2 research outputs found
Nucleotides Bearing Red Viscosity-Sensitive Dimethoxy-Bodipy Fluorophore for Enzymatic Incorporation and DNA Labeling
Nucleosides and 2′-deoxyribonucleoside
triphosphates
(dNTPs)
bearing 3,3′-dimethoxy-2,2′-diphenyl-6-(4-hydroxyphenyl)-bodipy
fluorophore attached through a propargyl or propargyl-triethylene
glycol linker to position 5 of 2′-deoxycytidine were designed
and synthesized. They exerted bright red fluorescence and good sensitivity
to viscosity changing their lifetime from 1.6 to 4.5 ns. The modifed
dNTPs were substrates for DNA polymerases and were used in enzymatic
synthesis of labeled DNA through primer extension. The modified DNA
probes served as viscosity sensors responding to protein binding by
changes of lifetime. The nucleotide with longer linker (dCpegMOBTP) was transported to
live cells and incorporated into the genomic DNA, which can be useful
for staining of DNA and imaging of DNA synthesis