Descriptive statistics for measures of sphericity.

<p>Mean values, SEM, intra- and inter-individual standard deviation (SD) and r ratio in IgG2b isotype control samples (representative for all IgG isotype controls) of CONT.</p

Correlations between respective indicators of sphericity.

<p>Scatterplots illustrating the relation between the respective indicators of sphericity in all samples of second study part (A,B) and isotype control samples (C,D). Relation between kurtosis and SphI appears inverse proportional.</p

Longitudinal presentation of erythrocyte sphericity.

<p>Kurtosis of FSC signal of IgG2b isotype control samples of CONT subjects at all time points. Values of the respective subjects are connected by dashed lines. Solid bold line connects mean values of distribution of kurtosis at the respective time point.</p

Erythrocyte sphericity and buffer osmolarity.

<p>Kurtosis of FSC signal of erythrocytes of 10 subjects of first study part incubated in buffer mediums with varying osmolarity. With increasing osmolarity of buffer medium, sphericity of erythrocytes decreases as indicated by decreasing kurtosis, *indicates significant difference (p<0.05) to kurtosis/sphericity values at 259 mosmol/kg and ^†indicates significant difference (p<0.05) to kurtosis/sphericity values at 285 mosmol/kg (Friedman-test with adjusted pairwise comparisons).</p

Selected FSC histograms illustrating bimodal and monomodal distributions.

<p>Selected FSC histograms illustrating bimodal and monomodal distributions. x-axis displays the channels used for analysis, y-axis gives the number of events for each channel. Dashed lines indicate channel 101 chosen for calculation of SphI. The median value for the respective part of the distribution is marked by short lines. For subject 11 (Full, IgG2b, d7), kurtosis = −1.21, PCD = −0.15, SphI = 2.47 (median 1 = 148, median 2 = 60). For subject 16 (Full, IgG2b, d3), kurtosis = −0.36, PCD = −0.27, SphI = 1.70 (median 1 = 129, median 2 = 76).</p

Study design.

<p>Samples were taken at different time points after blood reinfusion. For the control group, no samples were collected at 3 (3 h) and 6 hours (6 h).</p

Results of correlation analysis between measures of sphericity.

<p>Spearman ρ correlation coefficients for the respective sphericity indicators. Values in brackets describe correlation for native erythrocytes (isotype controls).</p>*<p>Indicates significant correlation (p<0.05).</p

miRNAs among 10 tested organs.

<p>miRNAs among 10 tested organs.</p

Hematologic variables before and during 10 days after transfusion.

<p>Abbreviations : percentage of reticulocytes (Reti), lactate deshydrogenase (LDH), leukocytes (Leuk), C-reactive protein (CRP), erythrocytes (Ery), hemoglobin (Hb), erythropoietin (EPO) * and ** = significant difference (p≤0.05 and p≤0.01, resp.) from D-1 (one day before blood re-infusion).</p

ANOVA analyses of individual qPCR.

<p>ANOVA analyses of individual qPCR.</p