alloantibody のマウス skin graft survival に及ぼす影響

Alloantisera (anti-donor and anti-recipient alloantiserum) were prepared by skin grafting followed four inoculations of 1×10^7 splenocytes. These alloantisera were cytotoxic (×64) to target cells specifically. Anti-recipient alloantiserum was effective on enhancing skin graft survival in mice as same as anti-donor alloantiserum, when administered on the operative day, second postoperative day and fourth postoperative day between 50-200 μl at one time

ドナー特異的 unresponsiueness を呈した腎再移植症例

A living related kidney transplant recipient, who showed very characterstic findings in immunologic study, was reported. This patient was a retransplant recipient. First graft of this patient was his mother's kidney, and the second graft was his older sister's kidney. HLA compatibility between the patient and the second donor was one haplotype identical. Although the patient was responsive in mixed-leukocyte-culture (MLC) against the second donor before first transplantation, MLC and cell-mediated-lympholysis (CML) tests before retransplantation showed specific unresponsiveness to the second donor. During 36 months after retransplantation, no rejection episodes nor any complications have been observed

アロ抗原感作および未感作リンパ球に対する低線量X線照射の効果

The effect of local graft irradiation on immune response in allograft in which acute　rejection occurs was studied using an in vitro model. Unidirectional mixed lymphocyte culture (MLC) was used as the in vitro model of acute rejection. 150 and 300 rad x-irradiation suppressed mixed lymphocyte reaction (MLR) but did not cell-mediated-lympholysis (CML) of unsensitized lymphocytes. X-irradiated alloantigen sensitized cells (ASC) generated in 6-day MLC suppressed MLR and CML of unsensitized lymphocytes. Suppressive effects of x-irradiated ASC were of the same degree by x-irradiation doses of 150-500 rad. Suppressive effect of x-irradiation was maintained for only a short period after x-irradiation. Potential function of suppressor precursor cells among unsensitized lymphocytes was abolished by x-irradiation of 300 rad

腎移植臨床例における T.G 陽性細胞の変動 : 合併症との関連性を中心に

The changes in lymphocyte subpopulations in renal transplant recipients were determined. Prior to immunosuppressive therapy, the subpopulation levels in the recipients were similar to those of healthy adults, but after administration of therapy, the T-cell ratio (%T) decreased, while the IgG-Fc receptor-bearing T-cell ratio (%T.G) increased. However, review of these changes in individual cases showed that among those with the same degree of decrease in %T, the %T.G increased only slightly in some, but markedly in others, thus, indicating that there was not necessarily an inverse correlation between the decrease in %T and increase in %T.G. Further, it is noted that when %T.G was markedly increased, the patient was susceptible to bacterial infection

Examination of Serum Class I Antigen in Allograft Recipient Rats : Origin and control of serum class I antigen

We examined the appearance of DA type (RT1Aa) class I antigen in the serum of rats that had received isogeneic or allogeneic liver grafts (DA into DA, DA into LEW, PVG into DA, PVG into F1 hybrid (DAxPVG). Recipient LEW rats were given either one injection of the anti-CD8 mAb, OX-8, following thymectomy or anti-CD4 mAb (cocktail of OX-35 and OX-38) following thymectomy 3 days prior to liver grafting. We also tested the serum RT1Aa antigen titer of F1 (DAxPVG) recipients after PVG spleen transplantation and the serum RT1Aa antigen titer in the DA rat after hepatectomy and cyclosporin treatment.　　 Replacement of DA liver by PVG lowered transiently the serum level of RT1Aa antigen to 70% of that in normal DA serum, shortly after liver transplantation. However, this titer increased gradually over the level in normal DA serum.　　 A PVG spleen graft to an F1 hybrid recipient resulted in death due to typical GVH disease between 13 and 24 days after spleen transplantation. The RT1Aa antigen titer increased to several times more than that in normal F1 serum throughout the observation period.　　 LEW recipients of DA liver died at 9-11 days (9.8± 1.1 days) due to acute liver rejection. In this combination, the serum level of RT1Aa increased until day 8, reaching a maximum (four times) on day 4. Deletion of either CD8+ or CD4+ T cells by anti-CD8 or anti-CD4 MAb in this transplantation prolonged the survival times of liver grafts for up to 26.8±8.4 and 35.6± 17.9 days, respectively. In the anti-CD8 or anti-CD4 MAb- treated recipients, the serum titer of DA class I antigen was not elevated and there were no differences between the two. Hepatectomy in combination with cyclosporin induced a high titer of liver- derived class I antigen in the serum as long as liver regeneration proceeded.　　 These results suggest that the liver is the principal source of serum class I antigen in rats. Rejection, GVH reaction and liver regeneration increased the serum class I antigen from transplanted liver or host tissue. It is unlikely that this is due to cleavage of membranous class I antigen by class I- reactive CD8+ T cells

Effect of Donor Fasting on Survival of Pancreas and Heart Grafts after Warm Ischemia

Livers from fasted animals are believed to be more vulnerable to ischemic injury than those from fed donors. However, we have recently shown the opposite: livers from fasted rats were more tolerant to ischemic injury. Indeed, the survival rate of 60 min warm ischemic damaged livers increased from 0 to 90% if donor rats were fasted for three days. In this study, we examined how donor fasting affects the outcome of pancreas and heart preservation. BN rats were used as both donors and recipients, and recipients of pancreatic grafts were rendered diabetic prior to transplantation. Pancreatic or heart grafts were subjected to 90 min or 25 min of warm ischemia and were transplanted into the right side of the necks of recipient rats. The viability rate of hearts transplanted from fed donors into fed recipients was only about 11 % (1/9) after transplantation. However, the viability rate with fasted donors was 75% (6/8). The rate of successful pancreatic grafting from fed donors into fed recipients was 28.6% (2/7), and that from fasted donors to fed recipients was 41.7% (5/12). These results confirm that the nutritional status of the donor is an important factor in the outcome of not only liver, but also pancreas and heart preservation during transplantation, although the effect of fasting on pancreatic graft is marginal

腎移植後患者の急性拒絶反応に合併した DIC

A kidney transplant recipient with disseminated intravascular coagulation (DIC) accompanied by acute rejection was described. The principal symptome of the patient was massive gross hematuria. She showed thrombocytopenia, marked decrease of fibrinogen and elevation of fibrinogen degradation products (FDP) level. The patient was treated by continuous intravenous heparin infusion (total dose was 85, 800 units), and it was very effective. The symtoms due to DIC were improved on the 9th day after the beginning of heparin therapy