NGHIÊN CỨU NHÂN GIỐNG IN VITRO VÀ KHẢO SÁT HỢP CHẤT ALKALOID ROTUNDINE TỪ CÂY BÌNH VÔI (STEPHANIA ROTUNDA LOUR

RESEARCH THE MICROPROPAGATION IN VITRO AND DETECTION ROTUNDINE OF STEPHANIA ROTUNDA LOUR. Binh voi (Vietnamese name), Stephannia Rotunda Lour is a wild herbal species often found in jungles of Binh Thuan, Ninh Thuan, Lam Dong and Dong Nai. This is a valuable medicinal herb that can treat some diseases, such as: enteritis, stomachache, especially depression. Recently, because of the exploitation and destruction of jungles, the Binh Voi will be extinct in near future is undeniable. Therefore, using preservation and restoration techniques is necessary and in vitro is a efficient one. Media have 3 - 10 mg/l of 2,4-D formed callus from cuttings of the Stephania rotunda Lour. The medium having higher concentration of minerals had a higher ability of forming callus. Having 1 mg/l BA and 0,2 mg/l of NAA in the medium stimulated the forming of shoots from buds on cuttings. The substance alkaloid rotundine, which can cure the depression, appeared on the stem, bulb (natural) and callus (in vitro) samples. And the highest concentration of alkaloid rotundine is from bulb samples

Antibacterial, antioxidant and cytotoxic activities of different fractions of acetone extract from flowers of Dipterocarpus intricatus Dyer (Dipterocarpaceae)

This study has shown for the first time the antimicrobial, antioxidant and cytotoxicity of 3 fractions of acetone extract, including hexane, chloroform and ethyl acetate from flowers of Dipterocarpus intricatus. Antibacterial test using disc diffusion method showed that the chloroform and ethyl acetate fractions inhibited the growth of all the tested bacteria, including Escherichia coli, Pseudomonas aeruginosa, Salmonella enteritidis, Salmonella typhimurium, Bacillus cereus and Staphylococcus aureus while the hexane fraction showed the antibacterial activity against B. cereus and S. enteritidis. Antioxidant activity and cancer cell resistance of those extracts were conducted using DPPH and MTT methods respectively. As a result, the DPPH radical scavenging activity of the hexane, chloroform and ethyl acetate fractions were determined with the IC50 values of 0.508, 0.22 and 0.075 mg/mL respectively while the cytotoxicity to HepG2 cell line of those fractions was 163.3 ppm, 106.7 ppm and 459.3 ppm. These results suggested the potential application of these fractions isolated from D. intricatus flowers as the natural antimicrobial, antioxidant and cytotoxic agents for medicine

Nghiên cứu nhân giống in vitro cây Chè Mã Dọ (Camellia sp.) : Tóm tắt Luận văn Thạc sĩ Ngành Sinh học thực nghiệm

25 tr. : minh hoạ (1 phần màu) ; 21 cm

Nghiên cứu nhân giống in vitro cây Chè Mã Dọ (Camellia sp.) : Luận văn Thạc sĩ Ngành Sinh học thực nghiệm

xi, 82 tr. : minh hoạ (1 phần màu) ; 29 cm

Đại số tuyến tính

318 tr. ; 21c

Survey of VietGAP vegetables natural enemies and insect pests in Hoc Mon district and study on capacity of parasitism of endoparasitoid Cotesia plutellae Kurdjumov

Studies were conducted at four VietGAP standardvegetable fields (Hoc Mon District, Ho Chi Minh City) from August 2013 to April 2014 in order to investigate the species of insect pest, natural enemies on vegetablesgrownby VietGAP program. The results indicatedthat 60species, 43families, 23orders including 34insect pests, 26species of natural enemieswere found in this study. Moreover, parasitoid Cotesia plutellae had significantdifference in high rate of parasitism on second instar of Plutella xylostella in comparison with other instars. Natural enemiesknown asan important role in the regulation of the number of insect pestsare discussed