A novel trityl/acridine derivatization agent for analysis of thiols by (matrix-assisted)(nanowire-assisted)laser desorption/ionization and electrospray ionization mass spectrometry

The derivatization reagent was prepared in situ by the reaction of tris(2,6-dimethoxyphenyl)methylium hexafluorophosphate with N-(2-aminoethyl)maleimide and used for the modification of a number of low molecular weight thiols. The adducts were analyzed by (MA)(NA)LDI MS and ESI MS. All registered mass spectra ((MA)(NA)LDI, ESI) revealed intense peaks of the cations of the derivatization products. The increment of the derivatization agent (499 Da) shifts the ion peaks of analytes from noisy low mass regions, thus allowing the detection of low molecular weight thiols. LDI analysis of the natural-gas condensate sample showed the presence of saturated alkylthiols (up to C22H45SH). © 2017 The Royal Society of Chemistry

A novel trityl/acridine derivatization agent for analysis of thiols by (matrix-assisted)(nanowire-assisted)laser desorption/ionization and electrospray ionization mass spectrometry

The derivatization reagent was prepared in situ by the reaction of tris(2,6-dimethoxyphenyl)methylium hexafluorophosphate with N-(2-aminoethyl)maleimide and used for the modification of a number of low molecular weight thiols. The adducts were analyzed by (MA)(NA)LDI MS and ESI MS. All registered mass spectra ((MA)(NA)LDI, ESI) revealed intense peaks of the cations of the derivatization products. The increment of the derivatization agent (499 Da) shifts the ion peaks of analytes from noisy low mass regions, thus allowing the detection of low molecular weight thiols. LDI analysis of the natural-gas condensate sample showed the presence of saturated alkylthiols (up to C22H45SH). © 2017 The Royal Society of Chemistry

1-Pyrenylboronic Acid as a Reactive Matrix for the Analysis of Polyfunctional Compounds by MALDI Mass Spectrometry: New Possibilities

Abstract 1-Pyrenylboronic acid (1-PBA) is a type of a reactive matrix useful for the analysis of polyfunctional compounds by MALDI mass spectrometry. It exhibits conventional matrix properties (absorption and transfer of laser radiation energy) and acts as a derivatizing reagent capable of interacting with vicinal functional groups under mild conditions and forming covalent adducts. Pyrenyl group in its structure, which has high ability to absorb UV laser quanta, ensures the detection of analyte derivatives containing a 1-PBA residue as radical cations, which is untypical for MALDI. The effectiveness of its use was earlier proved for compounds containing 1,2-diol fragments. This work presents additional cases of using this reactive matrix in the analysis of practically significant compounds of this type, demonstrating that 1-PBA can also react with distant OH groups. Many examples show its applicability to the study of various α-hydroxy acids and vicinal aminols and capability of forming cyclic covalent adducts with 1,2-diamines

Tris(2,6-dimethoxyphenyl)methyl carbenium ion as a charge derivatization agent for the analysis of primary amines by MALDI mass spectrometry

A novel derivatization method for the analysis of primary amines by MALDI mass spectrometry is proposed. Tris(2,6-dimethoxyphenyl) methyl carbenium cation reacts smoothly with primary amines, forming permanently charged adducts with the mass increment +359 Da and absorbance in the UV region. The approach was tested on a number of amines, including biologically active compounds and therapeutic agents. © 2016, Pleiades Publishing, Ltd

Tris(2,6-dimethoxyphenyl)methyl carbenium ion as a charge derivatization agent for the analysis of primary amines by MALDI mass spectrometry

A novel derivatization method for the analysis of primary amines by MALDI mass spectrometry is proposed. Tris(2,6-dimethoxyphenyl) methyl carbenium cation reacts smoothly with primary amines, forming permanently charged adducts with the mass increment +359 Da and absorbance in the UV region. The approach was tested on a number of amines, including biologically active compounds and therapeutic agents. © 2016, Pleiades Publishing, Ltd

Investigation of the complex antibiotic INA-5812

A concentrate with the antimicrobial activity has been isolated from the culture broth of Streptomyces roseoflavus INA-Ac-5812. Its further fractionation by reversed-phase HPLC has resulted in six fractions. It has been established by MALDI-TOF and ESI-MSn precision mass-spectrometry methods that the main components of the complex antibiotic are several closely related compounds, presumably of a glycopeptide nature. The fraction containing an individual component with a mass of 1845.788 Da has been characterized by UV/Vis absorbance and fluorescence spectra, amino acid analysis, and derivatization with tris(2,6-dimethoxyphenyl)methyl cation. The activity of fractions against pathogenic microbes has been studied. The results allow the supposition that the INA-5812 antibiotic complex is a glyco- or lipoglycopeptide antibiotic of a new type, which is very promising for further study. © 2016, Pleiades Publishing, Ltd

Investigation of the complex antibiotic INA-5812

A concentrate with the antimicrobial activity has been isolated from the culture broth of Streptomyces roseoflavus INA-Ac-5812. Its further fractionation by reversed-phase HPLC has resulted in six fractions. It has been established by MALDI-TOF and ESI-MSn precision mass-spectrometry methods that the main components of the complex antibiotic are several closely related compounds, presumably of a glycopeptide nature. The fraction containing an individual component with a mass of 1845.788 Da has been characterized by UV/Vis absorbance and fluorescence spectra, amino acid analysis, and derivatization with tris(2,6-dimethoxyphenyl)methyl cation. The activity of fractions against pathogenic microbes has been studied. The results allow the supposition that the INA-5812 antibiotic complex is a glyco- or lipoglycopeptide antibiotic of a new type, which is very promising for further study. © 2016, Pleiades Publishing, Ltd