Additional file 2: Table S1. of Endothelial responses of the alveolar barrier in vitro in a dose-controlled exposure to diesel exhaust particulate matter

Differential gene expression observed in EA.hy 926 cells exposed for 6 and 24 h to AAPH and tBHQ at different concentrations. The response factors are ratios between the gene expression levels (calculated relatively to the housekeeping genes) after exposure related to their respective solvent control (mean ± SEM, n = 3). Bold letters indicate significant differences between the evaluated time-points (P < 0.05 with a fold change greater than 2). (PDF 15 kb

Additional file 1: Figure S1. of Endothelial responses of the alveolar barrier in vitro in a dose-controlled exposure to diesel exhaust particulate matter

Nuclear translocation of Nrf2 in endothelial EA.hy 926 cells in monoculture after treatment with 20 mM AAPH for 2 h. EA.hy 926 cells were exposed to 20 mM AAPH for 2 h. Cells kept in complete medium without AAPH served as solvent control. Nrf2 translocation was evaluated for 2 h after treatment. Cells were stained with anti-Nrf2 antibody (green), nuclei are counterstained with Hoechst 33342 (blue). Images were analyzed using a Zeiss LSM 510 META. 2 h after exposure almost every endothelial cells shows strong colocalization of Nrf2 with the nucleus (red arrows). In control EA.hy 926 cells, no nuclear translocation was observed (compare white arrows). (PDF 13756 kb

Additional file 3: Table S2. of Endothelial responses of the alveolar barrier in vitro in a dose-controlled exposure to diesel exhaust particulate matter

Secretion of second messengers after exposure of the tetraculture to different doses of DEPM at different time-points. Tetracultures were exposed to 80 ng/cm2 or 240 ng/cm2 of diesel exhaust particles. Aliquots of the undernatant were taken at 6, 24 and 48 h after exposure and stored upon analysis at -80 °C. The level of secreted second messengers was evaluated using a multiplexed assay from Mesoscale Diagnostics for pro-inflammatory cytokines (A), cytokines (B) and chemokines (C). Tetracultures that were kept in the aerosol chamber for the same exposure time but with the particle generator in stand-by mode served as control. Data represents the mean of at least four Transwell™ inserts ± SEM. (PDF 125 kb

Additional file 1: Figure S1. of Effects of silver nanoparticles and ions on a co-culture model for the gastrointestinal epithelium

Mucus layer characterization (Alcian blue staining). Figure S2. Mucus layer characterization (Toluidine blue staining and TEM). Figure S3. Mucus layer characterization (Toluidine blue staining, top view). Figure S4. Cell monolayer integrity evaluation (TEER). Figure S5. Cell-free DCFH-DA assay. Figure S6. TEM images of cells in co-culture exposed to Ag particles. Figure S7. Hierarchical clustering. Table S1. Detailed information on protein identification. Table S2. Cellular Ag content determination. Table S3. KEGG enrichment analysis. (DOCX 1.17 mb