23 research outputs found
Remodeling of Fibrous Extracellular Matrices by Contractile Cells: Predictions from Discrete Fiber Network Simulations
Contractile forces exerted on the surrounding extracellular matrix (ECM) lead
to the alignment and stretching of constituent fibers within the vicinity of
cells. As a consequence, the matrix reorganizes to form thick bundles of
aligned fibers that enable force transmission over distances larger than the
size of the cells. Contractile force-mediated remodeling of ECM fibers has
bearing on a number of physiologic and pathophysiologic phenomena. In this
work, we present a computational model to capture cell-mediated remodeling
within fibrous matrices using finite element based discrete fiber network
simulations. The model is shown to accurately capture collagen alignment,
heterogeneous deformations, and long-range force transmission observed
experimentally. The zone of mechanical influence surrounding a single
contractile cell and the interaction between two cells are predicted from the
strain-induced alignment of fibers. Through parametric studies, the effect of
cell contractility and cell shape anisotropy on matrix remodeling and force
transmission are quantified and summarized in a phase diagram. For highly
contractile and elongated cells, we find a sensing distance that is ten times
the cell size, in agreement with experimental observations.Comment: Accepted for publication in the Biophysical Journa
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Micropatterned Multicolor Dynamically Adhesive Substrates to Control Cell Adhesion and Multicellular Organization
We present a novel technique to examine cell–cell interactions and directed cell migration using micropatterned substrates of three distinct regions: an adhesive region, a nonadhesive region, and a dynamically adhesive region switched by addition of a soluble factor to the medium. Combining microcontact printing with avidin–biotin capture chemistry, we pattern nonadhesive regions of avidin that become adhesive through the capture of biotinylated fibronectin. Our strategy overcomes several limitations of current two-color dynamically adhesive substrates by incorporating a third, permanently nonadhesive region. Having three spatially and functionally distinct regions allows for the realization of more complex configurations of cellular cocultures as well as intricate interface geometries between two cell populations for diverse heterotypic cell–cell interaction studies. We can now achieve spatial control over the path and direction of migration in addition to temporal control of the onset of migration, enabling studies that better recapitulate coordinated multicellular migration and organization in vitro. We confirm that cellular behavior is unaltered on captured biotinylated fibronectin as compared to printed fibronectin by examining the cells’ ability to spread, form adhesions, and migrate. We demonstrate the versatility of this approach in studies of migration and cellular cocultures, and further highlight its utility by probing Notch–Delta juxtacrine signaling at a patterned interface
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A DNA-based molecular probe for optically reporting cellular traction forces
We developed molecular tension probes (TPs) that report traction forces of adherent cells with high spatial resolution, can be linked to virtually any surface, and obviate monitoring deformations of elastic substrates. TPs consist of DNA hairpins conjugated to fluorophore-quencher pairs that unfold and fluoresce when subjected to specific forces. We applied TPs to reveal that cellular traction forces are heterogeneous within focal adhesions and localized at their distal edges
3D biomimetic environment enabling ex utero trophoblast invasion and co-culture of embryos and somatic cells
Summary: The first direct contact between the embryo and the mother is established during implantation. This process is inaccessible for direct studies as the implanting embryo is concealed by the maternal tissues. Here, we present a protocol for establishing a 3D biomimetic environment based on synthetic hydrogels which harbor key biomechanical properties of the uterine stroma. We describe steps for isolating and culturing embryos in PEG/DexMA hydrogel. We then detail the co-culture of embryos and endothelial cells in a microfluidic device.For complete details on the use and execution of this protocol, please refer to Govindasamy et al. (2021)1 and Ozguldez et al. (2023).2 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics
Interaction between single-walled carbon nanotubes and alkyl-polyglycerol derivatives
We use three alkyl-polyglycerol derivatives to suspend singlewalled
carbon nanotubes. The molecules differ by the aromatic
moieties between the alkyl tail and the polyglycerol head. The
suspended nanotubes are analysed by photoluminescence
spectroscopy. We observe a dependence of the luminescence
intensity and hence nanotube abundance on the aromatic
moieties. Interestingly, the strength of interaction depends on
the nanotube families
Nonswelling and Hydrolytically Stable Hydrogels Uncover Cellular Mechanosensing in 3D
