Coup in the coop: Rank changes in chicken dominance hierarchies over maturation.

Chicken dominance hierarchies or pecking orders are established before maturation and maintained by consistent submissive responses of subordinate individuals, leading to stable ranks within unchanged groups. We observed interactions of 418 laying hens (Gallus gallus domesticus) distributed across three small (20) and three large (~120) groups. The observations were performed before sexual maturation (young period) and additionally after onset of maturation (mature period) to confirm stability of ranks. Dominance ranks were estimated via the Elo rating system across both observation periods. Diagnostics of the ranks revealed unexpected uncertainty and rank instability for the full dataset, although sampling appeared to be adequate. Subsequent evaluations of ranks based on the mature period only, showed more reliable ranks than across both observation periods. Furthermore, winning success during the young period did not directly predict high rank during the mature period. These results indicated rank changes between observation periods. The current study design could not discern whether ranks were stable in all pens before maturation. However, our data rather suggested active rank mobility after hierarchy establishment to be the cause for our findings. Once thought to be stable, chicken hierarchies may provide an excellent system to study causes and implications of active rank mobility

Commercial hatchery practices have long-lasting effects on laying hens' spatial behaviour and health.

The commercial hatchery process is globally standardized and exposes billions of day-old layer chicks to stress every year. By alleviating this early stress, on-farm hatching is thought to improve animal welfare, yet little is known about its effects throughout production. This study compared welfare indicators and spatial behaviours during the laying period of hens hatched in an on-farm environment (OFH) to those hatched in a commercial hatchery and transferred at one day-old to a rearing barn (STAN). In particular, we assessed how OFH and TRAN hens differed in space-use and movement behaviours following the transfer to the laying barn at 17 weeks of age, a similar stressor encountered by STAN hens early in life, and determined whether effects aligned more with the 'silver-spoon' or 'environmental matching' hypothesis. We found that for the first three months post-transfer into the laying barn, OFH hens, on average, transitioned less between the aviary's tiers and spent less time on the littered floor. Because OFH hens became behaviourally more similar to STAN hens over time, these results suggest that OFH hens required a prolonged period to establish their daily behavioural patterns. Furthermore, OFH hens had more severe keel bone fractures throughout the laying period but similar feather damage and body mass to STAN hens. No differences were found in hen mortality or the number of eggs per live hen. These findings support the environmental matching hypothesis and suggest that early-life stressors may have prepared hens for later-life stressors, underscoring the importance of both early-life and adult environments in enhancing animal welfare throughout production

Evaluation of an Active LF Tracking System and Data Processing Methods for Livestock Precision Farming in the Poultry Sector

Tracking technologies offer a way to monitor movement of many individuals over long time periods with minimal disturbances and could become a helpful tool for a variety of uses in animal agriculture, including health monitoring or selection of breeding traits that benefit welfare within intensive cage-free poultry farming. Herein, we present an active, low-frequency tracking system that distinguishes between five predefined zones within a commercial aviary. We aimed to evaluate both the processed and unprocessed datasets against a “ground truth” based on video observations. The two data processing methods aimed to filter false registrations, one with a simple deterministic approach and one with a tree-based classifier. We found the unprocessed data accurately determined birds’ presence/absence in each zone with an accuracy of 99% but overestimated the number of transitions taken by birds per zone, explaining only 23% of the actual variation. However, the two processed datasets were found to be suitable to monitor the number of transitions per individual, accounting for 91% and 99% of the actual variation, respectively. To further evaluate the tracking system, we estimated the error rate of registrations (by applying the classifier) in relation to three factors, which suggested a higher number of false registrations towards specific areas, periods with reduced humidity, and periods with reduced temperature. We concluded that the presented tracking system is well suited for commercial aviaries to measure individuals’ transitions and individuals’ presence/absence in predefined zones. Nonetheless, under these settings, data processing remains a necessary step in obtaining reliable data. For future work, we recommend the use of automatic calibration to improve the system’s performance and to envision finer movements

Tracking performance in poultry is affected by data cleaning method and housing system

