Common genetic variation in a basal promoter element alters DDAH2 expression in endothelial cells

Synthesis of the vasodilator nitric oxide (NO) can be inhibited by the endogenous methylarginines l-NMMA and ADMA. ADMA is elevated in a number of cardiovascular disorders in which NO availability is reduced. Elimination of ADMA from the body occurs primarily by enzymatic breakdown through the action of DDAH, of which two isoforms exist, DDAH1 and DDAH2. In this study we have identified a core promoter region of the DDAH2 gene, and transcription factor sites that play an important role in the regulation of DDAH2 expression. Using PCR-SSCP analysis we also identified six common polymorphisms. One of these polymorphisms (an insertion/deletion at position –871) within the core promoter element influenced basal transcription. The discovery of a functional polymorphism within the DDAH2 promoter suggests that there may be common, individual differences in the ability to metabolise ADMA in vivo, that in turn, might underlie susceptibility to cardiovascular disease

Impacts of Payment for Forest Environmental Services in Cat Tien National Park

This paper assesses the impacts of Payment for Forest Environmental Services (PFES) in Cat Tien National Park, Vietnam. We analyze the impacts and additionality of PFES on local livelihoods by comparing the socio-economic situations in four pairs of villages before and after its implementation, and between places where PFES is and is not applied. In total, 149 people participated in focus group discussions, while 244 households (123 in areas with PFES and 121 in areas without) took part in household surveys. Our research shows that 92% of the people interviewed are from ethnic minorities participating and benefiting directly from PFES. In villages with PFES, the numbers of participating households ranged from 45% to 88% of all poor households in those villages. Of the poor households participating in PFES in the studied villages, 22% have no source of cash income other than their forest protection contracts, while 81.4% have escaped poverty, based on self-defined poverty criteria, through additional income from forest protection. Since the implementation of PFES, the area of forests allocated for community and household management is estimated to be three to 3.64 times higher than it had been previously. Although the number of communities under PFES contracts has not changed, the number of households participating in forest protection contracts is now much lower than before PFES started. On average, PFES contributes 16% to 74% of total household income in villages with PFES. Incomes in places with PFES are significantly higher than in places without. Although our research demonstrates immediate positive socio-economic impacts on livelihoods, it also highlights weaknesses in the current monitoring and evaluation system and a lack of reliable data for measuring PFES impacts in Cat Tien National Park