Additional file 1: Table S1. of RNA-seq-based evaluation of bicolor tepal pigmentation in Asiatic hybrid lilies (Lilium spp.)

Summary of assembled sequences from the tepal parts of the Asiatic hybrid lily Lollypop. Table S3. Primers used for quantitative RT-PCR (qRT-PCR) analysis. Figure S1. Phenylpropanoid, anthocyanin, and cinnamic acid derivative biosynthesis pathways in lily tepals. Enzymes whose genes are up-regulated in upper tepals (estimated by qRT-PCR) are shown in blue. 3GT, anthocyanidin 3-O-glucosyltransferase; 3RT, anthocyanidin-3-glucoside rhamnosyltransferase; 4CL, 4-coumaroyl: CoA-ligase; 7GT, anthocyanidin-3-rutinoside 7-glucosyltransferase; ANS, anthocyanidin synthase; CHI, chalcone isomerase; CHS, chalcone synthase; C3H, p-coumarate 3-hydroxylase; C4H, cinnamate 4-hydroxylase; DFR, dihydroflavonol 4-reductase; F3H, flavanone 3-hydroxylase; F3′H, flavonoid 3′-hydroxylase; FLS, flavonol synthase; GST, glutathione S-transferase; HCT, shikimate O-hydroxycinnamoyl transferase; MATE, multidrug and toxic compound extrusion transporter; PAL, phenylalanine ammonia-lyase. Figure S2. HPLC analysis of anthocyanins and CADs in upper tepals (upper) and tepal bases (basal) of lily cultivars. A: Absorbance at 525 nm (anthocyanins) of the tepal extracts in Lollypop, and cyanidin 3-O-glycoside (Cy3G) and cyanidin 3-O-rutinoside (Cy3R) standards. B: Absorbance at 340 nm (CADs) of the tepal extracts in six cultivars. Figure S3. Alignment of predicted amino acid sequences of isoforms annotated as LhPAL1, LhPAL2, and LhPAL3 (A), and LhCHSa and LhCHSb (B). Letters on black and grey backgrounds indicate identical and similar amino acids, respectively. Asterisks indicate stop codons. Figure S4. Relative expression levels of c30288_g1 (HCT), c10735_g1 (MYB3), c25442_g1 (MYB8), c25442_g2, c24227_g1 (R3-MYB), c24227_g2 (R3-MYB), c18278_g2 (R3-MYB), c36339_g1 (SPL9), and c16635-g1 (RCP1) in upper tepals and tepal bases of Lollypop during floral development (St 1–5). ACTIN was used to normalize the expression of target genes. Values and vertical bars indicate the mean ± standard error (n = 3). The same letters above the columns indicate that the values are not statistically significant (p <0.05) by Tukey’s HSD. Figure S5. Relative expression levels of LhMYB12 in Sugar Love and WD40 in Sugar Love and Montreux in upper tepals and tepal bases during floral development (St 1–5, A) and flowers of the cultivars Montreux and Sugar Love (B). ACTIN was used to normalize the expression of target genes. Values and vertical bars indicate the means ± standard error (n = 3). The same letters above the columns indicate that the values are not statistically significant (p <0.05) by Tukey’s HSD. Figure S6. Putative miR828 and pri-miR828 sequences in Lollypop. A: Putative miR828 and its target site appeared in c22900_g1 (MYB12). B: Sequence alignment of c13793_g1 and pri-miR828 in Glycine max [GmPri-miR828a (NR_126648) and GmPri-miR828b (NR_126651)], Vitis vinifera [VvPri-miR828a (NR_127861) and VvPri-miR828b (LM611741)], and Malus domestica [MdPri-miR828b (NR_120979) and MdPri-miR828a (NR_120978)]. (PDF 1261 kb

Additional file 2: Table S2. of RNA-seq-based evaluation of bicolor tepal pigmentation in Asiatic hybrid lilies (Lilium spp.)