Sensor-based behavioural observation methods improve our understanding of individual behaviour and welfare in large commercial groups, including poultry. Validating automatically generated data is essential to account for potential sources of error. Our study aimed to validate a sensor-based tracking system for broiler breeders (BB) and laying hens (LH) in commercially relevant housing systems. The BB study was conducted in 10 pens with 33 females and three males (Ross 308) per pen. Half of the pens contained a raised slatted area and two raised group nests (Raised), while in the remaining five pens, the nests and slats were on the floor (Floor). For the LH study, six pens with a commercial aviary were used, with 225 Dekalb White hens housed per pen (Aviary). Focal hens (BB, 10/pen; LH, 18/pen) were equipped with backpacks containing tracking devices that registered transitions between four (BB) or five (LH) resource-related zones covering all accessible areas within each housing system. The tracking data was compared against video observations for 20 focal BB on two days and 18 focal LH on three days (3 × 20 min/day). Three data cleaning methods tested with 30 values of a duration parameter were evaluated for reliability and stability with a cross-validation approach. Initial and post-cleaning performance were assessed with accuracy, precision, and sensitivity of recorded transitions and by calculating the reliability for two aspects of movement: total transitions (Lin’s Concordance Correlation Coefficient) and locations (mean proportion of matching duration). A mixed model was applied to evaluate the duration of stay after false and true tracking registrations. Initial location reliability was high (> 0.949) in all housing systems, while reliability of total transitions was low ( 0.832) while reliability of locations remained high (> 0.949) in Aviary and Raised. The duration between registrations was affected by housing system (p < 0.001) and was longer for true compared to false registrations (p < 0.001). Initial tracking performance varied between movement aspects and housing systems. The difference in duration between true and false registrations allowed for the application of simple yet effective data cleaning in Aviary and Raised, ensuring that the generated data better represented the animal's actual movement with reduced error associated with the tracking system

Effects of on-farm hatching on short term stress indicators, weight gain, and cognitive ability in layer chicks

Layer chicks are usually transported early in life, experiencing immediate post-hatch food and water deprivation and various transport-related stressors with potentially negative long-term consequences for learning, cognition and welfare. In contrast, as chicks are only temporarily exposed to these stressors, the experienced stress could be sub-chronic which may improve cognitive flexibility. The aim of this exploratory study was therefore to investigate the acute and long-term effects of on-farm hatching (OFH) compared to conventional hatching. Dekalb White layer chicks were subjected to either OFH (n = 47) with ad libitum access to feed and water or temporary post-hatch resource deprivation and eight hour transport (RDT; n = 42). Physical and behavioural measures were collected to examine short-term effects of the treatment procedures. To determine longer term effects, treatment differences in learning and cognitive flexibility were assessed in a Y-maze using several paradigms (reversal, attentional-shift, extinction) between 4 and 12 weeks of age (WOA). Compared to OFH chicks, RDT had: greater corticosterone levels after transport (F1,19 =8.15, p = 0.01, RDT (16.24 ± 1.20 ng/mL) vs. OFH (8.13 ± 1.20 ng/mL) and post-recovery (F1,19 =4.93, p = 0.04; RDT (11.69 ± 1.35 ng/mL) vs. OFH (5.31 ± 1.37)), and lower body mass after resource deprivation and transport (F2258 =9.7, p < 0.001, RDT (33.14 ± 0.33 g) vs. OFH (37.62 ± 0.28 g)). Performance of activity behaviours (foraging, drinking, resting, wing-assisted running) after transport exhibited treatment by time interactions. Additionally, a tendency for OFH being heavier than RDT chicks was observed up to 11 WOA. The majority of birds learned the initial association in the Y-maze between a reward and location (77% of n = 19 RDT and n = 29 OFH chicks) or light stimulus (91% of n = 12 RDT and n = 11 OFH chicks). Subsequently, a number of chicks reached the learning criterion in the location reversal (24% of n = 13 RDT and n = 24 OFH chicks) and the light-to-location attentional-shift (47% of n = 11 RDT and n = 10 OFH chicks), and most of these chicks succeeded in the following extinction paradigm (80% of n = 3 RDT and n = 7 OFH chicks). No treatment effects were detected in any phase of cognitive testing. In conclusion, treatment affected behaviour and health parameters suggesting RDT animals were recovering from resource deprivation and transport. Continued treatment differences in body mass throughout rearing demonstrated long term effects as well although no effects on initial learning and cognitive flexibility were identified. Future work is needed to determine what mechanisms are responsible for the observed health and behavioural differences

Intra-individual variation of hen movements is associated with later keel bone fractures in a quasi-commercial aviary.

Measuring intra- and inter-individual variation in movement can bring important insights into the fundamental ecology of animals and their welfare. Although previous studies identified consistent differences in movements of laying hens within commercial aviaries, the level of consistency was not quantified, limiting our capacity to understand the importance of individual movements for welfare. We aimed to quantify the scope of intra- and inter-individual differences in movements of commercial laying hens and examined their associations with indicators of welfare at the end of production. We quantified individual differences in one composite daily movement score for 80 hens over 54 days post-transfer to a quasi-commercial aviary. Results showed consistent inter-individual differences in movement averages, explaining 44% of the variation, as well as individual variation in predictability and temporal plasticity (at the population-level, hens increased their movements for 39 days). Hens that were more predictable in their daily movements had more severe keel bone fractures at the end of production while we found no such correlation between daily movement averages (individual intercept) and welfare indicators. Our findings highlight the importance of inter-individual difference in intra-individual variation of movements to improve poultry welfare

