinol nisu bili do sada izolovani iz biljnih vrsta roda Achillea

Supplementary material for the article: Petrović, Z. D.; Đorović, J.; Simijonović, D.; Trifunović, S.; Petrović, V. P. In Vitro Study of Iron Coordination Properties, Anti-Inflammatory Potential, and Cytotoxic Effects of N Salicylidene and N-Vanillidene Anil Schiff Bases. Chemical Papers 2018, 72 (9), 2171– 2180. https://doi.org/10.1007/s11696-018-0419-5

Supplementary material for: [https://doi.org/10.1007/s11696-018-0419-5]Related to published version: [http://cherry.chem.bg.ac.rs/handle/123456789/2190

Serum biomarkers of schizophrenia

Schizophrenia is a widespread mental disorder that leads to significant functional impairments and premature death. The state of the art indicates gaps in the understanding and diagnosis of this disease, but also the need for personalized and precise approaches to patients through customized medical treatment and reliable monitoring of treatment response. In order to fulfill existing gaps, the establishment of a universal set of disorder biomarkers is a necessary step. The investigation of serum samples of Serbian patients with schizophrenia (51) and healthy controls (39), based on NMR analyses based on detection and of low molecular weight components in samples can be difficult due to overlapping signal caused by line broadening characteristics of macromolecules. A way of overcoming this problem could be the exploitation of the difference in relaxation between small molecules and macromolecular species, such as the application of a T2 filter by using the Carr–Purcell–Meiboom–Gill (CPMG) spin-echo pulse sequence, which led to the identification of 26 metabolites/biomarkers for this disorder. The established biomarker set includes aspartate/aspartic acid, lysine, 2-hydroxybutyric acid, and acylglycerols, which are identified for the first time in schizophrenia serum samples by NMR experiments. The other 22 identified metabolites in the Serbian samples are in accordance with the previously established NMR-based serum biomarker sets of Brazilian and/or Chinese patient samples. Thirteen metabolites (lactate/lactic acid, threonine, leucine, isoleucine, valine, glutamine, asparagine, alanine, gamma-aminobutyric acid, choline, glucose, glycine and tyrosine) that are common for three different ethnic and geographic origins (Serbia, Brazil and China) could be a good start point for the setup of a universal NMR serum biomarker set for schizophrenia

Antifungalna aktivnost terpenoida davanonskog tipa izolovanih iz Artemisia lobelii Var. canescens

Five tetrahydrofuran sesquiterpenes, so-called davanones, and coumarin umbelliferone isolated from Artemisia lobelii All. var. canescens (DC.) Briqu, were tested for antifungal activity. All the compounds inhibited the growth of the applied fungi. The overall activity of one of them, 2-hydroxy-2,6,10-trimethyl-7,10-epoxy-dodeca-3,11-dien-5-on, was comparable to that of the antibiotic bifonazole.Pet tetrahidrofuranskih terpenoida davanonskog tipa i kumarin umbeliferon koji su prethodno izolovani iz biljne vrste Aretemisia lobelii var. conescens ispitivani su na antifungalnu aktivnost. Sva testirana jedinjenja inhibiraju rast primenjenih kultura gljiva. Najveću aktivnost, koja se može meriti sa aktivnošću komercijalnog antibiotika bifonazola, pokazuje 2-hidroksi-2,6,10-trimetil-7,10-epoksidodeka-3,11-dien-5-on

Composition, Antioxidant Potential, and Antimicrobial Activity of Helichrysum plicatum DC. Various Extracts

