Boid inclusion body disease : the enigma unraveled?

Boid inclusion body disease is a worldwide occurring disease in boas and pythons. The identity of the etiological agent of the disease remained elusive for several decades. Recently however, contemporary diagnostic methods and experimental studies delivered compelling proof that a group of divergent reptarenaviruses arc the causative agents of BIBD. In contrast to what was initially thought, boid snakes can be persistent carriers of the viral disease without showing clinical signs. Although the cause of BIBD has been demonstrated, the diagnosis of BIBD may be challenging depending on the involved snake species, and the differentiation between comorbidities related to BIBD and clinical disease caused by concurrent disorders remains problematic. In this article, the course of BIBD infection in boid snakes, the major differences that are encountered towards the diagnosis of BIBD in pythons versus boas and the overall management of BIBD in captive snake collections are described

Osteosynthese van een mid-diafysaire femurfractuur met behulp van type I 'tie-in-fixator' bij een kookaburra (Dacelo novaeguineae)

In this case report, the successful management of a traumatic fracture of the femur in a kookaburra using a type I tie-in-fixator (TIF) is described. A domesticated, five-month-old kookaburra (Dacelo novaeguineae) suffered trauma due to interspecies aggression. Clinical and radiographic examination showed a closed, multiple transverse, spiral femoral fracture, located in the mid-diaphysis. Reduction of the fracture was managed using a type I TIF, combining an intra-medullar (IM) pin in combination with an external skeletal fixator. Postoperative radiographs confirmed an optimal reduction of the fracture and a correct placement of the type I TIF. Recovery was uneventful and correct positioning and normal functioning of the leg were observed immediately following surgery. One week postoperatively, the bird removed the external fixation but this did not interfere with the acquired stability and positioning of the affected leg. Two weeks after the surgery, radiographs confirmed a good healing of the fracture as callus development and bridging of the cortices were present. Based on the clinical and radiographic findings, it was decided to remove the IM pin. During a three-month follow-up period, the kookaburra showed full recovery

Detection of arenavirus in a peripheral odontogenic fibromyxoma in a red tail boa (Boa constrictor constrictor) with inclusion body disease

A captive bred red tail boa (Boa constrictor constrictor) was presented with a large intraoral mass originating from the buccal gingiva, attached to the right dentary teeth row. Based on the clinical features and histological examination, the diagnosis of a peripheral odontogenic fibromyxoma was made. Sections of liver biopsies and circulating lymphocytes contained relatively few eosinophilic intracytoplasmic inclusion bodies, indistinguishable from those observed in inclusion body disease-affected snakes. Inclusion bodies were not observed in cells comprising the neoplastic mass. Using reverse transcription polymerase chain reaction (RT-PCR), arenavirus was detected in the neoplastic tissue. Two years after surgical removal of the mass, recurrence of the neoplastic lesion was observed. Numerous large inclusion body disease inclusions were abundantly present in the neoplastic cells of the recurrent fibromyxoma. Sections of liver biopsies and circulating lymphocytes contained relatively few intracytoplasmic inclusions. The RT-PCR revealed the presence of arenavirus in blood, a liver biopsy, and neoplastic tissue. The present case describes the co-occurrence of an arenavirus infection and an odontogenic fibromyxoma in a red tail boa

Prevalence of inclusion body disease and associated comorbidity in captive collections of boid and pythonid snakes in Belgium

Inclusion body disease (IBD) is caused by reptarenaviruses and constitutes one of the most notorious viral diseases in snakes. Although central nervous system disease and various other clinical signs have been attributed to IBD in boid and pythonid snakes, studies that unambiguously reveal the clinical course of natural IBD and reptarenavirus infection are scarce. In the present study, the prevalence of IBD and reptarenaviruses in captive snake collections and the correlation of IBD and reptarenavirus infection with the clinical status of the sampled snakes were investigated. In three IBD positive collections, long-term follow-up during a three- to seven-year period was performed. A total of 292 snakes (178 boas and 114 pythons) from 40 collections in Belgium were sampled. In each snake, blood and buffy coat smears were evaluated for the presence of IBD inclusion bodies (IB) and whole blood was tested for reptarenavirus RNA by RT-PCR. Of all tested snakes, 16.5% (48/292) were positive for IBD of which all were boa constrictors (34.0%; 48/141) and 17.1% (50/292) were reptarenavirus RT-PCR positive. The presence of IB could not be demonstrated in any of the tested pythons, while 5.3% (6/114) were reptarenavirus positive. In contrast to pythons, the presence of IB in peripheral blood cells in boa constrictors is strongly correlated with reptarenavirus detection by RT-PCR (P<0.0001). Although boa constrictors often show persistent subclinical infection, long-term follow-up indicated that a considerable number (22.2%; 6/27) of IBD/reptarenavirus positive boas eventually develop IBD associated comorbidities

