Comparative study of chemical variability of essential oils from the leaves of Lippia multiflora Mold (Verbenaceae) collected in five regions of Côte d'Ivoire

Chemical variability of a plant from various continents or collected in different localities justifies its different biological activities. Thus, the present study was undertaken to evaluate the statistical study of chemical variability of essential oils from leaves of Lippia multiflora Mold from five localities in the center of Côte d'Ivoire. Essential oils have been extracted by hydrodistillation with yields varying from 0.34 to 1.20% depending on localities. Analysis of their chemical compositions showed the predominance of eucalyptol / germacrene D (13.91 / 11.02%), α-citral / β-citral / α-phellandrene (21.87 / 16.74 / 12.79%), linalool / (±)-trans-nerolidol / α-citral (19.99 / 18.68 / 12.10%), α-citral / β-citral (17.20 / 11.59%) and α-citral / β-citral (16.38 / 11.57%) in the oils from Yamoussoukro, Tiébissou, Bouaflé, Toumodi and Dimbokro, respectively. Principal Component Analysis and Hierarchical Ascending Classification revealed that these essential oils can be classified in three groups or chemotypes dominated by Eucalyptol, Citral / Linalool and trans-Geraniol

Separation and characterization of phenolic compounds from Terminalia ivoiriensis using liquid chromatography-positive electrospray ionization tandem mass spectroscopy

Terminalia ivoriensis A. Chev. (Combretaceae) is an Ivorian medicinal plant. There is little ethnobotanical and almost no chemical information available for this species. The aim of this study was to isolate phenolic compounds from T. ivoriensis. In this way, its ethyl acetate extract (Ea) was fractionated by silica gel column chromatography followed by Sephadex LH20 filtration. Elution solvents were methanol (MeOH), methylene chloride (Mc) and ethyl acetate (EtOAc). Analysis of the obtained fractions (F1-F4) by liquid chromatography coupled to positive electro-spray ionization tandem mass spectrometry (LC-ESI-MS/MS) afforded six known polyphenols: 1) 3,3'-di-O-methylellagic acid); 2) 3,7,8-tri-O-methylellagic acid; 3) Progallin A; 4) 3,3',4-O-Trimethyl-4'-O-β-Dglucopyranosylellagic acid; 5) Punicalagin and, 6) Punicalin. All these natural products were isolated here for the first time from this plant. They presented various biological activities among which were anti-inflammatory, antioxidant and anti-HIV activities.Keywords: Terminalia, ivoriensis, LC-ESI-MS, isolation, characterization, polyphenols, activity, antiinflammatory, antioxidant, anti-HIV.African Journal of Biotechnology Vol. 12(27), pp. 4393-439

Phytochemical investigation and biological activities of Lantana rhodesiensis

peer reviewedLantana rhodesiensis Moldenke is a plant widely used to treat diseases, such as rheumatism, diabetes, and malaria in traditional medicine. To better understand the traditional uses of this plant, a phytochemical study was undertaken, revealing a higher proportion of polyphenols, including flavonoids in L. rhodesiensis leaf extract and moderate proportion in stem and root extracts. The antioxidant activity of the extracts was also determined using three different assays: the radical 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity, the FRAP method (Ferric-reducing antioxidant power) and the β-carotene bleaching test. The anti-malarial activity of each extract was also evaluated using asexual erythrocyte stages of Plasmodium falciparum, chloroquine-sensitive strain 3D7. The results showed that the leaf extract exhibited higher antioxidant and anti-malarial activities in comparison with the stem and root extracts, probably due to the presence of higher quantities of polyphenols including flavonoids in the leaves. A positive linear correlation was established between the phenolic compound content (total polyphenols including flavonoids and tannins; and total flavonoids) and the antioxidant activity of all extracts. Furthermore, four flavones were isolated from leaf dichloromethane and ethyl acetate fractions: a new flavone named rhodescine (5,6,3’,5’-tetrahydroxy-7,4’-dimethoxyflavone) (1), 5-hydroxy-6,7,3’,4’,5’-pentamethoxyflavone (2), 5-hydroxy-6,7,3′,4′-tetramethoxyflavone (3), and 5,6,3’-trihydroxy-7,4’-dimethoxyflavone (4). Their structures were elucidated by 1H, 13C NMR, COSY, HSQC, HMBC, and MS-EI spectral methods. Aside from compound 2, all other molecules were described for the first time in this plant species.EOHUB project 600873EPP-1-2018-1ES-EPPKA2-K

A New Naphthalene Derivative with Unusual Furanose Sugar from the Leaves ofDiospyros soubreana (Ebenaceae)

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ISOCOUMARINS DERIVATIVES FROM DIOSPYROS SOUBREANA (EBENACEAE

International audiencePhytochemical investigation of the leaves and the bark of trunks of Diospyros soubreana led to the isolation of three isocoumarins namely 4-O-galloylnorbergenin (1), norbergenin (2) and bergenin (3). Their structures were elucidated by spectral analysis including MS, 1D and 2D-NMR spectroscopy. All these compounds were isolated from this plant for the first time

Chemical Investigation on Leaf, Flower and Fruit Oils ofLantana camara from Côte d’Ivoire

International audienceThe compositions of essential oil obtained from leaves, flowers and fruits of Lantana camara L. growing in wild at Côte d’Ivoire, were investigated by GC and13C NMR. Thirty-seven compounds accounting for 88.3–94.5% of the oils have been identified. The leaf, flower and fruits of L. camara producedsesquiterpene-rich essential oils predominated by (E)-β-caryophyllene and α-humulene, in addition to the important content of sabinene, -phellandrene andthymol as monoterpenes. It was found the new chemical profiles of L. camar

New trans–b–bergamotene derivatives in the root andthe flower essential oils of Cyanthillium cinereum (L.) H.Rob. from C^ote d’Ivoire

International audienceRoot and flower essential oils of Cyanthillium cinereum (L.) H. Rob.(Synonym Vernonia cinerea (L.) Less.) (Asteraceae) collected inSouthern C^ote d’Ivoire was investigated using a combination ofchromatographic and spectroscopic techniques. The root oil compositionwas dominated by trans–b–bergamotene (20.7%),b–elemene (19.0%), cyperene (10.6%), germacrene A (7.1%)and b–pinene (3.8%), whereas c–humulene (31.0%), (E)–b–caryophyllene (17.0%), trans–b–bergamotene (7.7%), b–pinene(7.5%) and (E)–b–farnesene (6.0%) were the major componentsof flower oil. Two new compounds bearing the trans–b–bergamotene framework were identified: trans–b–bergamotenoneand (E)–trans–b–bergamotenol

Characterization of a new epoxy-hydroxycarvotanacetone derivative from the leaf essential oil of Laggera pterodonta from Côte d’Ivoire

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Composition and Chemical Variability of Ivorian Xylopia rubescensTrunk Bark Oil

International audienceNine samples of Xylopia rubescens Oliv. (Annonaceae) trunk bark oil have been analyzed by combination of GC(RI) and 13C NMR. The contents of the majorcomponents varied substantially from sample to sample: (8Z,11Z,14Z)-heptadeca-8,11-14-trien-2-one (14.7-33.0%), furanoguaia-1,4-diene (0-36.8%) and-pinene (0.9-29.0%). The content of a few components reached punctually an appreciable amount: -pinene (up to 10.7%), germacrene D (up to 12.0%),bicyclogermacrene (up to 10.0%) and spathulenol (up to 10.0%). X. rubescens trunk bark oil displayed a fair chemical variabilit

Composition and Chemical Variability of Enantia polycarpa Engl . & Diels Leaf Essential Oil from Côte d'Ivoire

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