Helichrysum plicatum DC. is widely used in folk medicine in treating a variety of health disorders. The aim of this study was to examine the influence of different extraction solvents on the chemical composition, antioxidant potential, and antimicrobial activities of H. plicatum. Aerial parts were separately extracted with ethanol, dichloromethane, and sunflower oil. The oil extract (OE) was re-extracted with acetonitrile. A total of 142 compounds were tentatively identified in ethanolic (EE), dichloromethane (DCME), and acetonitrile (ACNE) extracts using HPLC-DAD/ESI-ToF-MS. The dominant compound class in all extracts were α-pyrones, alongside flavonoids in EE, terpenoids in DCME and ACNE, and phloroglucinols in DCME. The antioxidant potential of the extracts was assessed by the 2,2-diphenyl-1-picrylhydrazyl radical (DPPH) assay. EE and DCME possessed the most potent radical scavenging capacity. Antimicrobial activity was investigated on eight bacterial, two yeast, and one fungal species. All extracts exhibited high antifungal and notable antibacterial activities compared to control substances, with DCME being the most potent. DCME exhibited stronger antimicrobial activity against P. aeruginosa than the standard chloramphenicol

Antifungal activity of davanone-type sesquiterpenes from Artemisia lobelli var. conescens

Five tetrahydrofuran sesquiterpenes, so-called davanones, and coumarin umbelliferone isolated from Artemisia lobelii All. var. canescens (DC) Briqu, were tested for antifungal activity. All the compounds inhibited the growth of the applied fungi. The overall activity of one of them 2-hydroxy-2,6,10-trimethyl-7,10-epoxydodeca- 3,11-dien-5-on, was comparable to that of the antibiotic bifonazole.Pet tetrahidrofuranskih terpenoida davanonskog tipa i kumarin umbeliferon koji su prethodno izolovani iz biljne vrste Aretemisia lobelii var. conescens ispitivani su na antifungalnu aktivnost. Sva testirana jedinjenja inhibiraju rast primenjenih kultura gljiva. Najveću aktivnost, koja se može meriti sa aktivnošću komercijalnog antibiotika bifonazola, pokazuje 2-hidroksi-2,6,10-trimetil-7,10-epoksidodeka-3,11-dien-5-on.Projekat ministarstva br. 175

Synthesis and antioxidant activity of 1,3,4-oxadiazoles and their diacylhydrazine precursors derived from phenolic acids

Eight 1,3,4-oxadiazole derivatives containing phenolic acid moieties (7a-h) and eight of their diacylhydrazine precursors (6a-h) were synthesized, characterized using spectroscopic methods and examined by scavenging of stable DPPH (2,2-diphenyl-1-picrylhydrazyl) radicals. The most potent phenolic 1,3,4-oxadiazoles showed better DPPH scavenging activity in comparison with their corresponding diacylhydrazine precursors as a result of participation of both aromatic rings and a 1,3,4-oxadiazole moiety in resonance stabilization of the formed phenoxyl radical. Four diacylhydrazines (6d, 6e, 6g, and 6h) and four 1,3,4-oxadiazoles (7d, 7e, 7g and 7h) with the best DPPH scavenging activity, were chosen for further evaluation of their antioxidant potential through various assays. The investigated compounds exerted pronounced ABTS radical scavenging capacity, moderate to good H2O2 scavenging properties and strong ferric ion reducing capacity. Further in vitro evaluation of the antioxidant properties of the most active compounds demonstrated their protective effects in normal lung fibroblasts MRC-5 against hydrogen peroxide induced oxidative stress. Diacylhydrazine 6h increased two times the activity of glutathione peroxidase in treated cells in comparison with a control sample and did not affect the superoxide dismutase activity

Regioselektivnost konjugovane adicijena monoalkil-1,4-benzohinone

The regioselectivity of the reaction of conjugate addition of thiols, amines, methanol and hydrogen chloride with the monoalkyl-1,4-benzoquinones avarone and 2-tert-butyl-1,4-benzoquinone was investigated. It was shown that the regioselectivity of the reaction is influenced by the electrophilicity of position 5 in unprotonated 2-alkylquinones, the increased electrophilicity of position 6 in acidic medium, and by the acidity of the intermediate hydroquinones.Proučavana je regioselektivnost konjugovane adicije tiola, amina, metanola i hlorovodonika na monoalkil-1,4-benzohinone avaron i 2-tert-butil-1,4-benzohinon. Pokazano je da na regioselektivnost reakcije utiču elektrofilnost položaja 5 neprotonovanih 2-alkil-hinona i povećana elektrofilnost položaja 6 u kiseloj sredini, kao i kiselost intermedijernih hidrohinona