While matrix stiffness regulates cell behavior on 2D substrates, recent studies using synthetic hydrogels have suggested that in 3D environments, cell behavior is primarily impacted by matrix degradability, independent of stiffness. However, these studies did not consider the potential impact of other confounding matrix parameters that typically covary with changes in stiffness, particularly,�hydrogel swelling and hydrolytic stability, which may explain the previously observed distinctions in cell response in 2D versus 3D settings. To investigate how cells sense matrix stiffness in 3D environments, a nonswelling, hydrolytically stable, linearly elastic synthetic hydrogel model is developed in which matrix stiffness and degradability can be tuned independently. It is found that matrix degradability regulates cell spreading kinetics, while matrix stiffness dictates the final spread area once cells achieve equilibrium spreading. Importantly, the differentiation of human mesenchymal stromal cells toward adipocytes or osteoblasts is regulated by the spread state of progenitor cells upon initiating differentiation. These studies uncover matrix stiffness as a major regulator of cell function not just in 2D, but also in 3D environments, and identify matrix degradability as a critical microenvironmental feature in 3D that in conjunction with matrix stiffness dictates cell spreading, cytoskeletal state, and stem cell differentiation outcomes.This study establishes a linearly elastic, 3D hydrogel cell culture model, in which matrix stiffness, adhesiveness, and degradability can be independently tuned without concurrent changes in other hydrogel properties, in particular swelling and hydrolytic stability. Using this model, the authors determine that matrix degradability regulates cell spreading kinetics, while matrix stiffness dictates the final spread state once cells achieve equilibrium spreading.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/172353/1/advs3653-sup-0001-SuppMat.pdfhttp://deepblue.lib.umich.edu/bitstream/2027.42/172353/2/advs3653_am.pdfhttp://deepblue.lib.umich.edu/bitstream/2027.42/172353/3/advs3653.pd
Amphiphile replacement on carbon nanotube surfaces: Effect of aromatic groups on the interaction strength
Carbon nanotubes (CNTs) were solubilized using akyl/polyglycerol amphiphiles. Similar cosurfactants, bearing different aromatic moieties between head and tail, were added to these samples. The interaction strength between these amphiphiles and CNTs changes depending on the inserted aromatic moieties. The insertion of a phenyl ring allows the amphiphile to replace the starting one indicating a higher interaction strength, while the insertion of a triazol pentagon does not, suggesting that the interaction strength is lower. The replacement was monitored via PLE mapping
Mechanically induced generation of counterions inside surface-grafted charged macromolecular films: Towards enhanced mechanotransduction in artificial systems
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Matrix degradability controls multicellularity of 3D cell migration
A major challenge in tissue engineering is the development of materials that can support angiogenesis, wherein endothelial cells from existing vasculature invade the surrounding matrix to form new vascular structures. To identify material properties that impact angiogenesis, here we have developed an in vitro model whereby molded tubular channels inside a synthetic hydrogel are seeded with endothelial cells and subjected to chemokine gradients within a microfluidic device. To accomplish precision molding of hydrogels and successful integration with microfluidics, we developed a class of hydrogels that could be macromolded and micromolded with high shape and size fidelity by eliminating swelling after polymerization. Using this material, we demonstrate that matrix degradability switches three-dimensional endothelial cell invasion between two distinct modes: single-cell migration and the multicellular, strand-like invasion required for angiogenesis. The ability to incorporate these tunable hydrogels into geometrically constrained settings will enable a wide range of previously inaccessible biomedical applications
Polyglycerol-Derived Amphiphiles for the Solubilization of Single-Walled Carbon Nanotubes in Water: A Structure-Property Study
A series of nonionic amphiphiles derived from polyglycerol dendrons were studied for their ability to solubilize and isolate single-walled carbon nanotubes. The amphiphiles possessed differently sized polar head groups, hydrophobic tail units, and various aromatic and non-aromatic groups between the head and tail groups. Absorbance analysis revealed that amphiphiles with anchor groups derived from pyrene were far inferior to those that possessed simple linear aliphatic tail groups. Absorbance and near-infrared fluorescence analyses revealed a weak dependence on the dendron size of the head group, but a strong positive trend in suspended nanotube density and fluorescence intensity for amphiphiles with longer tail units. Variations in the moieties linking the head and tail groups led to a range of effects on the suspensions, with linkers imparting flexibility and a bent shape that gave improved performance overall. This was illustrated most dramatically by a pair of benzamide-containing amphiphiles, the para isomer of which showed evidence in the fluorescence data of increased nanotube aggregate formation when compared with the meta isomer. In addition, statistical AFM was used to illustrate more directly the microscopic differences between amphiphiles that were effective at nanotube bundle disruption and those that were not