Characterization of a gene coding for a putative adenosine deaminase-related growth factor by RNA interference in the Basidiomycete Flammulina velutipes

A full-length cDNA coding for a putative adenosine deaminase (Fv-ada) was isolated from the basidiomycete Flammulina velutipes. Fv-ada encodes a polypeptide consisting of 537 amino acid residues, which has a consensus sequence conserved among adenosine deaminase-related growth factors (ADGF) found in several metazoa, including chordates and insects. Fv-ada transcript was detected at all stages of growth in dikaryotic F. velutipes cells, with a peak at the primordial stage. Heterologous expression of Fv-ada in the yeast Pichia pastoris produced recombinant Fv-ADA that catalyzed the conversion of adenosine to inosine. Dikaryotic mycelia from F. velutipes were transformed with the binary plasmid pFungiway-Fv-ada, which was designed to suppress the expression of Fv-ada through RNA interference. The growth rates of the resulting transformants were retarded in response to the degree of suppression, indicating that Fv-ada plays an important role in the mycelial growth of F. velutipes. These results suggested that ADGF could function as growth factors in fungi, as is seen in other eukaryotes.ArticleJOURNAL OF BIOSCIENCE AND BIOENGINEERING. 115(4):360-365 (2013)journal articl

Subaru Observations for the K-band Luminosity Distribution of Galaxies in Clusters near to 3C 324 at z∼\sim1.2

We investigate the $K$-band luminosity distribution of galaxies in the region of clusters at $z\sim1.2$ near to the radio galaxy 3C 324. The imaging data were obtained during the commissioning period of the Subaru telescope. There is a significant excess of the surface number density of the galaxies with $K =$ 17--20 mag in the region within $\sim$ 40'' from 3C 324. At this bright end, the measured luminosity distribution shows a drop, which can be represented by the exponential cut off of the Schechter-function formula; the best-fitted value of the characteristic magnitude, $K^{*}$, is $\sim 18.4\pm0.8$. This measurement follows the evolutionary trend of the $K^*$ of the rich clusters observed at an intermediate redshift, which is consistent with passive evolution models with a formation redshift z_f \gtsim 2. At K \gtsim 20 mag, however, the excess of the galaxy surface density in the region of the clusters decreases abruptly, which may imply that the luminosity function of the cluster galaxies has a negative slope at the faint end. This may imply strong luminosity segregation between the inner and outer parts of the clusters, or some deficit of faint galaxies in the cluster central region of the cluster.Comment: 12 pages, 9 figures, accepted for publication in PAS

High-Resolution Near-Infrared Imaging of the Powerful Radio Galaxy 3C 324 at z = 1.21 with the Subaru Telescope

We have obtained high-resolution K'-band images of the powerful z=1.206 radio galaxy 3C 324 with the Subaru telescope under seeing conditions of 0.3--0.4 arcsec. We clearly resolved the galaxy and directly compared it to the optical images obtained with the Hubble Space Telescope. The host galaxy of 3C 324 is revealed to be a moderately luminous elliptical galaxy with a smooth light profile. The effective radius of the galaxy, as determined by profile fitting, is 1.3+-0.1 arcsec (1.2 kpc), which is significantly smaller than the value of 2.2 arcsec, published in Best et al. (1998, MNRAS, 292, 758). The peak of the K'-band light coincides with the position of the radio core, which implies that the powerful AGN lies at the nucleus of the host galaxy. The peak also coincides with the gap in the optical knotty structures which may be a dust lane hiding the UV-optical emission of the AGN from our line of sight; it is very likely that we are seeing the obscuring structure almost edge-on. We clearly detected the `aligned component' in the K'-band image by subtracting a model elliptical galaxy from the observed image. The red R_F702W-K color of the outer region of the galaxy avoiding the aligned component indicates that the near infrared light of the host galaxy is dominated by an old stellar population.Comment: 21 pages (10 figures), accepted for publication in PAS