3 research outputs found
In vitro effects of regulators on growth and morphogenesis of Ocimum basilicum L. ‘Alfavaca Green’ stem apexes
Large-scale cultivation of contamination free plants requires a good standardization
protocol and production methods. Basil is widely used for cosmetics, food and pharmaceutical
industries as it is rich in many bioactive compounds. This present study aimed to evaluate the
growth and in vitro anatomical aspects of apical buds of basil grown under
different concentrations rowth regulators like: NAA (Naphthalenoacetic Acid), BAP
(6-benzylaminopurine), and KIN (Kinetin). The in vitro establishment was evaluated every 20
days to calculate the, the percentage of plants with calluses, appearance of the roots, any abnormal
seedlings, any oxidized seedlings, and the number of sprouts per plant. Growth, physiological,
and morpho-anatomical evaluations were performed at 80 days. Basal callogenesis was observed
when cytokinin’s and auxins are used in combination. Auxin treatments caused hyperhydricity in
the stems and leaves. Medium A2 (0.05 mg L
-1 of NAA and 0.1 mg L
-1 of BAP), and A3
(0.05 mg L
-1 of NAA and 0.1 mg L
-1 of KIN) resulted in the best development of basil plants,
cultivar ‘Alfavaca Green’. The A2 produced plants with greater numbers of leaves, an average
bud length of 59.81 mm, and the best root properties. A2 and A1 have a higher percentage of
hyperhydricity (83 and 67%). The A3 resulted in an acceptable number of leaves (range: 21–39),
and this treatment produced the best shoot properties as well as fewer plants with hyperhydricity.
In addition, the A3 treatment produced plants with a shoot length, high shoot fresh and dry mass
(2.82 and 0.23 g), high chlorophyll index and leaf anatomy that was similar to the control.
Excluding the control, the other treatments presented more than 90% of the explants with calluses
in their bases
Copper modulates the biochemical and enzymatic activity and growth of tomato cultivars grown in vitro
Received: August 25th, 2020 ; Accepted: December 17th, 2020 ; Published: February 09th, 2021 ; Correspondence: [email protected] (Cu) is a micronutrient that is neglected for tomato growth. This study sought
to identify the effects of exposure to Cu on the growth and biochemical activity of two tomato
cultivars. Tomato seeds of ‘Carolina’ and ‘Cereja’ cultivars were disinfected and inoculated in
MS medium plus copper sulfate concentrations (CuSO4) (default MS, 25, 50, and 100 µm) and
had their growth monitored for 30 days. It was estimated that the growth and biomass
accumulation of tomato plants ‘Carolina’ and ‘Cereja’, both from the aerial part and the roots,
were benefited by 25 e 50 µm of CuSO4. However, it was observed that these concentrations were
inefficient in controlling hyperhydricity and leaf deformation. There was a reduction of these
phenomena in the treatment with 100 µm, in both cultivars. Tomato of ‘Carolina’ cultivar
subjected to 100 µm showed an increase in anthocyanins and superoxide dismutase (SOD)
activity in the root system. There was a reduction of catalase (CAT) activity in shoots exposed to
Cu. ‘Cereja’ tomatoes subjected to 100 µm showed an increase in CAT and SOD activity in
shoots and roots, respectively. It was concluded that the ‘Carolina’ and ‘Cereja’ tomatoes have
their growth impaired when exposed to 100 µm CuSO4. Concentrations higher than 50 µm of
CuSO4 cause an increase in the antioxidant activity in the shoot of tomato plants from the
‘Carolina’ cultivar. Concentrations higher than 50 µm CuSO4 increase SOD activity in the root
system of tomato plants from the ‘Cereja’ cultivar