Axonal Regeneration and Neuronal Function Are Preserved in Motor Neurons Lacking ß-Actin In Vivo

The proper localization of ß-actin mRNA and protein is essential for growth cone guidance and axon elongation in cultured neurons. In addition, decreased levels of ß-actin mRNA and protein have been identified in the growth cones of motor neurons cultured from a mouse model of Spinal Muscular Atrophy (SMA), suggesting that ß-actin loss-of-function at growth cones or pre-synaptic nerve terminals could contribute to the pathogenesis of this disease. However, the role of ß-actin in motor neurons in vivo and its potential relevance to disease has yet to be examined. We therefore generated motor neuron specific ß-actin knock-out mice (Actb-MNsKO) to investigate the function of ß-actin in motor neurons in vivo. Surprisingly, ß-actin was not required for motor neuron viability or neuromuscular junction maintenance. Skeletal muscle from Actb-MNsKO mice showed no histological indication of denervation and did not significantly differ from controls in several measurements of physiologic function. Finally, motor axon regeneration was unimpaired in Actb-MNsKO mice, suggesting that ß-actin is not required for motor neuron function or regeneration in vivo

Pédiatrie ambulatoire à l'hôpital et à domicile.

In paediatrics, ambulatory care under hospital control is ensured both by the out-patient department and by the emergency unit. If socio-economic level and parental availability are taken into account, the hospital remains the most accessible health-care structure in large towns. The common illnesses, in most cases infectious and respiratory, should not encumber the emergency unit, but should instead be taken in charge by a structure where an extended-timetable dispensary and an out-patient clinic by appointment work in parallel. In the most favourable cases, a home care unit completes this out-patient structure. Its main characteristics are as follows: it is exclusively paediatric, and it aims at shortening or avoiding hospitalization. It improves the quality of treatment, provides the family with medico-social support and follow-up that is particularly useful in less favoured settings, and can also offer preliminary training in heavy or chronic cases. Notwithstanding its obvious advantages, budgetary cuts have led to the suppression of home care at Hôpital Saint-Pierre in 1983.SCOPUS: ar.jinfo:eu-repo/semantics/publishe

Les premiers stades de la régénération neuromusculaire après écrasement de nerf sciatique chez le rat. Etude électrophysiologique et histologique

SCOPUS: ar.jinfo:eu-repo/semantics/publishe

EVOLUTION DE LA PHOSPHATASE ALCALINE SERIQUE SOUS TRAITMENT D'ISONIAZIDE ET DE RIFAMPICINE

SCOPUS: ar.jinfo:eu-repo/semantics/publishe

Rachitismes. Classification et traitements

SCOPUS: ar.jinfo:eu-repo/semantics/publishe

Troubles de conduction dans le syndrome de kearns-sayre. Deux observations cliniques

SCOPUS: ar.jinfo:eu-repo/semantics/publishe

The early stages of neuromuscular regeneration after crushing the sciatic nerve in the rat. Electrophysiological and histological study

SCOPUS: ar.jinfo:eu-repo/semantics/publishe