Additional file 4: of Toxicity responses of Cu and Cd: the involvement of miRNAs and the transcription factor SPL7

Forward and reverse primers used to determine gene expression levels via quantitative real-time PCR. E-E-jn, exon-exon junction; E-I-b, exon-intron boundary; YLS, yellow-leaf-specific; UBQ10, ubiquitin; ACT, actin ; EF, elongation factor; APS, ATP sulfurylase; SULTR, sulfate transporter; LAC, laccase; CSD, Cu/Zn superoxide dismutase; FSD, Fe superoxide dismutase. *Pri-miRNA primer concentrations were increased to 900 nM. (DOCX 18 kb

Additional file 5: of Toxicity responses of Cu and Cd: the involvement of miRNAs and the transcription factor SPL7

Quantitative real-time PCR parameters according to the Minimum Information for publication of Quantitative real-time PCR Experiments (MIQE) guidelines derived from Bustin et al. [51]. (DOCX 16 kb

Additional file 1: of Toxicity responses of Cu and Cd: the involvement of miRNAs and the transcription factor SPL7

Cq values of 180 pri-miRNAs in leaves and roots measured using an RT-qPCR platform. (XLSX 124 kb