Large-scale analysis of full-length cDNAs from the tomato (Solanum lycopersicum) cultivar Micro-Tom, a reference system for the Solanaceae genomics

<p>Abstract</p> <p>Background</p> <p>The Solanaceae family includes several economically important vegetable crops. The tomato (<it>Solanum lycopersicum</it>) is regarded as a model plant of the Solanaceae family. Recently, a number of tomato resources have been developed in parallel with the ongoing tomato genome sequencing project. In particular, a miniature cultivar, Micro-Tom, is regarded as a model system in tomato genomics, and a number of genomics resources in the Micro-Tom-background, such as ESTs and mutagenized lines, have been established by an international alliance.</p> <p>Results</p> <p>To accelerate the progress in tomato genomics, we developed a collection of fully-sequenced 13,227 Micro-Tom full-length cDNAs. By checking redundant sequences, coding sequences, and chimeric sequences, a set of 11,502 non-redundant full-length cDNAs (nrFLcDNAs) was generated. Analysis of untranslated regions demonstrated that tomato has longer 5'- and 3'-untranslated regions than most other plants but rice. Classification of functions of proteins predicted from the coding sequences demonstrated that nrFLcDNAs covered a broad range of functions. A comparison of nrFLcDNAs with genes of sixteen plants facilitated the identification of tomato genes that are not found in other plants, most of which did not have known protein domains. Mapping of the nrFLcDNAs onto currently available tomato genome sequences facilitated prediction of exon-intron structure. Introns of tomato genes were longer than those of Arabidopsis and rice. According to a comparison of exon sequences between the nrFLcDNAs and the tomato genome sequences, the frequency of nucleotide mismatch in exons between Micro-Tom and the genome-sequencing cultivar (Heinz 1706) was estimated to be 0.061%.</p> <p>Conclusion</p> <p>The collection of Micro-Tom nrFLcDNAs generated in this study will serve as a valuable genomic tool for plant biologists to bridge the gap between basic and applied studies. The nrFLcDNA sequences will help annotation of the tomato whole-genome sequence and aid in tomato functional genomics and molecular breeding. Full-length cDNA sequences and their annotations are provided in the database KaFTom <url>http://www.pgb.kazusa.or.jp/kaftom/</url> via the website of the National Bioresource Project Tomato <url>http://tomato.nbrp.jp</url>.</p

Measurement of very forward particle production at RHIC with √s=510 GeV proton-proton collisions

The Relativistic Heavy Ion Collider forward (RHICf) experiment has measured neutral particles produced in the very forward direction in the √s=510 GeV proton-proton collisions at RHIC in June 2017. The production cross sections of these particles are crucial to understand the hadronic interaction relevant to the air shower development at the cosmic-ray equivalent energy of 1.4×10$^{14}$ eV, just below the energy of the knee. Together with the data at LHC, accelerator data can cover the interaction in the cosmic-ray energy of 10$^{14}$ eV to 10$^{17}$ eV. In addition, RHICf is able to improve the former measurements of single-spin asymmetry in the polarized proton- proton collisions that is sensitive to the fundamental process of the meson exchange. Common data taking with the STAR experiment will shed light on the unexplored low mass diffraction process

Case with a Nonreassuring Fetal Status Induced by Massive Hematemesis due to Mallory-Weiss Tear That Required Emergency Cesarean Section at 38 Weeks’ Gestation

We describe a rare case of Mallory-Weiss tear with massive hematemesis at 38 weeks’ gestation. A 35-year-old woman presented with epigastralgia followed by massive hematemesis. An emergency endoscopy indicated active pulsatile bleeding at the esophagocardial junction. Although an emergency endoscopic hemostasis was successful, late decelerations without acceleration on cardiotocogram were observed. Therefore, the patient underwent emergency cesarean section, along with blood transfusion, following the endoscopic hemostasis. The hemoglobin level just before the operation was 5.1 g/dL. We suspected that massive hematemesis induced maternal acute anemia and hypovolemia, which resulted in a nonreassuring fetal status. Hence, urgent endoscopic hemostasis, adequate blood transfusion, and emergency cesarean section were needed. Mallory-Weiss tear during the third trimester may have a possibility of massive hematemesis and urgent blood transfusion, emergency endoscopic hemostasis, and emergency cesarean section may be needed

Body measurement of riding horses with a versatile tablet-type 3D scanning device

The measurement of various body dimensions of horses plays a significant role in quality improvement, genetic breeding, health, and soundness. There has been significant advancement in the technology for acquiring stereoscopic images with a three-dimensional (3D) scanner. This study aimed to validate the accuracy of body measurements obtained from stereoscopic images taken with a 3D scanner. We manually took the following body measurements for 8 riding horses: height at the withers, height at the back, height at the croup, chest depth, width of the chest, width of the croup, width of the waist, girth circumference, cannon circumference, and body length. Using a versatile tablet-type 3D scanning device, we captured a 3D image of each horse. Relative errors varied from −1.37% to 6.25%. The correlation coefficient between manual and 3D measurements was significant for all body measurements (P<0.01) except for width of the waist and cannon circumference. The low accuracy of cannon circumference (r=0.248) was due to effect of hair. A simple regression analysis of all body measurements revealed a strong correlation (P<0.001, R2=0.9994, root-mean-square error [RMSE]=1.522). Notable advantages of this methodology include high accuracy, good operability, non-contact, high versatility, and low cost. Further studies are required for the establishment of an accurate measurement methodology that can scan the whole body in a shorter time

Latest results from the LHCf Experiment

The LHC forward (LHCf) is a small experiment at the LHC designed to measure neutral particles emitted in the very forward region (pseudo-rapidity h > 8 : 4). It has measured proton–proton col- lisions at p s = 0 : 9 ; 2 : 76 ; 7 and 13 TeV, and proton–lead collisions at p s NN = 5 : 02 and 8.16 TeV. Its main purpose is to provide precise measurements of the production spectra of these forward particles, in order to calibrate hadronic interaction models used in air shower observations. In this paper we will present the current status of the LHCf experiment, regarding in particular collected data and analysis results, as well as future prospects

Status of the LHCf experiment

International audienceThe LHCf experiment is an LHC experiment dedicated to measure the production spectra of forward neutral particles, photons, π0’s, and neutrons. The aim of the LHCf is to provide critical data to test and tune hadronic interaction models which are used in MC simulations for cosmic-ray air shower developments. The LHCf had an operation in 2015 with pp collisions at $\sqrt{s}$ = 13 TeV, which corresponds to the collision energy of $0.9×10^{17}$eV in the laboratory frame. The resent results of the LH√Cf, the inclusive energy spectra for forward photons and neutrons obtained with pp collisions at $\sqrt{s}$ = 13 TeV, are presented. In addition, future prospects of LHCf analyses and activities are reviewed

ESD Rice Project: The trial of cultivation and succession in the schoolgarden of the ancient rice and tradition rice as a cultural heritage

奈良教育大学はASP UnivNetに加盟して地域の拠点校となっている。この加盟大学が地域の学校を支援するかたちでRice Projectが始まり、その連携プロジェクトとして学附協同で県内産をはじめとした古代米ならびに高齢化による伝承米の後継者不足へのサポート対策として学校園での栽培継承を試みた。本年度は初年度の試みとして、年間を通した活動の流れを記録する中で、米プロジェクトのESD的意味付けを考察し、古代米・伝承米を文化遺産として学校園で引き継ぐことが、持続可能な社会づくりにどのように位置づけられるかにも言及した