24 research outputs found
Osmotic Stress Induced Cell Death in Wheat Is Alleviated by Tauroursodeoxycholic Acid and Involves Endoplasmic Reticulum StressâRelated Gene Expression
Although, tauroursodeoxycholic acid (TUDCA) has been widely studied in mammalian cells because of its role in inhibiting apoptosis, its effects on plants remain almost unknown, especially in the case of crops such as wheat. In this study, we conducted a series of experiments to explore the effects and mechanisms of action of TUDCA on wheat growth and cell death induced by osmotic stress. Our results show that TUDCA: (1) ameliorates the impact of osmotic stress on wheat height, fresh weight, and water content; (2) alleviates the decrease in chlorophyll content as well as membrane damage caused by osmotic stress; (3) decreases the accumulation of reactive oxygen species (ROS) by increasing the activity of antioxidant enzymes under osmotic stress; and (4) to some extent alleviates osmotic stressâinduced cell death probably by regulating endoplasmic reticulum (ER) stressârelated gene expression, for example expression of the basic leucine zipper genes bZIP60B and bZIP60D, the binding proteins BiP1 and BiP2, the protein disulfide isomerase PDIL8-1, and the glucose-regulated protein GRP94. We also propose a model that illustrates how TUDCA alleviates osmotic stressârelated wheat cell death, which provides an important theoretical basis for improving plant stress adaptation and elucidates the mechanisms of ER stressârelated plant osmotic stress resistance
Comparative Proteomic Analysis Provides New Insights Into Low Nitrogen-Promoted Primary Root Growth in Hexaploid Wheat
Nitrogen deficient environments can promote wheat primary root growth (PRG) that allows for nitrogen uptake in deep soil. However, the mechanisms of low nitrogen-promoted root growth remain largely unknown. Here, an integrated comparative proteome study using iTRAQ analysis on the roots of two wheat varieties and their descendants with contrasting response to low nitrogen (LN) stress was performed under control (CK) and LN conditions. In total, 84 differentially abundant proteins (DAPs) specifically involved in the process of LN-promoted PRG were identified and 11 pathways were significantly enriched. The Glutathione metabolism, endocytosis, lipid metabolism, and phenylpropanoid biosynthesis pathways may play crucial roles in the regulation of LN-promoted PRG. We also identified 59 DAPs involved in the common response to LN stress in different genetic backgrounds. The common responsive DAPs to LN stress were mainly involved in nitrogen uptake, transportation and remobilization, and LN stress tolerance. Taken together, our results provide new insights into the metabolic and molecular changes taking place in contrasting varieties under LN conditions, which provide useful information for the genetic improvement of root traits and nitrogen use efficiency in wheat
Melatonin Mitigates Water Deficit Stress in <i>Cenchrus alopecuroides</i> (L.) Thunb through Up-Regulating Gene Expression Related to the Photosynthetic Rate, Flavonoid Synthesis, and the Assimilatory Sulfate Reduction Pathway
Melatonin can improve plant adaptability to water deficit stress by regulating the biosynthesis of flavonoids and improving the reactive oxygen species-scavenging enzyme system. However, it remains unclear whether melatonin mitigates the effects and causes of water deficit stress in Cenchrus alopecuroides. We conducted a PEG-simulated water stress pot experiment to determine whether and how exogenous melatonin alleviates water deficit in C. alopecuroides. The experiment was divided into four treatments: (1) normal watering (Control), (2) 40% PEG-6000 treatment (D), (3) 100 ÎŒmol·Lâ1 melatonin treatment (MT), and (4) both melatonin and PEG-6000 treatment (DMT). The results showed that melatonin can alleviate water deficit in C. alopecuroides by effectively inhibiting plant chlorophyll degradation and MDA accumulation while increasing antioxidant enzyme activities and photosynthetic rates under water deficit stress. The transcriptome results indicated that melatonin regulates the expression of genes with the biosynthesis pathway of flavonoids (by increasing the expression of PAL, 4CL, HCT, and CHS), photosynthesis-antenna proteins (by increasing the expression of LHC), and sulfur metabolism (the expression of PAPSS and CysC is up-regulated in the assimilatory sulfate reduction pathway), while up-regulating the transcription factors (AP2/ERF-ERF-, C2H2-, WRKY-, Tify-, bHLH-, NAC-, and MYB-related). These findings revealed the possible causes by which melatonin mitigates water deficit stress in C. alopecuroides, which provided novel insights into the role of melatonin in water deficit stress
