An open-source, automated home-cage sipper device for monitoring liquid ingestive behavior in rodents

Measuring ingestive behavior of liquids in rodents is commonly used in studies of reward, metabolism, and circadian biology. Common approaches for measuring liquid intake in real time include computer-tethered lickometers or video-based systems. Additionally, liquids can be measured or weighed to determine the amount consumed without real-time sensing. Here, we built a photobeam-based sipper device that has the following advantages over traditional methods: (1) it is battery powered and fits in vivarium caging to allow home-cage measurements; (2) it quantifies the intake of two different liquids simultaneously for preference studies; (3) it is low cost and easily constructed, enabling high-throughput experiments; and (4) it is open source so that others can modify it to fit their experimental needs. We validated the performance of this device in three experiments. First, we calibrated our device using time-lapse video-based measurements of liquid intake and correlated sipper interactions with liquid intake. Second, we used the sipper device to measure preference for water versus chocolate milk, demonstrating its utility for two-bottle choice tasks. Third, we integrated the device with fiber photometry, establishing its utility for measuring neural activity in studies of ingestive behavior. This device requires no special equipment or caging, and is small, battery powered, and wireless, allowing it to be placed directly in rodent home cages. The total cost of fabrication is less than $100, and all design files and code are open source. Together, these factors greatly increase scalability and utility for a variety of behavioral neuroscience applications

An open-source, automated home-cage sipper device for monitoring liquid ingestive behavior in rodents

Measuring ingestive behavior of liquids in rodents is commonly used in studies of reward, metabolism, and circadian biology. Common approaches for measuring liquid intake in real time include computer-tethered lickometers or video-based systems. Additionally, liquids can be measured or weighed to determine the amount consumed without real-time sensing. Here, we built a photobeam-based sipper device that has the following advantages over traditional methods: (1) it is battery powered and fits in vivarium caging to allow home-cage measurements; (2) it quantifies the intake of two different liquids simultaneously for preference studies; (3) it is low cost and easily constructed, enabling high-throughput experiments; and (4) it is open source so that others can modify it to fit their experimental needs. We validated the performance of this device in three experiments. First, we calibrated our device using time-lapse video-based measurements of liquid intake and correlated sipper interactions with liquid intake. Second, we used the sipper device to measure preference for water versus chocolate milk, demonstrating its utility for two-bottle choice tasks. Third, we integrated the device with fiber photometry, establishing its utility for measuring neural activity in studies of ingestive behavior. This device requires no special equipment or caging, and is small, battery powered, and wireless, allowing it to be placed directly in rodent home cages. The total cost of fabrication is less than $100, and all design files and code are open source. Together, these factors greatly increase scalability and utility for a variety of behavioral neuroscience applications

Cultural characteristics, morphology, and variation within Claviceps africana and C. sorghi from India

Sorghum ergot in India is caused by Claviceps africana and C. sorghi. The distributions of these two species in India is not known. Eighty-nine sorghum ergot isolates were cultured from young sphacelia obtained from male sterile sorghum plants artificially inoculated using inoculum collected in the field. Based on cultural characteristics, the isolates were separated into two groups which differed distinctly in the morphology of their sphacelia, conidia, and sclerotia. Marked differences also were observed in rates of secondary conidial production and disease spread between the groups. In combination with molecular evidence, our results confirm that the isolates placed in Group I represent C. africana and Group II isolates represent C. sorghi. C. africana was found to be widely distributed in all sorghum growing areas of India. The species first described as occurring in India, C. sorghi, appears to be restricted to a few locations in the states of Maharashtra, Andhra Pradesh, and Karnataka

Prevalence of ergot of sorghum in India

This paper reports the incidence and severity of ergot (Claviceps sorghi and Claviceps africana) on sorghum (Sorghum bicolor) grown in Andhra Pradesh, Gujarat, Tamil Nadu, Maharashtra, Karnataka, Rajasthan and Uttar Pradesh from 1999 to 2002. Crops were surveyed at vegetative to physiological maturity stages and disease incidence (number of plants infected) and severity (percentage, based on the number of florets infected within panicles) recorded in 12 m2 areas. Percentage incidence of ergot infection varied with location, with Rajasthan and Gujarat recording only trace infections from 1999-2002 and Karnataka having 27-60% infection. Disease severity followed the same pattern

Prevalence of major foliar and panicle diseases of Sorghum (Sorghum bicolor [L.] Moench) in the Deccan plateau of India

