HPLC separation of cobalamin analogues (CorA).

<p>Corrinoids were enzymatically released from immunoprecipitated HC derived from a pool of serum from mothers (A) and cord serum (B), exposed to KCN and separated by reversed-phase HPLC at a gradient of 5–30% MeCN in 0.05 mol/L phosphoric acid pH 5 from 4 to 24 min. Corrinoids in the post column fractions were quantified with the corrinoid-assay (……) and the Cbl-assay (----). The elution pattern for CorA was calculated as the difference between the two (<b>——</b>). Three distinct CorA peaks were identified in serum from both mother and newborn eluting at 13.5, 14.5 and 16.5 min. Retention times for CNCbl (15 min) (1) and CN₂Cbi (16.5 min) (2) are indicated.</p

HC-CorA in cord blood in relation to gestational age of the newborns.

<p>The concentration of CorA in cord serum correlated to the gestational age (r² = 0.19, p = 0.0004) of the newborns (n = 63).</p

Measurements of cobalamin (HC-Cbl) and cobalamin analogues (HC-CorA) attached to serum HC.

<p>Measurements of cobalamin (HC-Cbl) and cobalamin analogues (HC-CorA) attached to serum HC.</p

Main characteristics of the mothers and the newborn babies.

<p>Main characteristics of the mothers and the newborn babies.</p

Cobalamin analogues (CorA) and cobalamins (Cbl) on haptocorrin (HC) in maternal and cord sera from 69 mother-baby pairs.

<p>(A) Results for mother and newborn showed a weak correlation for HC-CorA (p = 0.01), and (B) a strong correlation for HC-Cbl (p<0.0001). (C) The absolute and relative (HC-CorA/HC-corrinoids)) amounts (mean and SEM) of HC-CorA was higher in serum from the newborns (390±20 pmol/L, (0.62±0.016)) than in serum from the mothers (170±6 pmol/L, (0.53±0.014)), p<0.0001 (paired or unpaired t-test) for both parameters.</p