4 research outputs found
Contribution to diagnostics/prognostics of tuberculosis in children. I. New methods of assaying zinc and simultaneously copper and zinc in diluted sera by flame atomic-absorption spectrometry
In an attempt to provide a reliable status of metal ions in children, new methods of analysis of children's sera are proposed. New flame atomic-absorption spectrometric (FAAS) methods are simple, cost- and time-effective and, above all, labor-, reagent- and sample-saving. Two methods were suggested: method A for simultaneous determination of Cu and Zn from 5-fold diluted sera, and method B, for assaying zinc alone in 10-fold diluted samples. Both methods are based on a single-step sample pretreatment (deproteinization with 3 mol dmā3 HCl). Method A uses a single-step calibration with a mixed standard. The main advantage of method B is an additional reduction in sample consumption. Both methods were fully validated against reference methods. Accuracy, sensitivity and precision have proven them to be comparable to the reference methods in terms of analytical performance, and applicable to analyses of children's sera
Contribution to diagnostics/prognostics of tuberculosis in children. II. Indicative value of metal ions and biochemical parameters in serum
In an attempt to provide a reliable status of metal ions in children, new methods of analysis of children's sera are proposed. New flame atomic-absorption spectrometric (FAAS) methods are simple, cost- and time-effective and, above all, labor-, reagent- and sample-saving. Two methods were selected andvalidated against reference methods: method A for simultaneous determination of Cu and Zn from 5-fold diluted sera, and method B, for assaying zinc alone in 10-fold diluted samples. Both methods are based on a single-step sample pretreatment (deproteinization with 3 mol dm-3 HCl). Method A uses a single-step calibration with a mixed standard. The main advantage of method B is an additional reduction in sample consumption. Both methods were fully validated; accuracy, sensitivity and precision have proven them to becomparable to the reference methods, in terms of analytical performance,and applicable to analyses of children's sera
Simultaneous determination of iron and copper in children's sera by FAAS
Predložena je nova jednostavna metoda plamene atomsko-apsorpcijske spektrometrije (FAAS), za simultano odreÄivanje željeza i bakra u serumu djece. Ona se temelji na predobradbi uzorka u jednom koraku (deproteinizacija s 3 mol Lā3 HCl u odnosu 1:1) i kalibraciji u jednom koraku sa standardom pripravljenim u 1.5 mol Lā3 HCl.
Tijekom optimizacije metode primijenjen je multifaktorski dizajnirani eksperiment. PreporuÄena metoda osigurava ispravnost, osjetljivost i preciznost usporedljivu onima referentnih metoda. Novi je pristup jednostavan i brz; on Å”tedi i vrijeme i reagense i uzorke, pri Äemu je potonje posebno važno u djeÄjoj dijagnostici.A new and simple flame atomic-absorption spectrometric (FAAS) method is proposed for simultaneous determination of iron and copper in children's sera. It is based on single-step sample pretreatment (deproteinization with 3 mol Lā1 HCl, ratio 1:1) and single-step calibration using 1.5 mol Lā1 HCl standard. During methodās optimization a short multifactorial design experiment was used. The proposed method assures accuracy, sensitivity and precision comparable to that of the reference methods. The new approach is simple and time-, labour- and serum-saving, the latter being especially important in pediatric diagnostics
Contribution to diagnostics/prognostics of tuberculosis in children. I. New methods of assaying zinc and simultaneously copper and zinc in diluted sera by flame atomic-absorption spectrometry
In an attempt to provide a reliable status of metal ions in children, new methods of analysis of childrenās sera are proposed. New flame atomic-absorption spectrometric (FAAS) methods are simple, cost- and time-effective and, above all, labor-, reagent- and sample-saving. Two methods were suggested: method A for simultaneous determination of Cu and Zn from 5-fold diluted sera, and method B, for assaying zinc alone in 10-fold diluted samples. Both methods are based on a single-step sample pretreatment (deproteinization with 3 mol dmā3 HCl). Method A uses a single-step calibration with a mixed standard. The main advantage of method B is an additional reduction in sample consumption. Both methods were fully validated against reference methods. Accuracy, sensitivity and precision have proven them to be comparable to the reference methods in terms of analytical performance, and applicable to analyses of childrenās sera