A multi-wavelength view on the dusty Wolf-Rayet star WR 48a

We present results from the first attempts to derive various physical characteristics of the dusty Wolf-Rayet star WR 48a based on a multi-wavelength view of its observational properties. This is done on the basis of new optical and near-infrared spectral observations and on data from various archives in the optical, radio and X-rays. The optical spectrum of WR 48a is acceptably well represented by a sum of two spectra: of a WR star of the WC8 type and of a WR star of the WN8h type. The strength of the interstellar absorption features in the optical spectra of WR 48a and the near-by stars D2-3 and D2-7 (both members of the open cluster Danks 2) indicates that WR 48a is located at a distance of ~4 kpc from us. WR 48a is very likely a thermal radio source and for such a case and smooth (no clumps) wind its radio emission suggests a relatively high mass-loss rate of this dusty WR star (dM/dt = a few x 10^(-4) solar masses per year). Long timescale (years) variability of WR 48a is established in the optical, radio and X-rays. Colliding stellar winds likely play a very important role in the physics of this object. However, some LBV-like (luminous blue variable) activity could not be excluded as well.Comment: Accepted for publication in MNRAS; 16 pages, 16 figures, 6 table

Disentangling Subpopulations in Single-Molecule FRET and ALEX Experiments with Photon Distribution Analysis

AbstractAmong the advantages of the single-molecule approach when used to study biomolecular structural dynamics and interaction is its ability to distinguish between and independently observe minor subpopulations. In a single-molecule Förster resonance energy transfer (FRET) and alternating laser excitation diffusion experiment, the various populations are apparent in the resultant histograms. However, because histograms are calculated based on the per-burst mean FRET and stoichiometry ratio and not on the internal photon distribution, much of the acquired information is lost, thereby reducing the capabilities of the method. Here we suggest what to our knowledge is a novel statistical analysis tool that significantly enhances these capabilities, and we use it to identify and isolate static and dynamic subpopulations. Based on a kernel density estimator and a proper photon distribution analysis, for each individual burst, we calculate scores that reflect properties of interest. Specifically, we determine the FRET efficiency and brightness ratio distributions and use them to reveal 1), the underlying structure of a two-state DNA-hairpin and a DNA hairpin that is bound to DNA origami; 2), a minor doubly labeled dsDNA subpopulation concealed in a larger singly labeled dsDNA; and 3), functioning DNA origami motors concealed within a larger subpopulation of defective motors. Altogether, these findings demonstrate the usefulness of the proposed approach. The method was developed and tested using simulations, its rationality is described, and a computer algorithm is provided

Electrodelivery of Drugs into Cancer Cells in the Presence of Poloxamer 188

In the present study it is shown that poloxamer 188, added before or immediately after an electrical pulse used for electroporation, decreases the number of dead cells and at the same time does not reduce the number of reversible electropores through which small molecules (cisplatin, bleomycin, or propidium iodide) can pass/diffuse. It was suggested that hydrophobic sections of poloxamer 188 molecules are incorporated into the edges of pores and that their hydrophilic parts act as brushy pore structures. The formation of brushy pores may reduce the expansion of pores and delay the irreversible electropermeability. Tumors were implanted subcutaneously in both flanks of nude mice using HeLa cells, transfected with genes for red fluorescent protein and luciferase. The volume of tumors stopped to grow after electrochemotherapy and the use of poloxamer 188 reduced the edema near the electrode and around the subcutaneously growing tumors