26 research outputs found

    Overlaid electropherograms from the analysis of unfragmented biotinylated cRNA products from the IVT reactions of the 3 different labeling kits by the Agilent 2100 Bioanalyzer

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    <p><b>Copyright information:</b></p><p>Taken from "Comparison of target labeling methods for use with Affymetrix GeneChips"</p><p>http://www.biomedcentral.com/1472-6750/7/24</p><p>BMC Biotechnology 2007;7():24-24.</p><p>Published online 18 May 2007</p><p>PMCID:PMC1885795.</p><p></p> The replicate reactions from donor A are shown for each kit: One-Cycle data represented as blue and green line; BioArray as black and orange and Superscript by the pink and turquoise lines. 1 μl of the final volume (One-Cycle = 21 μl; BioArray = 60 μl; Superscript = 100 μl) of purified IVT reaction is loaded. The RNA ladder (peaks represented in red) contains a mixture of RNAs of known concentration and size (50 (lower marker) 200, 500, 1,000, 2,000, 4,000, and 6,000 bases from left to right)

    Overlaid Bioanalyzer electropherograms for fragmented labeled cRNA targets showing the size distribution of fragmented target

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    <p><b>Copyright information:</b></p><p>Taken from "Comparison of target labeling methods for use with Affymetrix GeneChips"</p><p>http://www.biomedcentral.com/1472-6750/7/24</p><p>BMC Biotechnology 2007;7():24-24.</p><p>Published online 18 May 2007</p><p>PMCID:PMC1885795.</p><p></p> One-Cycle replicates (blue and green); Superscript replicates (orange and black); BioArray replicates (pink and turquoise). RNA ladder in red showing the lower alignment marker and the 200 and 500 base markers

    Box plots of logged probe level signal intensities for each of the GeneChip arrays included in this study

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    <p><b>Copyright information:</b></p><p>Taken from "Comparison of target labeling methods for use with Affymetrix GeneChips"</p><p>http://www.biomedcentral.com/1472-6750/7/24</p><p>BMC Biotechnology 2007;7():24-24.</p><p>Published online 18 May 2007</p><p>PMCID:PMC1885795.</p><p></p

    MA plots comparing the magnitude of change (log(signal array1) - log(signal array2)) on the y axis against the average log signal intensity (x axis)

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    <p><b>Copyright information:</b></p><p>Taken from "Comparison of target labeling methods for use with Affymetrix GeneChips"</p><p>http://www.biomedcentral.com/1472-6750/7/24</p><p>BMC Biotechnology 2007;7():24-24.</p><p>Published online 18 May 2007</p><p>PMCID:PMC1885795.</p><p></p> The green threshold lines show ± 2-fold changes. The color coding of the plot indicates the density of probesrepresented by that data point. The kits compared in each plot are given on the right hand side and the donor sample labeled by the kit is indicated at the top of each plot

    Evaluation of transcripts spiked into the total RNA sample (poly-A controls, QC3)

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    <p><b>Copyright information:</b></p><p>Taken from "Comparison of target labeling methods for use with Affymetrix GeneChips"</p><p>http://www.biomedcentral.com/1472-6750/7/24</p><p>BMC Biotechnology 2007;7():24-24.</p><p>Published online 18 May 2007</p><p>PMCID:PMC1885795.</p><p></p> Averaged signal intensity of each of the 3 GeneChips showing the standard deviation

    Bar chart showing averaged signal intensities for all internal control probesets (QC2) with corresponding standard deviations

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    <p><b>Copyright information:</b></p><p>Taken from "Comparison of target labeling methods for use with Affymetrix GeneChips"</p><p>http://www.biomedcentral.com/1472-6750/7/24</p><p>BMC Biotechnology 2007;7():24-24.</p><p>Published online 18 May 2007</p><p>PMCID:PMC1885795.</p><p></p

    The 21 differentially expressed genes identified by a class comparison test of control subjects (before and after exercise challenge (Table 2)) were clustered using a two-way hierarchical algorithm

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    <p><b>Copyright information:</b></p><p>Taken from "Exercise responsive genes measured in peripheral blood of women with Chronic Fatigue Syndrome and matched control subjects"</p><p>BMC Physiology 2005;5():5-5.</p><p>Published online 24 Mar 2005</p><p>PMCID:PMC1079885.</p><p>Copyright © 2005 Whistler et al; licensee BioMed Central Ltd.</p> In the matrix each row represents the hybridization results for a single gene, and each column represents a subject. Transcript levels are depicted as above (red) or below (green) the mean. The dendograms illustrates average-linkage hierarchical clustering of subjects (top) and genes (left). Refseq IDs for each gene is given on the right of the matrix. Those with similar exercise responses in both CFS and control subjects are at the top of the matrix, and the remainder of genes (highlighted in blue) show a diminished exercise response in CFS cases. Refseq IDs highlighted in yellow classify to the GO categories of protein or vesicle-mediated transport (Biological Process). Refseq IDs followed by: are classified to the GO category of binding (Molecular Function); and/or # are classified in the GO category of metabolism (Biological Process). The subjects group into 4 clusters which approximate to: 1) Control subjects before exercise (Con0); 2) CFS cases before exercise (CFS0); 3) Control subjects after exercise (Con24); and 4) CFS cases after exercise (CFS24)

    The three organizing principles of GO (represented by grey shaded squares) are molecular function, biological process, and cellular component

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    <p><b>Copyright information:</b></p><p>Taken from "Exercise responsive genes measured in peripheral blood of women with Chronic Fatigue Syndrome and matched control subjects"</p><p>BMC Physiology 2005;5():5-5.</p><p>Published online 24 Mar 2005</p><p>PMCID:PMC1079885.</p><p>Copyright © 2005 Whistler et al; licensee BioMed Central Ltd.</p> Related ontologies and/or subgroups of the ontologies are denoted by similarly colored squares in all tables. Ontologies presented in these figures were significant at a p-valu

    Genotyping of DENV-1 circulating in Tanzania in 2019.

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    Maximum likelihood phylogenetic tree reconstructed with the 341 sequences generated by this study and 69 additional sequences from GenBank to provide genotype reference and geographic-temporal context. The tree was rooted at midpoint. Genotype I was only detected from one sample in 2019, while genotype V was found widely circulated in the 2019 epidemic. The Tanzanian sequences (OM920075—OM920415) in red. Genotypes are presented with colored highlighted branches; Genotypes IV, III, V, II, and I are highlighted in pink, blue, green, gold, and purple, respectively. Contextual sequences are labeled with GenBank accession number, country of origin, and year of isolation.</p
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