Effect of compound 4f on (A) Collagen induced protein phosphorylation (B) A23187 induced γ-glutamyltransferase activity (C) MTT cell viability assay (D) LDH release in platelets.

<p>(A) Lane I- resting platelets (untreated). Lane II- platelets treated with Collagen (1 µg/mL). Lanes III, IV and V- pre-loaded platelets with collagen and incubated with 4f in increasing concentration of 25, 50 and 100 µM respectively. Values are presented as mean ± SEM (n = 5). ***<i>p</i><0.001; significant compared to control. ^#<i>p</i><0.05, ^##<i>p</i><0.01, ^###<i>p</i><0.001; significant compared to agonist.</p

Efficacy of Ibuprofen and its derivatives measured in terms of (A) Reduction potential (B) DPPH radical scavenging activity in comparison with Quercetin.

<p>Values are presented as mean ± SEM (n = 5).</p

Effect of compound 4f on A23187 induced (A) Translocation of cytosolic cytochrome C and activation of caspase-3 (B) Caspase-9 and (C) Caspase-3 activities and (D) PS externalization in PRP and washed platelets.

<p>Values are presented as mean ± SEM (n = 5), expressed as percentage increase in (B & C) caspase activity and (D) Annexin V-FITC fluorescence and expressed as percentage increase in apoptotic platelets expressing PS relative to control. ***<i>p</i><0.001; significant compared to control. ^#<i>p</i><0.05, ^##<i>p</i><0.01, ^###<i>p</i><0.001; significant compared to A23187.</p

Effect of compound 4f on A23187 induced (A) Endogenous generation of ROS (B) Intracellular calcium levels (C) Mitochondrial membrane depolarization and (D) Peroxidation of cardiolipin in PRP and washed platelets.

<p>Values are presented as mean ± SEM (n = 5), expressed as percentage increase in DCF fluorescence (for ROS), percentage increase in Fura-2/AM fluorescence (intracellular calcium), Rhodamine 123 fluorescence (ΔΨ<i>m</i>) and NAO fluorescence (cardiolipin), relative to control. ***<i>p</i><0.001; significant compared to control. ^#<i>p</i><0.05, ^##<i>p</i><0.01, ^###<i>p</i><0.001; significant compared to A23187.</p

Physical and chemical characteristics of new Ibuprofen derivatives.

<p>Physical and chemical characteristics of new Ibuprofen derivatives.</p

Reaction sequence for the synthesis of new Ibuprofen derivatives.

<p>Reaction sequence for the synthesis of new Ibuprofen derivatives.</p