Escherichia coli dominance and antimicrobial resistance in urinary tract infections among diabetic patients: Insights from Birnin Kebbi Metropolis, Nigeria

Background: The global rise in antibiotic-resistant urinary tract infections (UTIs) is a growing concern, particularly among diabetic patients. This study examines the antibiotic resistance patterns of bacterial uropathogens in diabetic patients at Sir Yahaya Memorial Hospital in Birnin Kebbi. Methods: A purposive sampling approach was used to collect 51 mid-stream urine samples in sterile containers. Cultural and biochemical methods were employed for the isolation and identification of uropathogenic bacteria. Antibiotic sensitivity testing was performed using the disc diffusion method, with results interpreted according to the Clinical Laboratory Standards Institute (CLSI) guidelines. Results: UTIs were prevalent in 23.5% (12/51) of the samples. Escherichia coli was the most prevalent uropathogen, accounting for 41.3% (7/17) of cases, followed by Klebsiella pneumoniae at 23.5% (4/17). Staphylococcus aureus and Proteus mirabilis each contributed to 17.6% (3/17) of cases. Notably, E. coli and K. pneumoniae exhibited 100% resistance to chloramphenicol and sparfloxacin, respectively. Conclusion: These findings underscore the need for further molecular research to characterize these uropathogens and identify the genes contributing to antibiotic resistance

Bacteriological and Physicochemical Study of Retailed Cow Milk collected from Different Locations in Birnin Kebbi

Abstract Milk is an excellent source of both major and minor components needed to meet the nutritional requirements of the human body. The current study was conducted to evaluate the bacteriological and physicochemical quality of cow milk collected from various locations in Birnin Kebbi.  A total of twenty-four (24) milk samples (six from each location) were purchased. All samples were subjected to bacteriological analysis using total plate count, and physicochemical composition analysis, which included PH, moisture content, fat content, protein content, and mineral determination (calcium, sodium, and potassium). The mean range for Total Plate Count is 1.83x106-3.42x106 CFU/ML. Furthermore, bacteria isolated and identified from milk samples include Streptococcus spp, Shigella spp, Escherichia coli, Bacillus spp, Staphylococcus aureus, and Proteus spp. The physicochemical analysis results indicated that the mean range is PH (4.87±0.04-5.52±0.05) Moisture content (82.22±0.60-87.50±2.16), Fat content (0.56±0.02-1.84±0.01), Protein content (11.27±4.4-12.55±4.99), Calcium (173.83±4.43-475.67±22.24), Sodium (11.83±4.26- 111±12.44) and Potassium (131±9.12-596±6.16) respectively. The study's findings highlight the poor bacteriological quality of milk, as evidenced by a higher Total Plate Count. Moreover, the presence of pathogenic microbes in milk, such as Bacillus spp, Shigella spp, and Escherichia coli, is likely to pose a serious public health risk. As a result, it is recommended that proper hygiene practices be implemented, as well as the incorporation of effective monitoring throughout the production to delivery chain. &nbsp