Keel bone fractures induce a depressive-like state in laying hens

In commercial flocks of laying hens, keel bone fractures (KBFs) are prevalent and associated with behavioural indicators of pain. However, whether their impact is severe enough to induce a depressive-like state of chronic stress is unknown. As chronic stress downregulates adult hippocampal neurogenesis (AHN) in mammals and birds, we employ this measure as a neural biomarker of subjective welfare state. Radiographs obtained longitudinally from Lohmann Brown laying hens housed in a commercial multi-tier aviary were used to score the severity of naturally-occurring KBFs between the ages of 21–62 weeks. Individual birds’ transitions between aviary zones were also recorded. Focal hens with severe KBFs at 3–4 weeks prior to sampling (n = 15) had lower densities of immature doublecortin-positive (DCX+) multipolar and bipolar neurons in the hippocampal formation than focal hens with minimal fractures (n = 9). KBF severity scores at this time also negatively predicted DCX+ cell numbers on an individual level, while hens that acquired fractures earlier in their lives had fewer DCX+ neurons in the caudal hippocampal formation. Activity levels 3–4 weeks prior to sampling were not associated with AHN. KBFs thus lead to a negative affective state lasting at least 3–4 weeks, and management steps to reduce their occurrence are likely to have significant welfare benefits

Influence of keel impacts and laying hen behavior on keel bone damage.

Keel bone damage, which presents as fractures and/or deviations of the keel, has been detected in laying hens housed in all types of systems. Factors leading to keel bone damage in hens housed with limited vertical space, such as those housed in furnished systems, are not well understood, and are the topic of this study. Ten focal hens from each of 12 furnished cages (4 rooms of 3 cages) were fitted with keel mounted tri-axial accelerometers. Their behavior was video recorded continuously over two 3-wk trials: the first when the hens were between 52 and 60 wk of age, and the second approximately 20 wk later. The integrity of each hen's keel was evaluated at the start and end of each 3-wk trial using digital computed tomography. We identified predominant behaviors associated with acceleration events sustained at the keel (collisions, aggressive interactions and grooming) by pairing accelerometer outputs with video data. For each recorded acceleration event we calculated the acceleration magnitudes as the maximum summed acceleration recorded during the event, and by calculating the area under the acceleration curve. A principle components analysis, which was used as a data reduction technique, resulted in the identification of 4 components that were used in a subsequent regression analysis. A key finding is that the number of collisions a hen has with structures in her environment, and the number of aggressive interactions that a hen is involved, each affect the likelihood that she will develop 1 or more fractures within a 3-wk time span. This relationship between hen behavior and keel fracture formation was independent of the magnitude of acceleration involved in the event. Observed behavior did not have an impact on the formation of keel bone deviations, further supporting reports that the mechanisms underlying the 2 types of keel bone damage are different

Skeletal variation in bird domestication: limb proportions and sternum in chicken, with comparisons to mallard ducks and Muscovy ducks

Background Domestication, including selective breeding, can lead to morphological changes of biomechanical relevance. In birds, limb proportions and sternum characteristics are of great importance and have been studied in the past for their relation with flight, terrestrial locomotion and animal welfare. In this work we studied the effects of domestication and breed formation in limb proportions and sternum characteristics in chicken (Gallus gallus), mallard ducks (Anas plathyrhynchos) and Muscovy ducks (Cairina moschata). Methods First, we quantified the proportional length of three long bones of the forelimb (humerus, radius, and carpometacarpus) and the hind limb (femur, tibiotarsus, and tarsometatarsus) in domestic chickens, mallard ducks, and Muscovy ducks and their wild counterparts. For this, we took linear measurements of these bones and compared their proportions in the wild vs. the domestic group in each species. In chicken, these comparisons could also be conducted among different breeds. We then evaluated the proportional differences in the context of static and ontogenetic allometry. Further, we compared discrete sternum characteristics in red jungle fowl and chicken breeds. In total, we examined limb bones of 287 specimens and keel bones of 63 specimens. Results We found a lack of significant change in the proportions of limb bones of chicken and Muscovy duck due to domestication, but significant differences in the case of mallard ducks. Variation of evolvability, allometric scaling, and heterochrony may serve to describe some of the patterns of change we report. Flight capacity loss in mallard ducks resulting from domestication may have a relation with the difference in limb proportions. The lack of variation in proportions that could distinguish domestic from wild forms of chicken and Muscovy ducks may reflect no selection for flight capacity during the domestication process in these groups. In chicken, some of the differences identified in the traits discussed are breed-dependent. The study of the sternum revealed that the condition of crooked keel was not unique to domestic chicken, that some sternal characteristics were more frequent in certain chicken breeds than in others, and that overall there were no keel characteristics that are unique for certain chicken breeds. Despite some similar morphological changes identified across species, this study highlights the lack of universal patterns in domestication and breed formation