Isolation and Characterization of a New Type of Bufotoxin from the Skin of Bufo americanus

金沢大学大学院自然科学研究科分子作用学金沢大学薬学

Increased excretion of urinary cyclic GMP in primary hepatoma and preneoplastic liver.

Urinary excretion of cyclic GMP (cGMP) and the plasma level of cyclic AMP (cAMP) were determined in patients with liver diseases. The urinary excretion of cGMP, expressed on the basis of creatinine excreted per day, was at significantly higher levels not only in primary hepatoma but also in liver cirrhosis, while the plasma level of cAMP was higher only in liver cirrhosis. Thus, the ratio of urinary cGMP excretion to plasma cAMP level in primary hepatoma was significantly higher than that in liver cirrhosis. In cirrhotic patients studied by catheterization, the level of cGMP in the hepatic vein was significantly lower than that in the superior mesenteric or portal vein, indicating the uptake of cGMP by the liver. Since cGMP excretion correlated with KICG both in liver cirrhosis and primary hepatoma, the increased cGMP excretion appeared to be explained by a reduced uptake of cGMP by the liver.</p

A HIGH-PERFORMANCE LIQUID CHROMATOGRAPHIC METHOD FOR THE ASSAY OF Na^+, K^+-ADENOSINE TRIPHOSPHATASE INHIBITION

金沢大学大学院自然科学研究科分子作用学金沢大学薬学

Isolation and Characterization of New Bufotoxins from the Skin of Bufo melanostictus SCHNEIDER

金沢大学大学院自然科学研究科分子作用学金沢大学薬学

Retroviral gp70 antigen in spontaneous mesangial glomerulonephritis of ddY mice

Retroviral gp70 antigen in spontaneous mesangial glomerulonephritis of ddY mice. We examined whether the retroviral envelope antigen, gp70, is a major nephritogenic antigen in ddY mice, a murine model of spontaneous mesangial glomerulonephritis associated with IgA and IgG deposition. Immunofluorescence microscopy revealed that the mesangial gp70 deposition increased with age in mice over 24 weeks old, as did the IgG and IgA deposits. Immunoelectron microscopy demonstrated the reaction products of gp70 superimposed on the electron dense deposits in the mesangial matrix. Various amounts of serum gp70 were detected in mice as young as 12 weeks without any apparent increase with age. There was no correlation between the serum level of gp70 and the extent of the glomerular gp70 deposition, whereas mice with heavier IgA deposition had higher mean levels of serum IgA. The absorption test demonstrated that significant amounts of serum gp70 composed immune complexes in 40 week-old ddY mice developing glomerulonephritis; however, this bound form of gp70 was not observed in 12 week-old mice without glomerulonephritis. Systemic examinations by immunofluorescence staining showed that gp70 was mainly localized in various lymphoid tissues. These findings suggest that the gp70 antigen, mostly derived from lymphoid cells, may circulate as immune complexes and accumulate in the mesangial area, thus contributing to the development of glomerulonephritis in these mice. In addition, the pathogenic role of the increased IgA production in these mice was discussed

Studies on High-Performance Liquid Chromatography with Electrochemical Detection. The pH-Dependency of Enzymic Sulfation of Catechol Estrogens

金沢大学大学院自然科学研究科分子作用

ANTIGENIC PROPERTIES OF ESTRIOL 3-GLUCURONIDE-[C-6]-BOVINE SERUM ALBUMIN CONJUGATES HAVING OXIME BRIDGES

金沢大学大学院自然科学研究科分子作用学金沢大学薬学