Asymmetric line edge roughness of multilayer grating reference materials
Line edge roughness (LER) in a one dimensional Si/SiO2 multilayer grating reference material with 20 nm nominal pitch size was investigated. It was shown for the first time that the LER of Si on SiO2 edges was about three times larger than that of SiO2 on Si edges. The asymmetric LERs led to asymmetric uncertainties and it must be considered when using the multilayer grating reference material. Moreover, the origin of asymmetric LER was investigated based on distinguishing contributions of interfacial roughness and interfacial diffusion. The interfacial roughness was determined to be symmetric because the surface roughness of the substrate, the Si single layer, the Si/SiO2 bilayer and the Si/SiO2 multilayer were almost the same with the value about 0.1 nm. Whereas, the interfacial diffusion layer of Si on SiO2 interface was about two times larger than that of SiO2 on Si interface through X-ray reflectivity (XRR) reverse fitting and transmission electron microscopy (TEM) image analysis. The asymmetric interfacial diffusion layer was proposed to be the main reason of the observed asymmetric LERs of the Si/SiO2 multilayer grating reference material
Optimization of Nano-Grating Pitch Evaluation Method Based on Line Edge Roughness Analysis
Pitch uncertainty and line edge roughness are among the critical quality attributes of a pitch standard and normally the analyses of these two parameters are separate. The analysis of self-traceable Cr atom lithography nano-gratings shows a positive relevance and sensitivity between LER and evaluated standard deviation of pitch. Therefore, LER can be used as an aided pre-evaluation parameter for the pitch calculation method, such as the gravity center method or the zero-crossing points method. The optimization of the nano-grating evaluation method helps to obtain the accurate pitch value with fewer measurements and provide a comprehensive characterization of pitch standards
Identification and characterization of circRNAs involved in the regulation of low nitrogen-promoted root growth in hexaploid wheat
Abstract Background CircRNAs are widespread in plants and play important roles in response to abiotic stresses. Low nitrogen (LN) promotes the growth of plant root system, allowing it to explore more nitrogen. However, whether circRNAs involved in the response to LN stress and the regulation of LN-promoted root growth in wheat remains unclear. Methods Two wheat varieties (LH9 and XN979) with contrasting root phenotypes to LN stress were used as materials to identify circRNAs under control and LN conditions by using high-throughput sequencing technology. Results Six differentially expressed circRNAs (DECs) involved in the common response to LN stress and 23 DECs involved in the regulation of LN-promoted root growth were successfully identified. GO analysis of the DEC-host genes involved in the regulation of LN-promoted root growth showed that GO terms related to biological regulation, responses to stimuli and signalling were significantly enriched. Moreover, seven DECs were predicted to have miRNA binding sites and may serve as miRNA sponges to capture miRNAs from their target genes. Conclusions LN stress altered the expression profiles of circRNAs in wheat. This is the first report of LN stress responsive circRNAs in plants. Our results provided new clues for investigating the functions of circRNAs in response to LN stress and in the regulation of LN-promoted wheat root growth
Identification of Two Novel Wheat Drought Tolerance-Related Proteins by Comparative Proteomic Analysis Combined with Virus-Induced Gene Silencing