Volatile Constituents of Different Plant Parts and Populations of Malabaila aurea Boiss. from Montenegro

The volatile constituents of different plant parts and populations of Malabaila aurea Boiss. from Montenegro were obtained by simultaneous distillation-extraction and analyzed by GC-FID and GC-MS. A total of 12 samples were examined and 45 compounds were identified. The volatile content of different M. aurea populations was very similar, while the volatile fractions obtained from different plant parts showed significant qualitative and quantitative differences. The most abundant compounds found in stems & leaves were apiole (51.0-56.3%), myristicin (16.3-25.4%), and falcarinol (4.1-10.7%). The roots showed the same major components, but with different relative abundances: 30.9-49.1% of apiole, 12.9-34.7% of falcarinol, and 9.9-31.1% of myristicin. The volatile constituents of fruits & flowers were remarkably different, containing up to 71.2-80.5% octyl butyrate, 11.4-18.0% octanol, and 2.7-6.8% octyl hexanoate. The results were discussed as possible indication of relatedness of Malabaila aurea and Pastinaca sativa (parsnip)

Hemijske karakteristike visoko-krečnjačkih zemljišta koje određuju raspodelu fosfora

Phosphorus fractions from three highly calcareous soils (average, 24.9 +/- 4.8 %CO32-) from sampling sites with a Mediterranean climate were isolated by sequential extraction. In order to provide a more reliable basis for the definition of the obtained P-fractions, principal component analysis was applied and from the chemical characteristics of the 14 investigated soils, those characteristics which define at he content and association features of the P-fractions were assessed. The soils are characterized by a relatively high pH (8.0-8.2) and by significantly differing contents of organic mater, acid-soluble Mg and total P. These differences affected the various association features of the P-fraction with the soil constituents. The NH4F-P fraction (isolated with 0.5 M NH4F, pH 8.2) is defined by the contents of the main metals of the oxide-hydroxide-clay associations (Al, Fe, Mn) or by the the redox potential (Eh) of Mn. The accumulation of NaOH-phosphorus (extractable with 0.1 M NaOH) depended on the constituents of the oxide hydroxide-clay association, the humic substances and Eh-related factors. In those soils in which NaOH-P is defined by the oxide-hydroxide-clay association, the participation of Fe as a bridge-forming metal is proposed. The main part of total P, i.e., Delta P = TP - (NH4F-P + NaOH-P) is defined by the status of Mn- and Fe-humic complexes or by the concentration of hydroxyl-ions.Iz tri visoko-krečnjačka zemljišta (prosečni sadržaj CO 3 2- 24.9 ± 4.8%, pH 8.0 - 8.2), sa lokacija sa sredozemnom klimom, izolovane su frakcije fosfora sekvencijalnom ekstrakcijom. U cilju obezbeđivanja pouzdanije osnove za definisanje dobijenih frakcijafosfora (P-frakcija), primenjena je analiza principalne komponente (PC-analiza). Među 14 hemijskih karakteristika zemljišta, izdvojene su one koje definišu sadržaj pojedinih P-frakcija i prirodu njihove asocijacije sa sastojcima zemljišta. Frakcija NH 4-P (izolovana sa 0,5M NH 4F, pH = 8,2) definisana je sadržajem glavnih metala oksidne-hidroksidne-glinene asocijacije (Al, Fe, Mn) ili Eh-statusom izraženim sadržajem Mn. Akumulacija NaOH-P (ekstrahovanog sa 0,1 M NaOH) zavisila je od sastojaka oksidne-hidroksidne-glinene asocijacije, od huminskih supstanci i od faktora koji stoje u vezi sa Eh. U onim zemljištima u kojima je NaOH-P definisana sastojcima oksidne-hidroksidne-glinene asocijacije, pretpostavljeno je vezivanje fosfora preko Fe-mostova. Glavna količina fosfora ∆P = TP - (NH 4F-P + NaOH-P), u pojedinim zemljištima definisana je ili statusom Mn- i Fe-huminskih kompleksa, ili pH-vrednošću