Mutants of <i>L</i>. <i>plantarum</i> NCFB 965 and <i>W</i>. <i>viridescens</i> NCFB 1655 with increased resistance against plantaricin JK.

<p>Mutants of <i>L</i>. <i>plantarum</i> NCFB 965 and <i>W</i>. <i>viridescens</i> NCFB 1655 with increased resistance against plantaricin JK.</p

Side view of the I-TASSER-predicted structure of the APC family protein of <i>W</i>. <i>viridescens</i> NCFB 1655.

<p>The mutated amino acids are highlighted in red. The TM helix 10 is colored in light orange, TM helix 11 in cyan and TM helix 12 in marine blue. The structure is based on a known crystal structure from another APC superfamily member [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0185279#pone.0185279.ref031" target="_blank">31</a>].</p

Overview of mutations identified in mutants that are not present in the wild type strains of <i>L</i>. <i>plantarum</i> NCFB 965 and <i>W</i>. <i>viridescens</i> NCFB 1655.

<p>Overview of mutations identified in mutants that are not present in the wild type strains of <i>L</i>. <i>plantarum</i> NCFB 965 and <i>W</i>. <i>viridescens</i> NCFB 1655.</p

A consensus sequence illustrating the conserved residues in the regions of interest in the membrane protein.

<p>The consensus sequence using WebLogo (<a href="http://weblogo.berkeley.edu" target="_blank">weblogo.berkeley.edu</a>) was generated through a search with protein-BLAST using the APC family protein from <i>W</i>. <i>viridescens</i> NCFB 1655 as a template. The alignments reveal a highly conserved region spanning almost the entire TM helix 10 and to a lesser extent the loop between TM helix 11 and 12. The predicted TM helices 10–12 (from left to right) are indicated below as purple bars.</p

Genotyping of the coding region of by T-track sequencing on Alf Express™

<p><b>Copyright information:</b></p><p>Taken from "Single-track sequencing for genotyping of multiple SNPs in the N-acetyltransferase 1 (NAT1) gene"</p><p>BMC Biotechnology 2004;4():28-28.</p><p>Published online 25 Nov 2004</p><p>PMCID:PMC544357.</p><p>Copyright © 2004 Soucek et al; licensee BioMed Central Ltd.</p> (A) Displayed area from T382 to T417 (B) Displayed area from T531 to T582 (C) Displayed area from T618 to T661, with genotypes ; (D) ; (E) ; (F) – Displayed area from T735 to T797, and alleles not found

Alleles in 3'-untranslated region assessed by single A-track sequencing on Alf Express™

<p><b>Copyright information:</b></p><p>Taken from "Single-track sequencing for genotyping of multiple SNPs in the N-acetyltransferase 1 (NAT1) gene"</p><p>BMC Biotechnology 2004;4():28-28.</p><p>Published online 25 Nov 2004</p><p>PMCID:PMC544357.</p><p>Copyright © 2004 Soucek et al; licensee BioMed Central Ltd.</p> Displayed area from A1038 to A1104. (A) – wild type; (B) and heterozygote; (C) and homozygote; (D) allele; (E) allele