Drought is a major adversity that limits crop yields. Further exploration of wheat drought tolerance-related genes is critical for the genetic improvement of drought tolerance in this crop. Here, comparative proteomic analysis of two wheat varieties, XN979 and LA379, with contrasting drought tolerance was conducted to screen for drought tolerance-related proteins/genes. Virus-induced gene silencing (VIGS) technology was used to verify the functions of candidate proteins. A total of 335 differentially abundant proteins (DAPs) were exclusively identified in the drought-tolerant variety XN979. Most DAPs were mainly involved in photosynthesis, carbon fixation, glyoxylate and dicarboxylate metabolism, and several other pathways. Two DAPs (W5DYH0 and W5ERN8), dubbed TaDrSR1 and TaDrSR2, respectively, were selected for further functional analysis using VIGS. The relative electrolyte leakage rate and malonaldehyde content increased significantly, while the relative water content and proline content significantly decreased in the TaDrSR1- and TaDrSR2-knock-down plants compared to that in non-knocked-down plants under drought stress conditions. TaDrSR1- and TaDrSR2-knock-down plants exhibited more severe drooping and wilting phenotypes than non-knocked-down plants under drought stress conditions, suggesting that the former were more sensitive to drought stress. These results indicate that TaDrSR1 and TaDrSR2 potentially play vital roles in conferring drought tolerance in common wheat
Sub-5 nm AFM Tip Characterizer Based on Multilayer Deposition Technology
Atomic force microscope (AFM) is commonly used for three-dimensional characterization of the surface morphology of structures at nanoscale, but the âInflation effectâ of the tip is an important factor affecting the accuracy. A tip characterizer has the advantages of in situ measurement, higher accuracy of probe inversion results, and relatively simple fabrication process. In this paper, we developed a rectangular tip characterizer based on multilayer film deposition technology with protruding critical dimension parts and grooves parts. And the tip characterization is highly consistent across the line widths and grooves, and still performs well even in the sub-5 nm line width tip characterizer. This indicates that tip characterizers produced by this method can synergistically meet the combined requirements of standard rectangular structure, very small line edge roughness, very small geometry dimension, and traceable measurements
QTLs for seedling traits under salinity stress in hexaploid wheat
<div><p>ABSTRACT: Soil salinity limits agricultural production and is a major obstacle for increasing crop yield. Common wheat is one of the most important crops with allohexaploid characteristic and a highly complex genome. QTL mapping is a useful way to identify genes for quantitative traits such as salinity tolerance in hexaploid wheat. In the present study, a hydroponic trial was carried out to identify quantitative trait loci (QTLs) associated with salinity tolerance of wheat under 150mM NaCl concentration using a recombinant inbred line population (Xiaoyan 54ĂJing 411). Values of wheat seedling traits including maximum root length (MRL), root dry weight (RDW), shoot dry weight (SDW), total dry weight (TDW) and the ratio of TDW of wheat plants between salt stress and control (TDWR) were evaluated or calculated. A total of 19QTLs for five traits were detected through composite interval mapping method by using QTL Cartographer version 2.5 under normal and salt stress conditions. These QTLs distributed on 12 chromosomes explained the percentage of phenotypic variation by individual QTL varying from 7.9% to 19.0%. Among them, 11 and six QTLs were detected under normal and salt stress conditions, respectively and two QTLs were detected for TDWR. Some salt tolerance related loci may be pleiotropic. Chromosome 1A, 3A and 7A may harbor crucial candidate genes associated with wheat salt tolerance. Our results would be helpful for the marker assisted selection to breed wheat varieties with improved salt tolerance.</p></div
Sub-5 nm AFM Tip Characterizer Based on Multilayer Deposition Technology
Atomic force microscope (AFM) is commonly used for three-dimensional characterization of the surface morphology of structures at nanoscale, but the “Inflation effect” of the tip is an important factor affecting the accuracy. A tip characterizer has the advantages of in situ measurement, higher accuracy of probe inversion results, and relatively simple fabrication process. In this paper, we developed a rectangular tip characterizer based on multilayer film deposition technology with protruding critical dimension parts and grooves parts. And the tip characterization is highly consistent across the line widths and grooves, and still performs well even in the sub-5 nm line width tip characterizer. This indicates that tip characterizers produced by this method can synergistically meet the combined requirements of standard rectangular structure, very small line edge roughness, very small geometry dimension, and traceable measurements