Biodesulfurization of dibenzothiophene by Shewanella putrefaciens NCIMB 8768

The desulfurization ability of Shewanella putrefaciens strain NCIMB 8768 was studied and its activity profile was compared with the widely studied strain Rhodococcus erythropolis strain IGTS8. Dibenzothiophene (DBT) is a recalcitrant thiophenic component of fossil fuels especially among diesel blend stocks. DBT in basic salt medium (BSM) at a final concentration of 0.3, 0.6 and 0.9 mM was supplied to the microbes as the sole sulfur source. Experimental results showed that S. putrefaciens, similar to other biodesulfurization organisms, converted DBT to the end product 2-hydroxybiphenyl (HBP), as detected by the Gibbs assay and HPLC. Cells cultivated in medium containing 0.3 mM of DBT showed the highest desulfurization activity, with a maximum specific production rate 43.5 mmol/L of HBP

Fabrication and optimisation of a fused filament 3D-printed microfluidic platform

A 3D-printed microfluidic device was designed and manufactured using a low cost ($2000) consumer grade fusion deposition modelling (FDM) 3D printer. FDM printers are not typically used, or are capable, of producing the fine detailed structures required for microfluidic fabrication. However, in this work, the optical transparency of the device was improved through manufacture optimisation to such a point that optical colorimetric assays can be performed in a 50 µl device. A colorimetric enzymatic cascade assay was optimised using glucose oxidase and horseradish peroxidase for the oxidative coupling of aminoantipyrine and chromotropic acid to produce a blue quinoneimine dye with a broad absorbance peaking at 590 nm for the quantification of glucose in solution. For comparison the assay was run in standard 96 well plates with a commercial plate reader. The results show the accurate and reproducible quantification of 0–10 mM glucose solution using a 3D-printed microfluidic optical device with performance comparable to that of a plate reader assay

DBT degradation enhancement by decorating Rhodococcus erythropolis IGST8 with magnetic Fe3O4 nanoparticles

Biodesulfurization (BDS) of dibenzothiophene (DBT) was carried out by Rhodococcus erythropolis IGST8 decorated with magnetic Fe3O4 nanoparticles, synthesized in-house by a chemical method, with an average size of 45-50 nm, in order to facilitate the post-reaction separation of the bacteria from the reaction mixture. Scanning electron microscopy (SEM) showed that the magnetic nanoparticles substantially coated the surfaces of the bacteria. It was found that the decorated cells had a 56% higher DBT desulfurization activity in basic salt medium (BSM) compared to the nondecorated cells. We propose that this is due to permeabilization of the bacterial membrane, facilitating the entry and exit of reactant and product, respectively. Model experiments with black lipid membranes (BLM) demonstrated that the nanoparticles indeed enhance membrane permeability

The ATLAS-SPT Radio Survey of Cluster Galaxies

Using a high-performance computing cluster to mosaic 4,787 pointings, we have imaged the 100 sq. deg. South Pole Telescope (SPT) deep-field at 2.1 GHz using the Australian Telescope Compact Array to an rms of 80 $\mu$Jy and a resolution of 8". Our goal is to generate an independent sample of radio-selected galaxy clusters to study how the radio properties compare with cluster properties at other wavelengths, over a wide range of redshifts in order to construct a timeline of their evolution out to $z \sim 1.3$. A preliminary analysis of the source catalogue suggests there is no spatial correlation between the clusters identified in the SPT-SZ catalogue and our wide-angle tail galaxies.Comment: 9 pages, 4 figures. Submitted to Proceedings of Science for "The many facets of extragalactic radio surveys: towards new scientific challenges", Bologna, Italy 20-23 October 2015 (EXTRA-RADSUR2015

The Spatial Correlation of Bent-Tail Galaxies and Galaxy Clusters

We have completed a deep radio continuum survey covering 86 square degrees of the Spitzer-South Pole Telescope deep field to test whether bent-tail galaxies are associated with galaxy clusters. We present a new catalogue of 22 bent-tail galaxies and a further 24 candidate bent-tail galaxies. Surprisingly, of the 8 bent-tail galaxies with photometric redshifts, only two are associated with known clusters. While the absence of bent-tail sources in known clusters may be explained by effects such as sensitivity, the absence of known clusters associated with most bent-tail galaxies casts doubt upon current models of bent-tail galaxies.Comment: Accepted by MNRA

ISO observations of M8, the Lagoon nebula

In this paper, IRAS, ISO, and molecular line observations of the M8 and M8E sources in the Lagoon Nebula are reported

Large-Scale CO Maps of the Lupus Molecular Cloud Complex

Fully sampled degree-scale maps of the 13CO 2-1 and CO 4-3 transitions toward three members of the Lupus Molecular Cloud Complex - Lupus I, III, and IV - trace the column density and temperature of the molecular gas. Comparison with IR extinction maps from the c2d project requires most of the gas to have a temperature of 8-10 K. Estimates of the cloud mass from 13CO emission are roughly consistent with most previous estimates, while the line widths are higher, around 2 km/s. CO 4-3 emission is found throughout Lupus I, indicating widespread dense gas, and toward Lupus III and IV. Enhanced line widths at the NW end and along the edge of the B228 ridge in Lupus I, and a coherent velocity gradient across the ridge, are consistent with interaction between the molecular cloud and an expanding HI shell from the Upper-Scorpius subgroup of the Sco-Cen OB Association. Lupus III is dominated by the effects of two HAe/Be stars, and shows no sign of external influence. Slightly warmer gas around the core of Lupus IV and a low line width suggest heating by the Upper-Centaurus-Lupus subgroup of Sco-Cen, without the effects of an HI shell.Comment: 54 pages, 27 figures, 5 tables. To appear in ApJS. Preprint also available (with full-size figures) from http://www.astro.ex.ac.uk/people/nfht/publications.html Datacubes available from http://www.astro.ex.ac.uk/people/nfht/resources.htm

Infrared-Faint Radio Sources: A New Population of High-redshift Radio Galaxies

We present a sample of 1317 Infrared-Faint Radio Sources (IFRSs) that, for the first time, are reliably detected in the infrared, generated by cross-correlating the Wide-Field Infrared Survey Explorer (WISE) all-sky survey with major radio surveys. Our IFRSs are brighter in both radio and infrared than the first generation IFRSs that were undetected in the infrared by the Spitzer Space Telescope. We present the first spectroscopic redshifts of IFRSs, and find that all but one of the IFRSs with spectroscopy has z > 2. We also report the first X-ray counterparts of IFRSs, and present an analysis of radio spectra and polarization, and show that they include Gigahertz-Peaked Spectrum, Compact Steep Spectrum, and Ultra-Steep Spectrum sources. These results, together with their WISE infrared colours and radio morphologies, imply that our sample of IFRSs represents a population of radio-loud Active Galactic Nuclei at z > 2. We conclude that our sample consists of lower-redshift counterparts of the extreme first generation IFRSs, suggesting that the fainter IFRSs are at even higher redshift.Comment: 23 pages, 17 figures. Submitted to MNRA