Amino acid sequence alignment of the PtEMT1p from <i>P</i>. <i>tsukubaensis</i> NBRC1940 and nine homologous proteins.

<p>(I), (II) and (III) show low-identity regions I, II, and III, respectively. Identical residues are shown on a black background. GAC96558_P. hub: <i>Pseudozyma hubeiensis</i> SY62. XP_011389468_U. may: <i>Ustilago maydis</i> 521. CDR99457.1_S. sci: <i>Sporisorium scitamineum</i>. CBQ73522_S. rei: <i>Sporisorium reilianum</i> SRZ2. CDI53946_M. pen: <i>Melanopsichium pennsylvanicum</i> 4. CCF52717_U. hor: <i>Ustilago hordei</i>. ETS61959_P. aph: <i>Pseudozyma aphidis</i> DSM70725. GAK68006_P. ant: <i>Pseudozyma antarctica</i>. GAC75887_P. ant: <i>Pseudozyma antarctica</i> T-34. P. tsu: <i>Pseudozyma tsukubaensis</i> NBRC1940.</p

The biosynthetic pathway of MELs.

<p>Emt1p: erythritol/mannose transferase. Mac1p and Mac2p: acyl transferases. Mat1p: acetyl transferase. Mmf1p: putative transporter.</p

Comparison of amino acid sequence homology of PtEMT1p and homologous proteins.

<p>Comparison of amino acid sequence homology of PtEMT1p and homologous proteins.</p

Partial ¹H and ¹³C NMR spectra.

<p>(A) ¹H NMR spectra. (B) ¹³C NMR spectra. (a): conventional type of MEL-A synthesized by Δ<i>PaEMT1</i> harboring pUXV1_neo::PaEMT1, (b): MEL-A synthesized by Δ<i>PaEMT1</i> harboring pUXV1_neo::PtEMT1.</p

Chemical structure of MELs.

<p>(A) Conventional type of MELs. (B) Diastereomer type of MEL-B.</p

The plasmid maps of pUXV1_neo::PaEMT1 and pUXV1_neo::PtEMT1.

<p>The plasmid maps of pUXV1_neo::PaEMT1 and pUXV1_neo::PtEMT1.</p

The amino acid sequence identities of proteins in the MEL biosynthesis gene cluster compared to <i>U</i>. <i>maydis</i> and <i>P</i>. <i>antarctica</i>.

<p>The amino acid sequence identities of proteins in the MEL biosynthesis gene cluster compared to <i>U</i>. <i>maydis</i> and <i>P</i>. <i>antarctica</i>.</p

TLC analysis of MEL production by Δ<i>PaEMT1</i> harboring pUXV1_neo::PtEMT1.

<p>STD: standard MELs containing MEL-A, MEL-B and MEL-C. ΔPaEMT1::neo: Δ<i>PaEMT1</i> harboring pUXV1_neo. ΔPaEMT1::neo_PaEMT1: Δ<i>PaEMT1</i> harboring pUXV1_neo::PaEMT1. ΔPaEMT1::neo_PtEMT1: Δ<i>PaEMT1</i> harboring pUXV1_neo::PtEMT1. Each strain was cultivated in MEL production medium containing 10% (w/v) glucose for 7 days at 25°C. The spots were visualized using anthrone reagent.</p

Gene clusters of MEL biosynthesis.

<p>Emt1: erythritol/mannose transferase; Mac1 and Mac2: acyl transferases; Mat1: acetyl transferase; Mmf1: putative transporter.</p

Enlarged view of regions I, II and III.

<p>Enlarged view of regions I, II and III.</p