18 research outputs found
PCR-based landmark unique gene (PLUG) markers effectively assign homoeologous wheat genes to A, B and D genomes-2
<p><b>Copyright information:</b></p><p>Taken from "PCR-based landmark unique gene (PLUG) markers effectively assign homoeologous wheat genes to A, B and D genomes"</p><p>http://www.biomedcentral.com/1471-2164/8/135</p><p>BMC Genomics 2007;8():135-135.</p><p>Published online 30 May 2007</p><p>PMCID:PMC1904201.</p><p></p>correspond to marker numbers indicated in Table 1. M: 2-Log DNA Ladder (New England BioLabs Inc., Ipswich, MA, USA)
PCR-based landmark unique gene (PLUG) markers effectively assign homoeologous wheat genes to A, B and D genomes-0
<p><b>Copyright information:</b></p><p>Taken from "PCR-based landmark unique gene (PLUG) markers effectively assign homoeologous wheat genes to A, B and D genomes"</p><p>http://www.biomedcentral.com/1471-2164/8/135</p><p>BMC Genomics 2007;8():135-135.</p><p>Published online 30 May 2007</p><p>PMCID:PMC1904201.</p><p></p>s database [34] and wheat UniGene data sets [43] in an interactive manner. To eliminate paralogous genes, Landmark Unique Gene loci (LUGs) were selected by pair-wise comparisons of the rice cDNA models [34]. TaEST-LUGs were selected as template loci for potential PLUG markers (see Methods). "Html1" and "Html2" are interactive interfaces where the target locus can be selected and primer picking conditions can be inputted, respectively
PCR-based landmark unique gene (PLUG) markers effectively assign homoeologous wheat genes to A, B and D genomes-3
<p><b>Copyright information:</b></p><p>Taken from "PCR-based landmark unique gene (PLUG) markers effectively assign homoeologous wheat genes to A, B and D genomes"</p><p>http://www.biomedcentral.com/1471-2164/8/135</p><p>BMC Genomics 2007;8():135-135.</p><p>Published online 30 May 2007</p><p>PMCID:PMC1904201.</p><p></p>somic lines. A-C: 1% agarose gel electrophoresis of the PCR products of Marker No. 12 (A), Marker No. 18 (B), and Marker No. 8 (C). D and E: 4% agarose gel electrophoresis of the I-digested products of Marker No. 18 (D) and III-digested products of Marker No. 8 (E)
PCR-based landmark unique gene (PLUG) markers effectively assign homoeologous wheat genes to A, B and D genomes-1
<p><b>Copyright information:</b></p><p>Taken from "PCR-based landmark unique gene (PLUG) markers effectively assign homoeologous wheat genes to A, B and D genomes"</p><p>http://www.biomedcentral.com/1471-2164/8/135</p><p>BMC Genomics 2007;8():135-135.</p><p>Published online 30 May 2007</p><p>PMCID:PMC1904201.</p><p></p>, as reported by Gale and Devos (1998) [5] and Sorrells et al. (2003) [35], is shown in different colors for each rice chromosome and wheat chromosome group
PCR-based landmark unique gene (PLUG) markers effectively assign homoeologous wheat genes to A, B and D genomes-5
<p><b>Copyright information:</b></p><p>Taken from "PCR-based landmark unique gene (PLUG) markers effectively assign homoeologous wheat genes to A, B and D genomes"</p><p>http://www.biomedcentral.com/1471-2164/8/135</p><p>BMC Genomics 2007;8():135-135.</p><p>Published online 30 May 2007</p><p>PMCID:PMC1904201.</p><p></p>s database [34] and wheat UniGene data sets [43] in an interactive manner. To eliminate paralogous genes, Landmark Unique Gene loci (LUGs) were selected by pair-wise comparisons of the rice cDNA models [34]. TaEST-LUGs were selected as template loci for potential PLUG markers (see Methods). "Html1" and "Html2" are interactive interfaces where the target locus can be selected and primer picking conditions can be inputted, respectively
PCR-based landmark unique gene (PLUG) markers effectively assign homoeologous wheat genes to A, B and D genomes-6
<p><b>Copyright information:</b></p><p>Taken from "PCR-based landmark unique gene (PLUG) markers effectively assign homoeologous wheat genes to A, B and D genomes"</p><p>http://www.biomedcentral.com/1471-2164/8/135</p><p>BMC Genomics 2007;8():135-135.</p><p>Published online 30 May 2007</p><p>PMCID:PMC1904201.</p><p></p>, as reported by Gale and Devos (1998) [5] and Sorrells et al. (2003) [35], is shown in different colors for each rice chromosome and wheat chromosome group
Using the Hexaploid Nature of Wheat To Create Variability in Starch Characteristics
In hexaploid crops, such as bread
wheat, it should be possible
to fine-tune phenotypic traits by identifying wild-type and null genes
from each of the three genomes and combining them in a calculated
manner. Here, we demonstrate this with gene combinations for two starch
synthesis genes, <i>SSIIa</i> and <i>GBSSI</i>. Lines with inactive copies of both enzymes show a very dramatic
change in phenotype, so to
create intermediate phenotypes, we used marker-assisted selection
to develop near-isogenic lines (NILs) carrying homozygous combinations
of null alleles. For both genes, gene dosage effects follow the order
B > D ≥ A; therefore, we completed detailed analysis of
starch
characteristics for NIL 3-3, which is null for the B-genome copy of
the <i>SSIIa</i> and <i>GBSSI</i> genes, and NIL
5-5, which has null mutations in the B- and D-genome-encoded copies
of both of these genes. The effects of the combinations on phenotypic
traits followed the order expected on the basis of genotype, with
NIL 5-5 showing the largest differences from the wild type, while
NIL 3-3 characteristics were intermediate between NIL 5-5 and the
wild type. Differences among genotypes were significant for many starch
characteristics, including percent amylose, chain length distribution,
gelatinization temperature, retrogradation, and pasting properties,
and these differences appeared to translate into improvements in end-product
quality, since bread made from type 5-5 flour showed a 3 day lag in
staling
Correlation coefficients among environments for flour yield (FlYd).
<p>Correlation coefficients among environments for flour yield (FlYd).</p
Relationships between flour yield (FlYd) and flour efficiency (FlEf) (A), FlYd and median diameter of particles (x50) (B) and FlYd and specific surface area of particles (Sv) (C).
<p>Accessions could be classified into either soft or hard kernel types based on <i>Pina-D1</i>/<i>Pinb-D1</i> genotypes. Soft accessions have <i>Pina-D1a</i> and <i>Pinb-D1a</i>, while hard have <i>Pina-D1b</i> or <i>Pinb-D1b</i>.</p
PCR assays to detect of polymorphic SNPs between Kitahonami and the three other varieties used as parents in the DH populations.
<p>White and black arrows indicate bands derived from genome-specific and allele-specific amplicons, respectively. 1: Kitahonami, 2: Kinuhime, 3: Tohoku224, 4: Shunyou.</p