Prolonged environmental persistence requires efficient disinfection procedures to control Devriesea agamarum associated disease in lizards

Aims: Devriesea agamarum infection causes chronic proliferative dermatitis, especially in desert dwelling lizards. The present study was concerned with evaluating persistency of D. agamarum in the environment and the evaluation of the efficacy of various disinfection procedures. Methods and Results: First, the survival of D. agamarum was assessed both in dermal crusts obtained from clinically and naturally infected lizards, and during periods of prolonged nutrient starvation on dry surface, in moist sand and in distilled water. Secondly, a modified European Suspension Test was performed to determine the efficacy of eight procedures for the disinfection of equipment, environmental surfaces and the topical treatment of D. agamarum-associated dermal lesions. The bacterium proved to persist and remain viable for up to 57 days in dermal crusts and for more than 5 months in moist sand and distilled water. In contrast, survival on dry surfaces was limited. The results of the described dilution-neutralization method demonstrated that most of the tested disinfection procedures were sufficient in achieving a 5-decimal logarithmic reduction in the number of D. agamarum colony-forming units. The use of relatively low concentrations of hydrogen peroxide and a boric and peracetic acid solution on the other hand resulted in insufficient reduction in viable counts. Conclusions: Devriesea agamarum can persist for long periods of time in the environment, especially under moist conditions, making the use of suitable disinfection procedures necessary. Significance and Impact of the Study: This study demonstrates the need for a dry environment for most desert lizards and the use of effective disinfection procedures next to antimicrobial treatment to eliminate D. agamarum-associated disease from captive saurian collections

An atypical case of proventricular dilatation in a Red-and-green Macaw (Ara Chloropterus)

A Red-and-green Macaw (Ara chloropterus) was presented with regurgitation and passage of undigested seeds in the feces. Radiographic examination revealed dilatation of the proventriculus. Contrast radiography was performed and revealed that the proventricular dilatation and associated clinical signs resulted from circumferential thickening of the proventricular wall leading to severe narrowing of the lumen of the proventriculus. Testing for parrot bornavirus (PaBV) was negative. After the initiation of antimicrobial treatment because of suspected bacterial overgrowth, all clinical signs resolved two weeks after the start of the treatment, and radiographs taken four months after initial presentation revealed a normal appearance and size of the proventriculus. In the present case, bacterial proventriculitis associated with hyperplasia of the proventricular wall was put forward as a final but presumptive diagnosis highlighting the need to differentiate bacterial proventriculitis from other causes of proventricular dilatation in macaws

An atypical case of proventricular dilatation disease in a red-and-green macaw (Ara chloropterus)

A Red-and-green Macaw (Ara chloropterus) was presented with regurgitation and passage of undigested seeds in the feces. Radiographic examination revealed dilatation of the proventriculus. Contrast radiography was performed and revealed that the proventricular dilatation and associated clinical signs resulted from circumferential thickening of the proventricular wall leading to severe narrowing of the lumen of the proventriculus. Testing for parrot bornavirus (PaBV) was negative. After the initiation of antimicrobial treatment because of suspected bacterial overgrowth, all clinical signs resolved two weeks after the start of the treatment, and radiographs taken four months after initial presentation revealed a normal appearance and size of the proventriculus. In the present case, bacterial proventriculitis associated with hyperplasia of the proventricular wall was put forward as a final but presumptive diagnosis highlighting the need to differentiate bacterial proventriculitis from other causes of proventricular dilatation in macaws

Stress in wild and captive snakes : quantification, effects and the importance of management

As in other animals, distress and impaired welfare have a deleterious effect on the mental, physical and behavioral health of snakes in the wild and in captivity. Besides anthropogenic disturbance, the availability of food and shelter, the presence of predators, and environmental factors, such as seasonality and climatological changes, are important factors that affect the stress level and subsequent welfare in wild snake populations. In captive snakes, inappropriate management is the most prominent cause of chronic stress and impaired welfare. Chronic stress can be assumed by looking at the snake's behavior, but there is need for a standardized quantification method to pin-point more accurately (chronic) stress levels. The biomarker suitable in this framework is the level of corticosterone in plasma, feces and shed skin