Annotation and expression summary of differentially expressed genes. (XLSX 216 kb

Mosla dianthera Maxim. var. nana Ohwi

原著和名: ヒカゲヒメジソ科名: シソ科 = Labiatae採集地: 栃木県 栃木市 柏倉 (下野 栃木市 柏倉)採集日: 1988/9/13採集者: 萩庭丈壽整理番号: JH016488国立科学博物館整理番号: TNS-VS-96648

Bioactive Sesquiterpene Aryl Esters from the Culture Broth of <i>Armillaria</i> sp.

Two new compounds, 10-dehydroxymelleolide D (<b>1</b>) and 13-hydroxymelleolide K (<b>2</b>), along with seven known compounds, 5′-<i>O</i>-methylmelledonal (<b>3</b>), melleolide D (<b>4</b>), 13-hydroxydihydromelleolide (<b>5</b>), melleolide (<b>6</b>), armillarinin (<b>7</b>), armillaridin (<b>8</b>), and armillarikin (<b>9</b>), were isolated from the culture broth of <i>Armillaria</i> sp. Their structures were determined by spectroscopic data analysis. All the compounds inhibited plant growth of lettuce. Melleolide (<b>6</b>) and armillarikin (<b>9</b>) inhibited mycelial growth of <i>Coprinopsis cinerea</i> and/or <i>Flammulina velutipes</i>

Additional file 9: of Comparative whole genome re-sequencing analysis in upland New Rice for Africa: insights into the breeding history and respective genome compositions

Figure S2. Phylogeny of genes for fucogalactoxyloglucan biosynthesis. Predicted proteins sequences were aligned by ‘CLUSTAL Ω’ and a phylogenetic dendrogram was constructed by ‘Simple Phylogeny’ using the neighbor-joining method with the option of ‘exclude gaps’. Twelve fucosyltransferase genes in Arabidopsis (AtFUT1 to AtFUT12), four candidate genes for galactosyltransferase in rice, four candidate genes for acetyltransferase genes in rice were analyzed together with the five fucosyltransferase genes with CG14-alleles. The five fucosyltransferase genes of interest were marked with a circle. (PPTX 85 kb

Additional file 4: of Comparative whole genome re-sequencing analysis in upland New Rice for Africa: insights into the breeding history and respective genome compositions

Table S3. Distribution of InDel length. (XLSX 12 kb

Additional file 2: of Comparative whole genome re-sequencing analysis in upland New Rice for Africa: insights into the breeding history and respective genome compositions

Figure S1. k-mer analysis (k-merlength = 17). The X-axis shows k-mer depth, and the Y-axis shows proportion that represents the frequency at that k-mer depth divided by to total frequency of all k-mer depth. (a) WAB56–104, (b) CG14, (c) NERICA 3, (d) NERICA 4, (e) NERICA 5, (f) NERICA 7. Arrow indicates a peak of heterogeneous sequences.Peak between 0 to 8 of k-mer depth would be due to sequencing error or contaminants. (PPTX 109 kb

Reversible Complexation Mediated Living Radical Polymerization (RCMP) Using Organic Catalysts

A novel class of living radical polymerization using amines as organic catalysts was developed. It is based on a new reversible activation mechanism, <i>reversible complexation </i>(<i>RC</i>). The polymer molecular weight and its distribution (<i>M</i>_w/<i>M</i>_n = 1.1–1.4) were well controlled in the polymerizations of methyl methacrylate (MMA), styrene, acrylonitrile, and some functional methacrylates with a fairly high conversion in hours in many cases. The catalysts include such common amines as triethylamine and tetramethylethylenediamine (TMEDA). Their low cost, good environmental safety, and ease of handling may be attractive for possible applications. Kinetic studies supported the RC mechanism. The activation rate constant for the MMA/TMEDA system was large enough to explain why the system provides low-polydispersity polymers from an early stage of polymerization

Additional file 7: of Comparative whole genome re-sequencing analysis in upland New Rice for Africa: insights into the breeding history and respective genome compositions

Table S6. Common introgression segments from CG 14. (XLSX 104 kb

Additional file 16: of Comparative whole genome re-sequencing analysis in upland New Rice for Africa: insights into the breeding history and respective genome compositions

Table S12. Summary of genome assembly and annotation of unmapped reads from WAB 56–104. (XLSX 11 kb