Large-Scale Structure -Function Analysis of the Arabidopsis RPM1 Disease Resistance Protein

The Arabidopsis RPM1 gene confers resistance against Pseudomonas syringae expressing either the AvrRpm1 or the AvrB type III effector protein. We present an exhaustive genetic screen for mutants that no longer recognize avrRpm1. Using an inducible avrRpm1 expression system, we identified 110 independent mutations. These mutations represent six complementation groups. None discriminates between avrRpm1 and avrB recognition. We identified 95 rpm1 alleles and present a detailed structure–function analysis of the RPM1 protein. Several rpm1 mutants retain partial function, and we deduce that their residual activity is dependent on the level of avrRpm1 signal. In these mutants, the hypersensitive response remains activated if the signal goes above a certain threshold. Missense mutations in rpm1 are highly enriched in the nucleotide binding domain, suggesting that this region plays a key role either in the hypersensitive response associated with RPM1 activation or in RPM1 stability. Cluster analysis of rpm1 alleles defines functionally important residues that are highly conserved between nucleotide binding site leucine-rich repeat R proteins and those that are unique to RPM1. Regions of RPM1 to which no loss-of-function alleles map may represent domains in which variation is tolerated and may contribute to the evolution of new R gene specificities

Acoustic assessment and distribution of the main pelagic fish species in ICES Subdivision 9a South during the ECOCADIZ-RECLUTAS 2018-10 Spanish survey (October 2017).

The present working document summarises the main results obtained during the ECOCADIZ-RECLUTAS 2018-10 Spanish (pelagic ecosystem-) acoustic survey. The survey was conducted by IEO between 10th and 29th October 2018 in the Portuguese and Spanish shelf waters (20-200 m isobaths) off the Gulf of Cadiz onboard the R/V Ramón Margalef. The survey’s main objective is the acoustic assessment of anchovy and sardine juveniles (age 0 fish) in the recruitment areas of the Gulf of Cadiz. The 21 foreseen acoustic transects were sampled. A total of 25 valid fishing hauls were carried out for echo-trace ground-truthing purposes. Chub mackerel was the most frequent species in those hauls, followed by sardine, anchovy, horse mackerel, mackerel, bogue and Mediterranean horse mackerel. Acoustic sampling was carried out with the recently installed Simrad™ EK80 echo-sounder working in multi-frequency and in CW mode. A misconfiguration of the range of the acoustic active layer entailed to slow down the ping rate (1.5-2.0 seconds) in relation to the standard values (at about 0.3 seconds), resulting an acoustic sampling rate much lower than it should be. Therefore, the results from this acoustic sampling and the resulting estimates from this survey should be considered with caution. Anchovy abundance and biomass were of 953 million fish and 10 493 t. The abundance and biomass of age-0 anchovies were estimated at 543 million fish and 3 834 t, 57% and 36% of the total population abundance and biomass, respectively. Despite the methodological problems, these estimates seem to suggest a recent decrease in relation to previous years. The estimates for Gulf of Cadiz sardine in the surveyed area were of 1 134 million fish and 20 679 t. Estimates of age-0 sardine were of 1 036 million fish and 15 224 t, 91% and 74% of the total estimated abundance and biomass, respectively. Even taking into account a possible underestimation for the abovementioned methodological problems, the values reached in 2018 were above the historical mean for the total population and recruits abundance and for the recruit biomass, and they might suggest a relatively stable situation since the maxima registered in 2016

Acoustic assessment and distribution of the main pelagic fish species in ICES Subdivision 9a South during the ECOCADIZ-RECLUTAS 2017-10 Spanish survey (October 2017).

The present working document summarises the main results obtained during the ECOCADIZ‐RECLUTAS 2017‐10 Spanish (pelagic ecosystem‐) acoustic survey. The survey’s main objective is the acoustic assessment of anchovy and sardine juveniles (age 0 fish) in the recruitment areas of the Gulf of Cadiz. The survey was planned to be conducted by IEO between 12nd and 31st October 2017 in the Portuguese and Spanish shelf waters (20‐200 m isobaths) off the Gulf of Cadiz onboard the R/V Ramón Margalef. However, a serious breakdown of the vessel’s propulsion system detected in the afternoon of the 22th October led to the early termination of the survey in that day. Only the seven (7) easternmost acoustic transects were sampled, which were accompanied by the conduction of eight (8) fishing hauls. The resulting estimates are therefore referred to this surveyed area, which corresponded to the eastern sector of the Spanish shelf waters, comprising the shelf between Doñana and Cape Trafalgar, and they are not comparable to the previous available estimates. Anchovy abundance and biomass in that surveyed area were 1 492 million fish and 7 641 t. The abundance and biomass of age 0 anchovies in the surveyed area were estimated at 1 433 million fish and 7 290 t. This juvenile fraction accounted for 96% and 95% of the total estimated population abundance and biomass, respectively. The estimates for Gulf of Cadiz sardine in the surveyed area were of 591 million fish and 12 103 t. Estimates of age‐0 sardine were of 483 million fish and 8 778 t, 82% and 72% of the total population, respectively. These estimates cannot be compared with the remaining data points in the series because the abovementioned problems with acoustic sampling coverage

Human-robot cooperation for robust surface treatment using non-conventional sliding mode control

© 2018 ISA This work presents a human-robot closely collaborative solution to cooperatively perform surface treatment tasks such as polishing, grinding, deburring, etc. The method considers two force sensors attached to the manipulator end-effector and tool: one sensor is used to properly accomplish the surface treatment task, while the second one is used by the operator to guide the robot tool. The proposed scheme is based on task priority and adaptive non-conventional sliding mode control. The applicability of the proposed approach is substantiated by experimental results using a redundant 7R manipulator: the Sawyer cobot