Mutagenesis of a cAMP Response Element within the Latency-Associated Transcript Promoter of HSV-1 Reduces Adrenergic Reactivation

AbstractMutagenesis of a cyclic AMP response element (CRE) within the LAT promoter of HSV-1 reduces the ability of LAT expression to be induced in transient assays, but has only a minimal impact on reactivation of the virus inin vitrosystems. Here we show that a CRE mutation results in a significant reduction of adrenergically induced reactivationin vivoin the rabbit eye model. Spontaneous reactivation frequencies were also reduced. In addition, we demonstrate that this mutation has no effect on the amount of LAT expressed during latency when compared with the parent, 17syn+, and the rescuant. These results indicate a greater effect of CRE on induced reactivationin vivothan inin vitrosystems, but also suggest that the CRE in the LAT promoter is not autonomous in conducting the reactivation signal

Characterization of ENU-induced mutations in red blood cell structural proteins

Murine models with modified gene function as a result of N-ethyl-N-nitrosourea (ENU) mutagenesis have been used to study phenotypes resulting from genetic change. This study investigated genetic factors associated with red blood cell (RBC) physiology an

Using Digital Nudges on Analytics Dashboards to Reduce Anchoring Bias

This study investigates the effectiveness of digital nudging on reducing the anchoring bias observed in the use of supply chain management (SCM) software. A between-subjects experiment with 61 participants was conducted comparing a control group with two types of digital nudges implemented on an SCM analytics dashboard. Findings show that digital nudging can help mitigate an anchoring bias in several use conditions. Theoretical and practical contributions are discussed, which include that in addition to individual-level outcomes, digital nudging can also be applied in business environments to improve organizational-level performance

Gene transfer into the central nervous system using Herpes Simplex Virus-1 vectors

Manipulation of gene expression in developing or in mature central nervous systems (CNS) holds a promise for the resolution of many compelling neurobiological questions, including the feasibility of gene therapy to treat diseases of the brain. In this context, a number of viral vectors has been used in recent years to introduce and express genes into the CNS. This article discusses a gene transfer system based on the Herpes Simplex Virus-1 (HSV-1). We describe here the use of non-replicating, non-toxic HSV-1 vector, 8117/43, in a series of studies carried in our joint program. This vector proves further the utility of HSV-1 as a delivery vehicle to a number of distinct sites within the CNS

CW Interference Effects on High Data Rate Transmission Through the ACTS Wideband Channel

Satellite communications channels are susceptible to various sources of interference. Wideband channels have a proportionally greater probability of receiving interference than narrowband channels. NASA's Advanced Communications Technology Satellite (ACTS) includes a 900 MHz bandwidth hardlimiting transponder which has provided an opportunity for the study of interference effects of wideband channels. A series of interference tests using two independent ACTS ground terminals measured the effects of continuous-wave (CW) uplink interference on the bit-error rate of a 220 Mbps digitally modulated carrier. These results indicate the susceptibility of high data rate transmissions to CW interference and are compared to results obtained with a laboratory hardware-based system simulation and a computer simulation

Polarized Narrow-Line Emission from the Nucleus of NGC 4258

The detection of polarized continuum and line emission from the nucleus of NGC 4258 by Wilkes et al. (1995) provides an intriguing application of the unified model of Seyfert nuclei to a galaxy in which there is known to be an edge-on, rotating disk of molecular gas surrounding the nucleus. Unlike most Seyfert nuclei, however, NGC 4258 has strongly polarized narrow emission lines. To further investigate the origin of the polarized emission, we have obtained spectropolarimetric observations of the NGC 4258 nucleus at the Keck-II telescope. The narrow-line polarizations range from 1.0% for [S II] 6716 to 13.9% for the [O II] 7319,7331 blend, and the position angle of polarization is oriented nearly parallel to the projected plane of the masing disk. A correlation between critical density and degree of polarization is detected for the forbidden lines, indicating that the polarized emission arises from relatively dense (n_e > 10^4 cm^-3) gas. An archival Hubble Space Telescope narrow-band [O III] image shows that the narrow-line region has a compact, nearly unresolved core, implying a FWHM size of <2.5 pc. We discuss the possibility that the polarized emission might arise from the accretion disk itself and become polarized by scattering within the disk atmosphere. A more likely scenario is an obscuring torus or strongly warped disk surrounding the inner portion of a narrow-line region which is strongly stratified in density. The compact size of the narrow-line region implies that the obscuring structure must be smaller than ~2.5 pc in diameter.Comment: To appear in the Astronomical Journal. 13 pages, including 1 table and 4 figures. Uses emulateapj.st