Extensive on-farm disease surveys were conducted from August 1999 until March 2001 in four sorghum-growing states of the Indian Deccan plateau. A total of 965 fields were surveyed covering 228 fields in Andhra Pradesh (AP), 406 in Karnataka (KAR), 290 in Maharashtra (MH) and 41 in Tamil Nadu (TN). Among 14 foliar diseases observed, maize stripe virus (MStV), a tenuivirus transmitted by the delphacid plant hopper (Peregrinus maidis), and among five panicle diseases, ergot or sugary disease (Claviceps sorghi and C. africana) were the most destructive diseases. MStV was prevalent in 28.4% and ergot in 13.4% of the fields surveyed in two years across four states. Yet, the mean incidence of MStV in AP was 6% with 85% mean severity. The values in KAR were 12% incidence and 83% severity, in MH 5% and 67%, and in TN 12% and 76%, respectively. The mean incidence of ergot in AP was 34% with 67% mean severity. The values in KAR were 41% and 79%, in MH, 30% and 67%, and in TN 100% and 100%, respectively. Variation in frequency of occurrence of MStV was observed between 1999 and 2001. Variations in frequency could be due to weather factors, vector survival, cropping pattern, and host specificity. The frequency of ergot also was varying among years, locations, seasons and cultivars. An ergot epidemic was observed during the 1999 rainy season in Maachinenipalli village (16°35′N; 78°3′E), Andhra Pradesh. In September 2000, the disease had spread to 13 neighboring administrative zones damaging about 130 000 ha. This paper elucidates the distribution of diseases observed between 1999 and 2001 but does not imply that the diseases are restricted necessarily to a particular zone or location

MaDDOSY (Mass Determination Diffusion Ordered Spectroscopy) using an 80 MHz bench top NMR for the rapid determination of polymer and macromolecular molecular weight

YesMeasurement of molecular weight is an integral part of macromolecular and polymer characterization which usually has limitations. Herein, we present the use of a bench-top 80 MHz NMR spectrometer for diffusion-ordered spectroscopy as a practical and rapid approach for the determination of molecular weight/size using a novel solvent and polymer-independent universal calibration.Royal Society. Grant Number: URF∖R1∖180274. Engineering and Physical Sciences Research Council. Grant Numbers: EP/V037943/1, EP/V007688/1, EP/V036211/

Therapeutic hypothermia translates from ancient history in to practice

Acute postasphyxial encephalopathy around the time of birth remains a major cause of death and disability. The possibility that hypothermia may be able to prevent or lessen asphyxial brain injury is a “dream revisited”. In this review, a historical perspective is provided from the first reported use of therapeutic hypothermia for brain injuries in antiquity, to the present day. The first uncontrolled trials of cooling for resuscitation were reported more than 50 y ago. The seminal insight that led to the modern revival of studies of neuroprotection was that after profound asphyxia, many brain cells show initial recovery from the insult during a short “latent” phase, typically lasting ~6 h, only to die hours to days later during a “secondary” deterioration phase characterized by seizures, cytotoxic edema, and progressive failure of cerebral oxidative metabolism. Studies designed around this conceptual framework showed that mild hypothermia initiated as early as possible before the onset of secondary deterioration, and continued for a sufficient duration to allow the secondary deterioration to resolve, is associated with potent, long-lasting neuroprotection. There is now compelling evidence from randomized controlled trials that mild induced hypothermia significantly improves intact survival and neurodevelopmental outcomes to midchildhood

Beeldcultuur, een drieluik.I: Deconstructie van het fenomeen culturele studies

An important process in glass manufacture is the forming of the product. The forming process takes place at high rate, involves extreme temperatures and is characterised by large deformations. The process can be modelled as a coupled thermodynamical/mechanical problem including the interaction between glass, air and equipment. In this paper a general mathematical model for glass forming is derived, which is specified for different forming processes, in particular pressing and blowing. The model should be able to correctly represent the flow of the glass and the energy exchange during the process. Various modelling aspects are discussed for each process, while several key issues, such as the motion of the plunger and the evolution of the glass-air interfaces, are examined thoroughly. Finally, some examples of process simulations for existing simulation tools are provided

The contribution of X-linked coding variation to severe developmental disorders

Over 130 X-linked genes have been robustly associated with developmental disorders, and X-linked causes have been hypothesised to underlie the higher developmental disorder rates in males. Here, we evaluate the burden of X-linked coding variation in 11,044 developmental disorder patients, and find a similar rate of X-linked causes in males and females (6.0% and 6.9%, respectively), indicating that such variants do not account for the 1.4-fold male bias. We develop an improved strategy to detect X-linked developmental disorders and identify 23 significant genes, all of which were previously known, consistent with our inference that the vast majority of the X-linked burden is in known developmental disorder-associated genes. Importantly, we estimate that, in male probands, only 13% of inherited rare missense variants in known developmental disorder-associated genes are likely to be pathogenic. Our results demonstrate that statistical analysis of large datasets can refine our understanding of modes of inheritance for individual X-linked disorders