Identification of SELDI peaks differentiating KD and FC in three studies.

<p>Identification of SELDI peaks differentiating KD and FC in three studies.</p

Correlation of MSIA and SELDI peak intensities.

<p>The averaged MSIA peak intensities for SAA₇₈₆₀ are plotted against the averaged SELDI peak intensities obtained during discovery studies. KD subjects are shown as solid black circles, and FC subjects are shown as open red circles. The linear regression line (solid line) with 95% CI (dotted lines) is shown.</p

Partial purification of the 7,860 Da peak.

<p>The peak is shown at mass = 7,860 Da on CM10 ProteinChip arrays (A) in the pH 9 fraction after application of KD and FC plasma to Q anion exchange ceramic beads at pH 9; (B) in 50% ACN reverse phase fraction after application of the KD sample from (A) to PLRP beads, (C) the KD sample after using an ultrafiltration centrifugation device, and (D) the KD sample after elution from SDS-PAGE band. The FC spectrum is shown only for (A).</p

SELDI peak intensities for SAA₇₈₆₀ peptide in the Validation cohort.

<p>The mean and 95% CI for the SELDI intensities of the SAA₇₈₆₀ in 15 KD and 9 FC subjects are shown for EDTA (FCe and KDe, left axis) and protease inhibitor (FCpi and KDpi) plasma (right axis).</p

Correlations of SAA₇₈₆₀ peptide with clinical laboratory test results in confirmation cohort.

<p>Correlations of SAA₇₈₆₀ peptide with clinical laboratory test results in confirmation cohort.</p

Correlation of Days of Illness at time of study vs SAA₇₈₆₀ peptide peak intensity.

<p>SELDI SAA₇₈₆₀ peak intensities are plotted versus days of illness at the time of study for KD and FC subjects in Cohort 2. KD, open circles; FC, closed red circles. Linear estimate lines with 95% CI are shown in black for KD and red for FC subjects.</p

SELDI peak intensities for Fx1 CM10 7,860 Da peak in Cohort 1.

<p>A) Means and 95% confidence interval (CI) for 10 KD and 10 FC subjects in the Discovery cohort are shown. B) Mean and 95% CI for 43 KD, 42 FC and 17 KDsa from the Confirmation cohort are shown. C) SELDI intensities for the 7,860 Da peak of 17 KD subjects during their acute and subacute phases of illness, from the Confirmation cohort. The acute and subacute values for 17 KD subjects are connected with a line.</p

Sequences of peptides from 7,860 Da protein.

<p>Sequences of peptides from 7,860 Da protein.</p

MSIA for SAA.

<p>Upper spectra: the SAA₇₈₆₀ peaks are shown in the expanded inset of 7,800–8,200 Da on the left, and circulating SAA1 and SAA2 are shown in the expanded inset of 11,400–11,800 Da on the right. The averaged spectra for replicate samples are red for KD subjects and black for FC subjects. Lower spectra: MSIA using anti-SAA IgG₁ in averaged replicate spectra of a KD subject are shown in red, and MSIA spectra of the same KD subject using an irrelevant mouse IgG₁ antibody are shown in black. The SAA₇₈₆₀ peak is shown in the expanded inset of 7,800–8,200 Da. The irrelevant IgG₁ does not detect either the circulating form of SAA or the SAA₇₈₆₀ peptide.</p

Demographic and laboratory characteristics of study subjects.

<p>Demographic and laboratory characteristics of study subjects.</p