Effect of VEGF121 or VEGF165 on the cell shape and F-actin structure in TM cells.

<p>VEGF121 or VEGF165 were used to treat TM cells for 48 h. The top panels show the phase contrast images and the bottom panels show the F-actin-stained images (red). Scale bar, 100 μm.</p

The Effect of VEGF165 on TEER in TM Cells.

<p>The Effect of VEGF165 on TEER in TM Cells.</p

The Effect of VEGF121 on TEER in SCE Cells.

<p>The Effect of VEGF121 on TEER in SCE Cells.</p

Effect of VEGF121 or VEGF165 on TEER in TM cell monolayers.

<p>TM cells were treated with VEGF121 (A, B) or VEGF165 (C, D) for 48 h. (A, C) The time course of TEER changes after 30 ng/mL VEGF121 (A) or VEGF165 (C) treatment. (B, D) TEER changes after VEGF121 (B) or VEGF165 (D) treatment for 48 h. Data are expressed as the mean ± SD from six separate filters (n = 6).</p

Effect of perfusion with VEGF121 on outflow facility.

<p>The anterior segments of porcine eyes were perfused with 30 ng/mL VEGF121 at a constant flow of 3 μL/min at 37°C for 48 h. Data of outflow facility are expressed as the percent change from the baseline value, and expressed as the mean (n = 5).</p

The Effect of VEGF165 on TEER in SCE Cells.

<p>The Effect of VEGF165 on TEER in SCE Cells.</p

Effect of VEGF receptor inhibitors on TEER in SCE cell monolayers.

<p>SCE cells were treated with VEGF121 with or without axitinib (A), Ki8751 (B), or ZM306416 (C) for 48 h. Data are expressed as the mean ± SD from six (A, B) or eight (C) separate filters. *<i>P</i> < 0.05 and **<i>P</i> < 0.01 using the Tukey–Kramer HSD test.</p

The Effect of VEGF121 on Outflow Facility in Porcine Eyes.

<p>The Effect of VEGF121 on Outflow Facility in Porcine Eyes.</p

Effect of VEGF121 or VEGF165 on cell shape, F-actin structure, and junctional proteins in SCE cells.

<p>VEGF121 or VEGF165 were used to treat SCE cells for 48 h. The top panels show the phase contrast images and the middle panels show the F-actin-stained images (red). Cell nuclei were counterstained with 4',6-diamidino-2-phenylindole (DAPI, blue, middle panel). Bottom panels show a related molecule (ZO-1, green) involved in cell-cell contact. Scale bar, 100 μm.</p

Correlation between the level of TGF-β2 and those of MCP-1 and TNF-α.

<p>Scatter plots of the levels of TGF-β2, MCP-1 (A) and TNF-α (B) in aqueous humor of UG (<i>n</i> = 14). The level of TGF-β2 negatively correlated with the levels of MCP-1 (adjusted R² = 0.28, t = -2.45, P = 0.031) and TNF-α (adjusted R² = 0.27, t = -2.43, P = 0.032).</p